High-resolution structure of a plasmid-encoded dihydrofolate reductase: pentagonal network of water molecules in theD2-symmetric active site

2006 ◽  
Vol 62 (7) ◽  
pp. 695-706 ◽  
Author(s):  
Narendra Narayana
Keyword(s):  
High Resolution ◽  
Dihydrofolate Reductase ◽  
Active Site ◽  
Water Molecules ◽  
High Resolution Structure ◽  
Resolution Structure

scholarly journals High-resolution structure of an atypical α-phosphoglucomutase related to eukaryotic phosphomannomutases

2013 ◽  
Vol 69 (10) ◽  
pp. 2008-2016 ◽  
Author(s):  
Przemyslaw Nogly ◽  
Pedro M. Matias ◽  
Matteo de Rosa ◽  
Rute Castro ◽  
Helena Santos ◽  
...  
Keyword(s):  
Crystal Structure ◽  
High Resolution ◽  
Active Site ◽  
Catalytic Mechanism ◽  
Haloacid Dehalogenase ◽  
Enzyme Active Site ◽  
High Resolution Structure ◽  
Dimeric Form ◽  
Strict Specificity ◽  
Resolution Structure

The first structure of a bacterial α-phosphoglucomutase with an overall fold similar to eukaryotic phosphomannomutases is reported. Unlike most α-phosphoglucomutases within the α-D-phosphohexomutase superfamily, it belongs to subclass IIb of the haloacid dehalogenase superfamily (HADSF). It catalyzes the reversible conversion of α-glucose 1-phosphate to glucose 6-phosphate. The crystal structure of α-phosphoglucomutase fromLactococcus lactis(APGM) was determined at 1.5 Å resolution and contains a sulfate and a glycerol bound at the enzyme active site that partially mimic the substrate. A dimeric form of APGM is present in the crystal and in solution, an arrangement that may be functionally relevant. The catalytic mechanism of APGM and its strict specificity towards α-glucose 1-phosphate are discussed.

scholarly journals Dimeric and high-resolution structures of Chlamydomonas Photosystem I from a temperature-sensitive Photosystem II mutant

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Ido Caspy ◽  
Tom Schwartz ◽  
Vinzenz Bayro-Kaiser ◽  
Mariia Fadeeva ◽  
Amit Kessel ◽  
...  
Keyword(s):  
High Resolution ◽  
Photosystem Ii ◽  
Photosystem I ◽  
Water Molecules ◽  
Temperature Sensitive ◽  
Asymmetric Distribution ◽  
Light Harvesting Complexes ◽  
Transport Chain ◽  
High Resolution Structure ◽  
Resolution Structure

AbstractWater molecules play a pivotal functional role in photosynthesis, primarily as the substrate for Photosystem II (PSII). However, their importance and contribution to Photosystem I (PSI) activity remains obscure. Using a high-resolution cryogenic electron microscopy (cryo-EM) PSI structure from a Chlamydomonas reinhardtii temperature-sensitive photoautotrophic PSII mutant (TSP4), a conserved network of water molecules - dating back to cyanobacteria - was uncovered, mainly in the vicinity of the electron transport chain (ETC). The high-resolution structure illustrated that the water molecules served as a ligand in every chlorophyll that was missing a fifth magnesium coordination in the PSI core and in the light-harvesting complexes (LHC). The asymmetric distribution of the water molecules near the ETC branches modulated their electrostatic landscape, distinctly in the space between the quinones and FX. The data also disclosed the first observation of eukaryotic PSI oligomerisation through a low-resolution PSI dimer that was comprised of PSI-10LHC and PSI-8LHC.

scholarly journals High-resolution structure determination by continuous-rotation data collection in MicroED

2014 ◽  
Vol 11 (9) ◽  
pp. 927-930 ◽  
Author(s):  
Brent L Nannenga ◽  
Dan Shi ◽  
Andrew G W Leslie ◽  
Tamir Gonen
Keyword(s):  
High Resolution ◽  
Data Collection ◽  
Structure Determination ◽  
Continuous Rotation ◽  
High Resolution Structure ◽  
Resolution Structure

scholarly journals Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

FEBS Letters ◽  
2010 ◽  
Vol 584 (12) ◽  
pp. 2539-2547 ◽  
Author(s):  
Yo Sonoda ◽  
Alex Cameron ◽  
Simon Newstead ◽  
Hiroshi Omote ◽  
Yoshinori Moriyama ◽  
...  
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Structure Determination ◽  
High Resolution Structure ◽  
Resolution Structure
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