Applying Time-Lapse Concepts onto Storage System for Long-Term System Trace Analysis: Technical Challenges and Blueprints

2018 ◽  
Author(s):  
Ki-Woong Park ◽  
Daeseon Choi ◽  
Woo-Jin Jeon
Keyword(s):  
Trace Analysis ◽  
Storage System ◽  
Time Lapse ◽  
Technical Challenges

scholarly journals Fertility and Pregnancy in End Stage Kidney Failure Patients and after Renal Transplantation: An Update

2021 ◽  
Vol 2 (2) ◽  
pp. 92-108
Author(s):  
Maurizio Salvadori ◽  
Aris Tsalouchos
Keyword(s):  
Renal Transplantation ◽  
Time Lapse ◽  
Sexual Life ◽  
End Stage Kidney Disease ◽  
Correct Evaluation ◽  
Functional Aspects ◽  
Long Term Follow Up ◽  
End Stage

Sexual life and fertility are compromised in end stage kidney disease both in men and in women. Successful renal transplantation may rapidly recover fertility in the vast majority of patients. Pregnancy modifies anatomical and functional aspects in the kidney and represents a risk of sensitization that may cause acute rejection. Independently from the risks for the graft, pregnancy in kidney transplant may cause preeclampsia, gestational diabetes, preterm delivery, and low birth weight. The nephrologist has a fundamental role in correct counseling, in a correct evaluation of the mother conditions, and in establishing a correct time lapse between transplantation and conception. Additionally, careful attention must be given to the antirejection therapy, avoiding drugs that could be dangerous to the newborn. Due to the possibility of medical complications during pregnancy, a correct follow-up should be exerted. Even if pregnancy in transplant is considered a high risk one, several data and studies document that in the majority of patients, the long-term follow-up and outcomes for the graft may be similar to that of non-pregnant women.

scholarly journals Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han
Keyword(s):  
Drug Treatment ◽  
Single Cell ◽  
Mycobacterium Smegmatis ◽  
Culture Media ◽  
Low Cost ◽  
Bacterial Pathogen ◽  
Growth Dynamics ◽  
Time Lapse ◽  
Cell Phenotype

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.

scholarly journals Long-Term Hypoxic/Hypocapnic Storage of Red Blood Cells Results in Amelioration of Lesion Hallmarks and Increased In Vivorecovery at 24 Hours Post-Transfusion

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 4995-4995
Author(s):  
Jose A Cancelas ◽  
F. Bernadette West ◽  
Matthew Karaffin ◽  
Tatsuro Yoshida ◽  
Neeta Rugg ◽  
...  
Keyword(s):  
Shelf Life ◽  
Research Funding ◽  
Storage System ◽  
Processing System ◽  
Test Group ◽  
Wilcoxon Test ◽  
And Control ◽  
2,3 Dpg

