Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini fromDrosophila melanogaster
2012 ◽
Vol 68
(11)
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pp. 1346-1350
Keyword(s):
X Ray
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PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive.Drosophila melanogasterZuc (DmZuc) was expressed inEscherichia coli, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution. The crystal belonged to space groupP21, with unit-cell parametersa= 55.0,b= 71.2,c= 56.3 Å, β = 107.9°.
2012 ◽
Vol 68
(2)
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pp. 175-177
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2013 ◽
Vol 69
(4)
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pp. 421-424
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2015 ◽
Vol 71
(3)
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pp. 261-265
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1999 ◽
Vol 55
(9)
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pp. 1616-1617
2014 ◽
Vol 70
(9)
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pp. 1224-1227
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2012 ◽
Vol 68
(11)
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pp. 1351-1353
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2014 ◽
Vol 70
(11)
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pp. 1560-1562