scholarly journals Protein stability: a crystallographer's perspective

2016 ◽  
Vol 72 (2) ◽  
pp. 72-95 ◽  
Author(s):  
Marc C. Deller ◽  
Leopold Kong ◽  
Bernhard Rupp
Keyword(s):  
Protein Stability ◽  
Conformational Stability ◽  
Factors Affecting ◽  
Food Industries ◽  
Compositional Stability ◽  
Major Interest ◽  
Protein Conformational Stability ◽  
Academic Researchers ◽  
Stability Issues ◽  
Practical Level

Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.

scholarly journals Local water bridges and protein conformational stability

1999 ◽  
Vol 8 (10) ◽  
pp. 1982-1989 ◽  
Author(s):  
Michael Petukhov ◽  
David Cregut ◽  
Cláaaudio M. Soares ◽  
Luis Serrano
Keyword(s):  
Conformational Stability ◽  
Protein Conformational Stability ◽  
Local Water ◽  
Water Bridges

scholarly journals Conformational stability and dynamics in crystals recapitulate protein behaviour in solution

2020 ◽  
Author(s):  
BM Sala ◽  
T Le Marchand ◽  
G Pintacuda ◽  
C Camilloni ◽  
A Natalello ◽  
...  
Keyword(s):  
Protein Stability ◽  
Chemical Shifts ◽  
Conformational Stability ◽  
Conformational Dynamics ◽  
Protein Secondary Structure ◽  
Md Simulations ◽  
Thermal Stabilities ◽  
Crystalline Environment ◽  
Increasing Temperature ◽  
Hidden States

AbstractA growing body of evidences has established that in many cases proteins may preserve most of their function and flexibility in a crystalline environment, and several techniques are today capable to detect transiently-populated states of macromolecules in tightly packed lattices. Intriguingly, in the case of amyloidogenic precursors, the presence of these conformations (hidden to conventional crystallographic studies) can be correlated to the pathological fate of the native fold.It remains unclear, however, to which extent these minor conformations reflect the protein behaviour that is more commonly studied in solution. Here, we address this question by investigating some biophysical properties of a prototypical amyloidogenic system, β2-microglobulin (β2m) in solution and in microcrystalline state.By combining NMR chemical shifts with Molecular Dynamics (MD) simulations, we confirmed that conformational dynamics of β2m native state in the crystal lattice is in keeping with what observed in solution.A comparative study of protein stability in solution and in crystallo is then carried out, monitoring the change in protein secondary structure at increasing temperature by Fourier transform infrared (FTIR) spectroscopy. The increased structural order of the crystalline state contributes to provide better resolved spectral components compared to those collected in solution and crucially, the crystalline samples display thermal stabilities in good agreement with the trend observed in solution.Overall, this work shows that protein stability and occurrence of pathological hidden states in crystals parallel their solution counterpart, confirming the interest of crystals as a platform for the biophysical characterisation of processes such as unfolding and aggregation.

scholarly journals Sis1 potentiates the stress response to protein aggregation and elevated temperature

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Courtney L. Klaips ◽  
Michael H. M. Gropp ◽  
Mark S. Hipp ◽  
F. Ulrich Hartl
Keyword(s):  
Stress Response ◽  
Protein Aggregation ◽  
Protein Misfolding ◽  
Mammalian Cells ◽  
Environmental Changes ◽  
Conformational Stability ◽  
General Role ◽  
Heat Stress Response ◽  
Protein Conformational Stability ◽  
Polyq Protein

AbstractCells adapt to conditions that compromise protein conformational stability by activating various stress response pathways, but the mechanisms used in sensing misfolded proteins remain unclear. Moreover, aggregates of disease proteins often fail to induce a productive stress response. Here, using a yeast model of polyQ protein aggregation, we identified Sis1, an essential Hsp40 co-chaperone of Hsp70, as a critical sensor of proteotoxic stress. At elevated levels, Sis1 prevented the formation of dense polyQ inclusions and directed soluble polyQ oligomers towards the formation of permeable condensates. Hsp70 accumulated in a liquid-like state within this polyQ meshwork, resulting in a potent activation of the HSF1 dependent stress response. Sis1, and the homologous DnaJB6 in mammalian cells, also regulated the magnitude of the cellular heat stress response, suggesting a general role in sensing protein misfolding. Sis1/DnaJB6 functions as a limiting regulator to enable a dynamic stress response and avoid hypersensitivity to environmental changes.

Quality Control, Preparation, and Protein Stability Issues for Blood Serum and Plasma Used In Biomarker Discovery and Proteomic Profiling Assays

2004 ◽  
Vol 3 (4) ◽  
pp. 45-50 ◽  
Author(s):  
Richard Drake ◽  
Lisa Cazares ◽  
Alberto Corsica ◽  
Gunjan Malik ◽  
Ellen Schwegler ◽  
...  
Keyword(s):  
Quality Control ◽  
Blood Serum ◽  
Protein Stability ◽  
Biomarker Discovery ◽  
Proteomic Profiling ◽  
Stability Issues

INTEGRATED ADAPTIVE THRESHOLD BURST MODE RECEIVERS FOR OPTICAL DATA LINKS—AN ANALYSIS

1995 ◽  
Vol 06 (02) ◽  
pp. 375-394 ◽  
Author(s):  
ROBERT G. SWARTZ ◽  
YUSUKE OTA
Keyword(s):  
Steady State ◽  
Detailed Analysis ◽  
Dynamic Stability ◽  
Adaptive Threshold ◽  
Optical Data ◽  
Factors Affecting ◽  
Burst Mode ◽  
Data Links ◽  
Stability Issues ◽  
Factors Affecting Performance

This paper provides a detailed analysis of the principles of the adaptive threshold, burst mode receiver circuit, covering architectures for both current and voltage input modes. In it we derive the circuit’s fundamental operating equations, and look at steady-state and dynamic stability issues. The paper also describes techniques for active threshold reset, and reviews factors affecting performance of the circuit over temperature.

FOODS INGREDIENTS MANAGEMENT: INBOUND AND OUTBOUND HALAL MANAGEMENT BEST PRACTICE

2018 ◽  
Vol 13 (Number 2) ◽  
pp. 34-44
Author(s):  
Adam Mohd Saifudin ◽  
Matthew Lim Kai Teng ◽  
Tan Then Huei ◽  
Muhammad Adnin Naim Mohammad Yusof ◽  
Che Noraini Che Hussin ◽  
...  
Keyword(s):  
Cold Storage ◽  
Best Practice ◽  
Operational Decisions ◽  
Food Ingredients ◽  
Factors Affecting ◽  
Future Studies ◽  
Food Industries ◽  
Independent Variables ◽  
Halal Food

Foods and ingredients management in the inbound and outbound of warehouse has become significant priority to ensure the quality of halal products. This study will benefit the society among the Muslims and Non-Muslims communities. Manufacturers can interconnect the information to improve and identify factors affecting the consumers who are doubtful of choosing halal products. The objective of the study focus to examine the inbound and outbound efficiency of food ingredients management for halal management best practice in the warehouse. The researchers chose Saudi Cold Storage Sdn. Bhd as the model and respondents of the study. Questionnaires consisted of 26 questions are applied and distributed to the company’s employees. Inbound and outbound efficiency of food ingredients management are the independent variables for halal management best practice who acted as dependent variable. Theoretically, this research contributed the study on the inbound and outbound efficiencies of food ingredients management affectedthe halal management best practice. Practically, the study is applicable for managers in the halal food industries in their operational decisions making. The outcome of this research is positively reacted and explored for future studies related to halal warehousing and its operations.

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