Crystallization and preliminary X-ray diffraction studies of cytochrome c 6 from Porphyra yezoensis

2000 ◽  
Vol 56 (1) ◽  
pp. 79-80
Author(s):  
Tomokazu Sasaki ◽  
Megumi Nakahara ◽  
Aya Matsuda ◽  
Takenobu Yamasaki ◽  
Sakae Katoh ◽  
...  
Keyword(s):  
Porphyra Yezoensis ◽  
Unit Cell ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Crystal Forms ◽  
Space Group ◽  
Cell Dimensions ◽  
Tetragonal Crystals ◽  
Tetragonal Form ◽  
Unit Cell Dimensions

Cytochrome c 6 from the red alga Porphyra yezoensis has been purified and crystallized by the sitting-drop vapour-diffusion method. Two different crystal forms, tetragonal and orthorhombic, were obtained. The tetragonal crystals belong to space group P41212 or P43212, with unit-cell dimensions a = 49.33 (2), c = 83.70 (10) Å. The orthorhombic crystals belong to space group P212121, with unit-cell dimensions a = 46.74 (2), b = 49.42 (1), c = 37.11 (1) Å. Absorption spectra of the crystals showed that the tetragonal form was oxidized and the orthorhombic form was reduced.

Crystallization and preliminary X-ray analysis of thiaminase I from Bacillus thiaminolyticus: space group change upon freezing of crystals

1998 ◽  
Vol 54 (3) ◽  
pp. 448-450 ◽  
Author(s):  
Nino Campobasso ◽  
Jakob Begun ◽  
Colleen A. Costello ◽  
Tadhg P. Begley ◽  
Steven E. Ealick
Keyword(s):  
Sodium Acetate ◽  
Crystal Form ◽  
Unit Cell ◽  
Diffusion Method ◽  
Crystal Forms ◽  
Space Group ◽  
X Ray ◽  
Cell Dimensions ◽  
Thiaminase I ◽  
Unit Cell Dimensions

Thiaminase I (Mr = 42 100) from B. thiaminolyticus, expressed in E. coli, has been crystallized by the vapor-diffusion method. Three crystal forms, two of which grew from 0.1 M sodium acetate (pH = 4.6), 0.2 M ammonium sulfate and 30%(w/v) PEG 2000, have been examined by X-ray analysis. One crystal form diffracted to 2.5 Å at room temperature, was orthorhombic, and had unit-cell edges of a = 87.7, b = 120.5 and c = 76.7 Å with space group P212121. A self-Patterson map showed a strong peak indicating noncrystallographic translational pseudosymmetry with (u, v, w) = (0.03, 0.0, 0.5). When these crystals were frozen at liquid-nitrogen temperatures, a second crystal form was observed which had unit-cell dimensions a = 85.5, b = 117.5 and c = 36.6 Å with space group P21212. A third crystal form grew from 0.1 M Tris (pH = 8.5), 0.2 M sodium acetate trihydrate and 28%(w/v) PEG 6000 to produce orthorhombic crystals of space group P212121 with cell edges of a = 114.4, b = 123.1 and c = 92.5 Å.

Crystallization and preliminary X-ray studies of the basic lectin from the seeds of Artocarpus hirsuta

1999 ◽  
Vol 55 (6) ◽  
pp. 1204-1205 ◽  
Author(s):  
K. N. Rao ◽  
Madhura M. Gurjar ◽  
Sushama M. Gaikwad ◽  
M. Islam Khan ◽  
C. G. Suresh
Keyword(s):  
Unit Cell ◽  
Diffusion Method ◽  
Hanging Drop ◽  
Crystal Forms ◽  
Space Group ◽  
X Ray ◽  
Hexagonal Form ◽  
Cell Dimensions ◽  
Crystallization Conditions ◽  
Unit Cell Dimensions

The basic lectin from Artocarpus hirsuta specific towards methyl α-galactose has been purified and crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitant. Three different crystal forms, orthorhombic I, orthorhombic II and hexagonal, were grown under the same crystallization conditions. The orthorhombic forms belonged to space group P212121 with unit-cell dimensions a = 92.9, b = 99.8, c = 166.2 Å and a = 89.9, b = 121.9, c = 131.6 Å, respectively. The unit-cell dimensions of the hexagonal form were a = b = 84.1 and c = 271.7 Å and the space group was P6122.

