Analysis of the cDNAs of Hypothetical Genes on Arabidopsis Chromosome 2 Reveals Numerous Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

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Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus.

Genetics ◽

10.1093/genetics/122.3.669 ◽

1989 ◽

Vol 122 (3) ◽

pp. 669-679

Author(s):

L D Siracusa ◽

A M Buchberg ◽

N G Copeland ◽

N A Jenkins

Keyword(s):

Molecular Markers ◽

Inbred Strain ◽

Mouse Chromosome ◽

Recombinant Inbred ◽

Recombinant Inbred Strain ◽

Interspecific Backcross ◽

Chromosome 2 ◽

Chromosome 4 ◽

Agouti Locus ◽

Mouse Chromosome 2

Abstract Recombinant inbred strain and interspecific backcross mice were used to create a molecular genetic linkage map of the distal portion of mouse chromosome 2. The orientation and distance of the Ada, Emv-13, Emv-15, Hck-1, Il-1a, Pck-1, Psp, Src-1 and Svp-1 loci from the beta 2-microglobulin locus and the agouti locus were established. Our mapping results have provided the identification of molecular markers both proximal and distal to the agouti locus. The recombinants obtained provide valuable resources for determining the direction of chromosome walking experiments designed to clone sequences at the agouti locus. Comparisons between the mouse and human genome maps suggest that the human homolog of the agouti locus resides on human chromosome 20q. Three loci not present on mouse chromosome 2 were also identified and were provisionally named Psp-2, Hck-2 and Hck-3. The Psp-2 locus maps to mouse chromosome 14. The Hck-2 locus maps near the centromere of mouse chromosome 4 and may identify the Lyn locus. The Hck-3 locus maps near the distal end of mouse chromosome 4 and may identify the Lck locus.

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cot1: A Regulator of Arabidopsis Trichome Initiation

Genetics ◽

10.1093/genetics/149.2.565 ◽

1998 ◽

Vol 149 (2) ◽

pp. 565-577

Author(s):

Daniel B Szymanski ◽

Daniel A Klis ◽

John C Larkin ◽

M David Marks

Keyword(s):

Cell Fate ◽

Gene Dosage ◽

Signal Transduction Pathway ◽

Spatial Arrangement ◽

Complex Signal ◽

Chromosome 2 ◽

Wild Type ◽

Trichome Formation ◽

In The Wild ◽

Leaf Trichome

Abstract In Arabidopsis, the timing and spatial arrangement of trichome initiation is tightly regulated and requires the activity of the GLABROUS1 (GL1) gene. The COTYLEDON TRICHOME 1 (COT1) gene affects trichome initiation during late stages of leaf development and is described in this article. In the wild-type background, cot1 has no observable effect on trichome initiation. GL1 overexpression in wild-type plants leads to a modest number of ectopic trichomes and to a decrease in trichome number on the adaxial leaf surface. The cot1 mutation enhances GL1-overexpression-dependent ectopic trichome formation and also induces increased leaf trichome initiation. The expressivity of the cot1 phenotype is sensitive to cot1 and 35S::GL1 gene dosage, and the most severe phenotypes are observed when cot1 and 35S::GL1 are homozygous. The COT1 locus is located on chromosome 2 15.3 cM north of er. Analysis of the interaction between cot1, try, and 35S::GL1 suggests that COT1 is part of a complex signal transduction pathway that regulates GL1-dependent adoption of the trichome cell fate.

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Epistatic Control of Non-Mendelian Inheritance in Mouse Interspecific Crosses

Genetics ◽

10.1093/genetics/143.4.1739 ◽

1996 ◽

Vol 143 (4) ◽

pp. 1739-1752 ◽

Cited By ~ 2

Author(s):

Xavier Montagutelli ◽

Rowena Turner ◽

Joseph H Nadeau

Keyword(s):

X Chromosome ◽

Genetic Basis ◽

Mendelian Inheritance ◽

The Other ◽

Interspecific Crosses ◽

Chromosome 2 ◽

Mus Spretus ◽

F1 Hybrid ◽

Strong Deviation ◽

Marker Loci

Abstract Strong deviation of allele frequencies from Mendelian inheritance favoring Mus spretus-derived alleles has been described previously for X-linked loci in four mouse interspecific crosses. We reanalyzed data for three of these crosses focusing on the location of the gene(s) controlling deviation on the X chromosome and the genetic basis for incomplete deviation. At least two loci control deviation on the X chromosome, one near Xist (the candidate gene controlling X inactivation) and the other more centromerically located. In all three crosses, strong epistasis was found between loci near Xist and marker loci on the central portion of chromosome 2. The mechanism for this deviation from Mendelian expectations is not yet known but it is probably based on lethality of embryos carrying particular combinations of alleles rather than true segregation distortion during oogenesis in F1 hybrid females.

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