scholarly journals The rolB Gene of Agrobacterium rhizogenes Does Not Increase the Auxin Sensitivity of Tobacco Protoplasts by Modifying the Intracellular Auxin Concentration

1994 ◽  
Vol 105 (2) ◽  
pp. 563-569 ◽  
Author(s):  
A. Delbarre ◽  
P. Muller ◽  
V. Imhoff ◽  
H. Barbier-Brygoo ◽  
C. Maurel ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Tobacco Protoplasts ◽  
Rolb Gene ◽  
Auxin Concentration

scholarly journals Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes

1995 ◽  
Vol 25 (2) ◽  
pp. 229-232
Author(s):  
Sergio Echeverrigaray
Keyword(s):  
Nicotiana Tabacum ◽  
Agrobacterium Rhizogenes ◽  
Biochemical Analysis ◽  
Electrophoretic Analysis ◽  
Plant Genome ◽  
Abnormal Development ◽  
Nicotiana Tabacum L ◽  
Sds Page ◽  
Auxin Concentration ◽  
High Auxin

Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the transformants due to the high auxin concentration codified by A. rhizogenes T-DNA incorporated to the plant genome.

Expression of Agrobacterium rhizogenes rolB gene fusions in Escherichia coli: production of antibodies against the RolB protein

Gene ◽  
1990 ◽  
Vol 87 (1) ◽  
pp. 139-143 ◽  
Author(s):  
M. Trovato ◽  
M. Cianfriglia ◽  
P. Filetici ◽  
M.L. Mauro ◽  
P. Costantino
Keyword(s):  
Escherichia Coli ◽  
Agrobacterium Rhizogenes ◽  
Gene Fusions ◽  
Rolb Gene

Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco

1990 ◽  
Vol 223 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Christophe Maurel ◽  
Jean Brevet ◽  
Hélène Barbier-Brygoo ◽  
Jean Guern ◽  
Jacques Tempé
Keyword(s):  
Transgenic Tobacco ◽  
Agrobacterium Rhizogenes ◽  
Rolb Gene

Effect of far-red light exposure on photosynthesis and photoprotection in tomato plants transgenic for the Agrobacterium rhizogenes rolB gene

2020 ◽  
Vol 245 ◽  
pp. 153095
Author(s):  
Priscilla P. Bettini ◽  
Luigi Lazzara ◽  
Luca Massi ◽  
Fabiola Fani ◽  
Maria Luisa Mauro
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Light Exposure ◽  
Red Light ◽  
Tomato Plants ◽  
Rolb Gene

Different promoter regions control level and tissue specificity of expression of Agrobacterium rhizogenes rolB gene in plants

1991 ◽  
Vol 16 (3) ◽  
pp. 427-436 ◽  
Author(s):  
I. Capone ◽  
M. Cardarelli ◽  
D. Mariotti ◽  
M. Pomponi ◽  
A. De Paolis ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Tissue Specificity ◽  
Control Level ◽  
Promoter Regions ◽  
Rolb Gene

The production of class III plant peroxidases in transgenic callus cultures transformed with the rolB gene of Agrobacterium rhizogenes

2013 ◽  
Vol 168 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Y.N. Shkryl ◽  
G.N. Veremeichik ◽  
V.P. Bulgakov ◽  
T.V. Avramenko ◽  
E.A. Günter ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Callus Cultures ◽  
Class Iii ◽  
Rolb Gene ◽  
Plant Peroxidases ◽  
Transgenic Callus

scholarly journals Effect of Different Strains of Agrobacterium rhizogenes and Nature of Explants on Plumbago indica Hairy Root Culture with Special Emphasis on Root Biomass and Plumbagin Production

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Moumita Gangopadhyay ◽  
Saikat Dewanjee ◽  
Somnath Bhattacharyya ◽  
Sabita Bhattacharya
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Root Biomass ◽  
Leaf Explants ◽  
Pcr Analysis ◽  
Stem Explants ◽  
Root Induction ◽  
Rolb Gene ◽  
Plumbago Indica

The aim of the present study was to determine the effect of three strains of Agrobacterium rhizogenes (ATCC 15834, A4 and LBA 9402) and the nature of explants (leaf and stem) on hairy root induction, growth and plumbagin production in Plumbago indica. The first appearance of hairy roots, the transformation frequency, dry root biomass and plumbagin accumulation were found to be maximum in hairy roots induced in leaf explants infected with A. rhizogenes ATCC 15834 as compared with the other two bacterial strains. The hairy roots generated from stem explants infected with all three strains were not found to be productive in terms of the selected parameters. Finally, the insertion of the rolB gene of A. rhizogenes ATCC 15834 in hairy roots of P. indica derived from leaf explants was confirmed by PCR analysis.

scholarly journals Involvement of Polyamides in Auxin and Agrobacterium rhizogenes - induced Rooting of Fruit Trees in Vitro

1992 ◽  
Vol 117 (3) ◽  
pp. 532-536 ◽  
Author(s):  
Eddo Rugini
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Fruit Trees ◽  
Prunus Dulcis ◽  
Malus Pumila ◽  
Positive Effects ◽  
Rooting Medium ◽  
Auxin Concentration ◽  
Positive Roots ◽  
Layer Chromatography

In the olive Olea europaea (L.), the polyamides (PAs), putrescine, spermidine, and spermine, when added exogenously at a concentration of 1 mm in the in vitro rooting medium, combined with 5 μm auxin concentration, promoted early rooting and increased the final rooting percentage and the number of roots per explant. The effect was less evident in olive explants rooted with the basal blanching method; thin-layer chromatography of total endogenous PAs in `these explants revealed lower levels on day 2 compared with controls, while by day 5 PA concentrations in both had fallen to similar levels. Furthermore, putrescine decreased the pH of the medium by 0.5 units around the explants. PAs had little effect on apple Malus pumila (Mill.) and no effect on almond Prunus dulcis (Miller) D.A. Webb and pistachio Pistacia vera (L.). There were also some positive effects observed, but only in olive, when rooting was induced by Agrobacterium rhizogenes in auxin-free medium. Few plantlets showed agropine-positive roots.

scholarly journals Hairy root induction in Catharanthus roseus by various strains of Agrobacterium rhizogenes isolated in Vietnam

2016 ◽  
Vol 19 (2) ◽  
pp. 44-52 ◽  
Author(s):  
Nhut Nhu Nguyen ◽  
Le Van Bui
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Root Production ◽  
Root Induction ◽  
Promising Tool ◽  
Enhanced Production ◽  
Rolb Gene ◽  
Polymerase Chain

Catharanthus roseus is a well known medicinal plant. It produces several phytocompounds and many of which show anticancerous properties. However the yields of these compounds are very low. Recently, induction of C. roseus hairy roots by Agrobacterium rhizogenes, is interested as a promising tool for the enhanced production of these metabolites. In this research, wounded leaves from four strains of C. roseus were infected with various strains of A. rhizogenes isolated in Vietnam to provide more information about the induction efficiency of hairy roots. In this experiment, after 3 weeks of infection, the presence of rolB gene in hairy roots were analysed by polymerase chain reaction. All of 13 A. rhizogenes strains could induce the formation of hairy root in C. roseus. The A. rhizogenes C18 strain had the highest induction percentage in C. roseus VIN002, VIN005, and VIN072 with 59.4 %, 50.3 %, and 40.0 % respectively. And the same result was ontained at 26.7% by A. Rhizogenes C26 for C. roseus VIN077 rhizogenes strains. This result identified two A. rhizogenes strains C18 and C26 as potential transformation tools for hairy root production from C. roseus.

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