Oxidative damage, dysmetabolism and decreased levels of 2, 3-diphosphoglycerate (2,3-DPG) affect red blood cell (RBC) survival and the affinity of Hemoglobin for oxygen are relevant hallmarks of RBC storage lesion, especially in specific clinical contexts. We hypothesized that oxygen and carbon dioxide reduction followed by hypoxic/hypocapnic storage would result in amelioration of RBC lesion assessed at the end of their shelf-life and at the shelf-life mode (21 days).To determine the relevance of hypoxic/hypocapnic storage in humans, a pivotal, prospective, randomized, two‐arm, crossover, three‐center trial was conducted to evaluate [Hemanext®] hypoxic/hypocapnic processing system and was used to assess whether this process and storage method met standard FDA acceptability criteria for long-term RBC storage. The Test arm consisted of whole blood (WB)‐derived, leukoreduced RBC in AS-3 additive processed at room temperature with the Hemanext system for 3 hours to achieve hypoxic/hypocapnic state within 12 hours of phlebotomy which was maintained hypoxic during storage for up to 43 days at 1‐6°C (Test).Unprocessed units (Control) were stored within 8 hours under conventional storage conditions. Subjects (N=100) donated CP2D WB (500 ± 50 mL) and a minimum of 93 pairs of RBC units per arm were analyzed for in vitroquality parameters. For in vivo analysis at end of storage, RBCs from 19 test subjects and 21 control subjects (14 paired) from two sites were radiolabeled with 51‐Cr/99‐Tc(m), autologously transfused, and analyzed for 24-hour recovery. Differences between the Test and Control groups were analyzed using the paired t-test (Wilcoxon test where necessary). Paired analyses of 24-hour in vivorecoveries on day 43 was 89.3±4.5% and 84.8±6.2% for the test and control, respectively; p<0.01). Significantly higher levels of 2,3-DPG and adenosine 5'-triphosphate (ATP) were maintained for the Test group by days 21 and 42/43 of storage (Table 1). Percentage of hemolysis was similar in both groups. In summary, these data demonstrate that RBCs preserved in a user-friendly, self-contained hypoxic storage system are superior than the conventionally stored RBCs and may provide more viable RBCs for transfusion at 6 weeks of storage. Disclaimer: The51‐Cr/99‐Tc(m)labeling at one of the three sites occurred without prior RDRC approval and did not meet GMP standards. Disclosures Cancelas: Fresenius-Kabi: Research Funding; Cerus Co.: Research Funding; TerumoBCT: Consultancy, Research Funding; Hemanext: Consultancy, Research Funding; Macopharma Inc: Research Funding; Cytosorbents: Research Funding; Cellphire: Research Funding; Velico: Consultancy, Research Funding. Yoshida:New Health Sciences Inc.: Employment, Equity Ownership. Dioguardi:Hemanext: Employment. Iselin:Hemanext: Employment. Dunham:Hemanext: Employment.

scholarly journals A Self-contained and Self-explanatory DNA Storage System

2021 ◽  
Author(s):  
Min Li ◽  
Junbiao Dai ◽  
Qingshan Jiang ◽  
Yang Wang
Keyword(s):  
Data Storage ◽  
Storage System ◽  
Data File ◽  
Data Redundancy ◽  
Dna Storage ◽  
Data Restoration ◽  
Gene Data ◽  
High Uncertainty ◽  
Term Data

Abstract Current research on DNA storage usually focuses on the improvement of storage density with reduced gene synthesis cost by developing effective encoding and decoding schemes while lacking the consideration on the uncertainty in ultra long-term data storage and retention. Consequently, the current DNA storage systems are often not self-containment, implying that they have to resort to external tools for the restoration of the stored gene data. This may result in high risks in data loss since the required tools might not be available due to the high uncertainty in far future. To address this issue, we propose in this paper a self-contained DNA storage system that can make self-explanatory to its stored data without relying on any external tools. To this end, we design a specific DNA file format whereby a separate storage scheme is developed to reduce the data redundancy while an effective indexing is designed for random read operations to the stored data file. We verified through experimental data that the proposed self-contained and self-explanatory method can not only get rid of the reliance on external tools for data restoration but also minimize the data redundancy brought about when the amount of data to be stored reaches a certain scale.

scholarly journals Long-term time-lapse microgravity and geotechnical monitoring of relict salt mines, Marston, Cheshire, U. K.

Geophysics ◽  
2012 ◽  
Vol 77 (6) ◽  
pp. B287-B294 ◽  
Author(s):  
Jamie K. Pringle ◽  
Peter Styles ◽  
Claire P. Howell ◽  
Michael W. Branston ◽  
Rebecca Furner ◽  
...  
Keyword(s):  
Time Lapse ◽  
Ground Subsidence ◽  
Near Surface ◽  
Geotechnical Monitoring ◽  
Salt Mines ◽  
History Of ◽  
Mine Workings ◽  
The Village ◽  
The Town