Synthesis and crystal structure of Na3.5Cr1.5Co0.5(PO4)3 phosphate

2006 ◽  
Vol 21 (3) ◽  
pp. 210-213 ◽  
Author(s):  
Mohamed Chakir ◽  
Abdelaziz El Jazouli ◽  
Jean-Pierre Chaminade
Keyword(s):  
Crystal Structure ◽  
Three Dimensional ◽  
Unit Cell ◽  
X Ray Diffraction ◽  
Synthesis And Crystal Structure ◽  
Space Group ◽  
X Ray ◽  
Hexagonal Unit Cell ◽  
Cell Dimensions ◽  
Unit Cell Dimensions

A new Nasicon phosphates series [Na3+xCr2−xCox(PO4)3(0⩽x⩽1)] was synthesized by a coprecipitation method and structurally characterized by powder X-ray diffraction. The selected compound Na3.5Cr1.5Co0.5(PO4)3 (x=0.5) crystallizes in the R3c space group with the following hexagonal unit-cell dimensions: ah=8.7285(3) Å, ch=21.580(2) Å, V=1423.8(1) Å3, and Z=6. This three-dimensional framework is built of PO4 tetrahedra and Cr∕CoO6 octahedra sharing corners. Na atoms occupy totally M(1) sites and partially M(2) sites.

Crystallization and preliminary X-ray diffraction analysis of malic enzyme from pigeon liver

1999 ◽  
Vol 55 (11) ◽  
pp. 1930-1932 ◽  
Author(s):  
Li-Chu Tsai ◽  
Chen-Chin Kuo ◽  
Wei-Yuan Chou ◽  
Gu-Gang Chang ◽  
Hanna S. Yuan
Keyword(s):  
Malic Enzyme ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Hanging Drop ◽  
Crystal Forms ◽  
X Ray ◽  
Cell Dimensions ◽  
Unit Cells ◽  
Unit Cell Dimensions ◽  
Pigeon Liver

Recombinant pigeon-liver malic enzyme was expressed in Escherichia coli and purified to homogeneity. Two different crystal forms were grown by the hanging-drop vapour-diffusion method. Both types of crystals belong to the tetragonal space group P4222, with unit-cell dimensions a = b = 163.8, c = 174.3 Å for the octahedral crystals and a = b = 124.5, c = 179.2 Å for the rod-like crystals. X-ray diffraction data were collected at 100 K using a synchrotron-radiation X-ray source. The Matthews parameter suggests that there are four and two molecules per asymmetric unit for the larger and the smaller tetragonal unit cells, respectively.

Crystallization and preliminary crystallographic analysis of winged bean acidic lectin

1999 ◽  
Vol 55 (2) ◽  
pp. 564-565 ◽  
Author(s):  
N. Manoj ◽  
V. R. Srinivas ◽  
B. Satish ◽  
Netai C. Singha ◽  
K. Suguna
Keyword(s):  
Crystallographic Analysis ◽  
Unit Cell ◽  
Winged Bean ◽  
Asymmetric Unit ◽  
Crystal Forms ◽  
Space Group ◽  
Replacement Method ◽  
Unit Form ◽  
Cell Dimensions ◽  
Unit Cell Dimensions

The acidic lectin (WBAII) from the winged bean (Psophocarpus tetragonolobus) binds to the H-antigenic determinant on human erythrocytes and to the T-antigenic disaccharide Gal-β1,3-GalNAc. Two crystal forms of WBAII were obtained in the presence of methyl-α-D-galactose. Form I belongs to space group R3 with unit-cell dimensions a = b = 182.11, c = 44.99 Å and has one dimer in the asymmetric unit. Form II belongs to space group C2 with unit-cell dimensions a = 135.36, b = 127.25, c = 139.98 Å, β = 95.9° and has four dimers in the asymmetric unit. Intensity data were collected to 3.0 Å and to 3.5 Å from crystals of form I and II, respectively. The structures were solved by the molecular-replacement method using the coordinates of the basic form of winged bean lectin.

Crystallization and preliminary X-ray studies on the hyperstable 3-isopropylmalate dehydrogenase from the thermoacidophilic archaeon Sulfolobus sp. strain 7

1998 ◽  
Vol 54 (3) ◽  
pp. 444-445
Author(s):  
Toshiharu Suzuki ◽  
Hideaki Moriyama ◽  
Raita Hirose ◽  
Masahiro Sakurai ◽  
Nobuo Tanaka ◽  
...  
Keyword(s):  
Ammonium Sulfate ◽  
Magnesium Chloride ◽  
Unit Cell ◽  
Diffusion Method ◽  
Orthorhombic System ◽  
Space Group ◽  
X Ray ◽  
Cell Dimensions ◽  
Unit Cell Dimensions ◽  
Isopropylmalate Dehydrogenase

3-Isopropylmalate dehydrogenase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7, has been crystallized by the vapor-diffusion method. The crystals were grown from a solution containing ammonium sulfate, 2-methyl-2,4-pentanediol and magnesium chloride. The crystallization requires 2-methyl-2,4-pentanediol to avoid twinning of the crystals. The crystal belongs to the orthorhombic system with the space group P2221 and unit-cell dimensions a = 67.9, b = 93.3 and c = 134.1 Å.

