scholarly journals Report on a project on three-dimensional imaging of the biological cell by single-particle X-ray diffraction. Addendum

Author(s):  
David A. Shapiro

In the paper by Sayre [Acta Cryst.(2008), A64, 33–35], a proposal is made to use stereoscopy as a short-term means of overcoming the primarily technological hurdles involved in three-dimensional imaging of the biological cell by soft X-ray diffraction microscopy. This addendum provides a broader perspective on the techniques used by this rapidly maturing community to investigate structural problems in the biological and material sciences.

Author(s):  
D. Sayre

Single-particle X-ray diffraction is an extension of X-ray crystallography which allows the specimen to be any small solid-state bounded object; in Shapiroet al.[Proc. Natl Acad. Sci. USA(2005),102, 15343–15346] and Thibaultet al.[Acta Cryst.(2006), A62, 248–261], the reader can find descriptions of a recent StonyBrook/Berkeley/Cornell two-dimensional imaging of a yeast cell by this technique. Our present work is aimed at extending the technique to the three-dimensional imaging of a cell. However, the usual method of doing that, namely rotating the specimen into many orientations in the X-ray beam, has not as yet given sufficiently good three-dimensional diffraction data to allow the work to go forward, the largest problem being the difficulty of preventing unwanted levels of change in the specimen through the extended exposure to a hostile environment of X-rays and, in some cases, high vacuum and/or extreme cold. The present paper discusses possible methods of dealing with this problem.


Materia Japan ◽  
2007 ◽  
Vol 46 (12) ◽  
pp. 827-827
Author(s):  
Yoshinori Nishino ◽  
Yukio Takahashi ◽  
Tetsuya Ishikawa ◽  
Eiichiro Matsubara

2012 ◽  
Vol 101 (24) ◽  
pp. 244103 ◽  
Author(s):  
D. Hänschke ◽  
L. Helfen ◽  
V. Altapova ◽  
A. Danilewsky ◽  
T. Baumbach

Nano Letters ◽  
2010 ◽  
Vol 10 (5) ◽  
pp. 1922-1926 ◽  
Author(s):  
Yukio Takahashi ◽  
Nobuyuki Zettsu ◽  
Yoshinori Nishino ◽  
Ryosuke Tsutsumi ◽  
Eiichiro Matsubara ◽  
...  

2003 ◽  
Vol 18 (2) ◽  
pp. 172-172
Author(s):  
W. Liu ◽  
G. E. Ice ◽  
W. Yang ◽  
J. Z. Tischler ◽  
B. C. Larson

2006 ◽  
Vol 23 (5) ◽  
pp. 1179 ◽  
Author(s):  
Henry N. Chapman ◽  
Anton Barty ◽  
Stefano Marchesini ◽  
Aleksandr Noy ◽  
Stefan P. Hau-Riege ◽  
...  

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