The area around the town of Northwich in Cheshire, U. K., has a long history of catastrophic ground subsidence caused by a combination of natural dissolution and collapsing abandoned mine workings within the underlying Triassic halite bedrock geology. In the village of Marston, the Trent and Mersey Canal crosses several abandoned salt mine workings and previously subsiding areas, the canal being breached by a catastrophic subsidence event in 1953. This canal section is the focus of a long-term monitoring study by conventional geotechnical topographic and microgravity surveys. Results of 20 years of topographic time-lapse surveys indicate specific areas of local subsidence that could not be predicted by available site and mine abandonment plan and shaft data. Subsidence has subsequently necessitated four phases of temporary canal bank remediation. Ten years of microgravity time-lapse data have recorded major deepening negative anomalies in specific sections that correlate with topographic data. Gravity 2D modeling using available site data found upwardly propagating voids, and associated collapse material produced a good match with observed microgravity data. Intrusive investigations have confirmed a void at the major anomaly. The advantages of undertaking such long-term studies for near-surface geophysicists, geotechnical engineers, and researchers working in other application areas are discussed.

Value Creation by a Long-term Time-lapse Processing Approach on the Heidrun Field

2012 ◽  
Author(s):  
D. Fischer ◽  
P. Smith ◽  
N. Sørenes ◽  
E. Teichmann ◽  
H. Blekastad ◽  
...  
Keyword(s):  
Value Creation ◽  
Time Lapse ◽  
Processing Approach

scholarly journals Temperature Measurements on a Solar and Low Enthalpy Geothermal Open-Air Asphalt Surface Platform in a Cold Climate Region

Energies ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 979 ◽  
Author(s):  
Caner Çuhac ◽  
Anne Mäkiranta ◽  
Petri Välisuo ◽  
Erkki Hiltunen ◽  
Mohammed Elmusrati
Keyword(s):  
Heat Flux ◽  
Urban Areas ◽  
Storage System ◽  
Energy Resource ◽  
Cold Climate ◽  
Solar Irradiation ◽  
Solar Heat ◽  
Heat Collector ◽  
One Year

Solar heat, already captured by vast asphalt fields in urban areas, is potentially a huge energy resource. The vertical soil temperature profile, i.e., low enthalpy geothermal energy, reveals how efficiently the irradiation is absorbed or radiated back to the atmosphere. Measured solar irradiation, heat flux on the asphalt surface and temperature distribution over a range of depths describe the thermal energy from an asphalt surface down to 10 m depth. In this study, those variables were studied by long-term measurements in an open-air platform in Finland. To compensate the nighttime heat loss, the accumulated heat on the surface should be harvested during the sunny daytime periods. A cumulative heat flux over one year from asphalt to the ground was 70% of the cumulative solar irradiance measured during the same period. However, due to the nighttime heat losses, the net heat flux during 5 day period was only 18% of the irradiance in spring, and was negative during autumn, when the soil was cooling. These preliminary results indicate that certain adaptive heat transfer and storage mechanisms are needed to minimize the loss and turn the asphalt layer into an efficient solar heat collector connected with a seasonal storage system.

Global Assessment System for Structural Safety in Large Size Storage Tanks

2004 ◽  
Author(s):  
Shinichi Kaita ◽  
Toshikazu Shibasaki ◽  
Takayasu Tahara
Keyword(s):  
Crude Oil ◽  
Global Assessment ◽  
Structural Integrity ◽  
Storage System ◽  
Assessment System ◽  
Structural Safety ◽  
Risk Level ◽  
Storage Tanks ◽  
Large Size

Considering long term stable supply of oil fuel, the world largest long-term storage system of crude oil has been installed in Japan. In order to ensure safety of large size above ground storage tanks, global assessment system for structural integrity of tank considering risk level and shut down inspection interval has been developed on Risk Based Inspection, RBI and Fitness-For-Service, FFS for storage tanks of crude oil for national security reserve.

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