X-Ray Crystal Structure of [Rh(OH2)6](ClO4)3.3H2O

1989 ◽  
Vol 42 (11) ◽  
pp. 2051 ◽  
Author(s):  
GD Fallon ◽  
L Spiccia
Keyword(s):  
Crystal Structure ◽  
Single Crystal ◽  
Unit Cell ◽  
X Ray Diffraction ◽  
Space Group ◽  
X Ray ◽  
Octahedral Sites ◽  
Cell Dimensions ◽  
Unit Cell Dimensions

The crystal structure of [Rh(OH2)6](ClO4)3.3H2O has been determined by single-crystal X-ray diffraction and found to be isomorphous with that of M(ClO4)2.6H2O (M= Fe, Zn, Mn, Co, Ni) and LiClO4.3H2O. Crystal: are hexagonal, space group P63mc with unit cell dimensions a 7.817(2) and c 5.208(1) �. The lattice consists of a uniform arrangement of H2O and ClO4- groups with the RhIII centre occupying 1/3 of the octahedral sites formed by the H2O groups. The RhIII is not situated at the centre of the octahedron. However, the two Rh-O distances [2.128(6) and 2.136(6) �] may be considered identical, i.e. within the errors.

Purification, crystallization and preliminary X-ray diffraction studies on the thermostable catechol 2,3-dioxygenase of Bacillus stearothermophilus expressed in Escherichia coli

1998 ◽  
Vol 54 (3) ◽  
pp. 446-447 ◽  
Author(s):  
Min-Qin Chen ◽  
Chang-Chuan Yin ◽  
Wei Zhang ◽  
Yu-Min Mao ◽  
Zhi-Hong Zhang
Keyword(s):  
Escherichia Coli ◽  
Bacillus Stearothermophilus ◽  
Unit Cell ◽  
X Ray Diffraction ◽  
Data Set ◽  
Space Group ◽  
X Ray ◽  
Cell Dimensions ◽  
Unit Cell Dimensions

The thermostable catechol 2,3-dioxygenase of Bacillus stearothermophilus has been crystallized. The crystal is probably in the space group I 222 with unit-cell dimensions of a = 70.87, b = 74.60 and c = 133.69 Å. A native data set has been collected with a completeness of 96% at 2.22 Å resolution and an R merge value of 0.091.

scholarly journals Crystallization and X-ray diffraction analysis of native and selenomethionine-substituted PhyH-DI fromBacillussp. HJB17

2017 ◽  
Vol 73 (11) ◽  
pp. 607-611
Author(s):  
Fang Lu ◽  
Bei Zhang ◽  
Yong Liu ◽  
Ying Song ◽  
Gangxing Guo ◽  
...  
Keyword(s):  
Unit Cell ◽  
Diffusion Method ◽  
Data Sets ◽  
Asymmetric Unit ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
Solvent Content ◽  
Space Group ◽  
X Ray ◽  
Cell Parameters

Phytases are phosphatases that hydrolyze phytates to less phosphorylatedmyo-inositol derivatives and inorganic phosphate. β-Propeller phytases, which are very diverse phytases with improved thermostability that are active at neutral and alkaline pH and have absolute substrate specificity, are ideal substitutes for other commercial phytases. PhyH-DI, a β-propeller phytase fromBacillussp. HJB17, was found to act synergistically with other single-domain phytases and can increase their efficiency in the hydrolysis of phytate. Crystals of native and selenomethionine-substituted PhyH-DI were obtained using the vapour-diffusion method in a condition consisting of 0.2 Msodium chloride, 0.1 MTris pH 8.5, 25%(w/v) PEG 3350 at 289 K. X-ray diffraction data were collected to 3.00 and 2.70 Å resolution, respectively, at 100 K. Native PhyH-DI crystals belonged to space groupC121, with unit-cell parametersa = 156.84,b = 45.54,c = 97.64 Å, α = 90.00, β = 125.86, γ = 90.00°. The asymmetric unit contained two molecules of PhyH-DI, with a corresponding Matthews coefficient of 2.17 Å3 Da−1and a solvent content of 43.26%. Crystals of selenomethionine-substituted PhyH-DI belonged to space groupC2221, with unit-cell parametersa = 94.71,b= 97.03,c= 69.16 Å, α = β = γ = 90.00°. The asymmetric unit contained one molecule of the protein, with a corresponding Matthews coefficient of 2.44 Å3 Da−1and a solvent content of 49.64%. Initial phases for PhyH-DI were obtained from SeMet SAD data sets. These data will be useful for further studies of the structure–function relationship of PhyH-DI.

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