In vitro evaluation of red blood cell flow in bifurcating microchannel

SummaryThe results of thyroid function tests have been compared with data on the thyroxine-binding capacity of plasma proteins in hyper-, hypo- and euthyroid cases, the latter including women taking oral contraceptives (Infecundin). It was found that there exists a significant correlation of exponential nature between the in vitro red blood cell 125I-triiodothyronine uptake (RCU) and the free thyroxine-binding capacity of the thyroxine-inding globulin (TBG).

Download Full-text

Human Red Blood Cell (Hrbc) Membrane Stabilization Potential of Crude Methanolic Leaf Extract of Gymnema Sylvestre - An In Vitro Study

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.01.381 ◽

2020 ◽

Vol 12 (01) ◽

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Leaf Extract ◽

In Vitro Study ◽

Vitro Study ◽

Gymnema Sylvestre ◽

Membrane Stabilization

Download Full-text

Patents on Red Blood Cell Manufacturing In Vitro

Recent Patents on Regenerative Medicine ◽

10.2174/2210297311101020173 ◽

2011 ◽

Vol 1 (2) ◽

pp. 173-181

Author(s):

Laurence Guyonneau-Harmand ◽

Luc Douay

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Cell Manufacturing

Download Full-text

In vitro effects of temperature on red blood cell deformability and membrane stability in human and various vertebrate species

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-211118 ◽

2021 ◽

pp. 1-10

Author(s):

Adam Attila Matrai ◽

Gabor Varga ◽

Bence Tanczos ◽

Barbara Barath ◽

Adam Varga ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Whole Blood ◽

Mechanical Stability ◽

Cell Deformability ◽

Blood Samples ◽

Effects Of Temperature ◽

The Way ◽

Rbc Deformability

BACKGROUND: The effects of temperature on micro-rheological variables have not been completely revealed yet. OBJECTIVE: To investigate micro-rheological effects of heat treatment in human, rat, dog, and porcine blood samples. METHODS: Red blood cell (RBC) - buffer suspensions were prepared and immersed in a 37, 40, and 43°C heat-controlled water bath for 10 minutes. Deformability, as well as mechanical stability of RBCs were measured in ektacytometer. These tests were also examined in whole blood samples at various temperatures, gradually between 37 and 45°C in the ektacytometer. RESULTS: RBC deformability significantly worsened in the samples treated at 40 and 43°C degrees, more expressed in human, porcine, rat, and in smaller degree in canine samples. The way of heating (incubation vs. ektacytometer temperation) and the composition of the sample (RBC-PBS suspension or whole blood) resulted in the different magnitude of RBC deformability deterioration. Heating affected RBC membrane (mechanical) stability, showing controversial alterations. CONCLUSION: Significant changes occur in RBC deformability by increasing temperature, showing inter-species differences. The magnitude of alterations is depending on the way of heating and the composition of the sample. The results may contribute to better understanding the micro-rheological deterioration in hyperthermia or fever.

Download Full-text

Absence of the Adaptor Protein PEA-15 Is Associated with Altered Pattern of Th Cytokines Production by Activated CD4+ T Lymphocytes In Vitro, and Defective Red Blood Cell Alloimmune Response In Vivo

PLoS ONE ◽

10.1371/journal.pone.0136885 ◽

2015 ◽

Vol 10 (8) ◽

pp. e0136885 ◽

Cited By ~ 6

Author(s):

Stéphane Kerbrat ◽

Benoit Vingert ◽

Marie-Pierre Junier ◽

Flavia Castellano ◽

François Renault-Mihara ◽

...

Keyword(s):

T Lymphocytes ◽

Blood Cell ◽

Red Blood Cell ◽

Adaptor Protein

Download Full-text

Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1

Transfusion ◽

10.1111/trf.13138 ◽

2015 ◽

Vol 55 (9) ◽

pp. 2197-2206 ◽

Cited By ~ 12

Author(s):

Diana Mittag ◽

Amrita Sran ◽

Kasey S. Chan ◽

Martin P. Boland ◽

Esther Bandala-Sanchez ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Stress Conditions

Download Full-text

Desensitization of Adrenaline-Induced Red Blood Cell H+ Extrusion in Vitro After Chronic Exposure of Rainbow Trout to Moderate Environmental Hypoxia

Journal of Experimental Biology ◽

10.1242/jeb.156.1.233 ◽

1991 ◽

Vol 156 (1) ◽

pp. 233-248 ◽

Cited By ~ 1

Author(s):

S. THOMAS ◽

R. KINKEAD ◽

P. J. WALSH ◽

C. M. WOOD ◽

S. F. PERRY

Keyword(s):

Rainbow Trout ◽

Blood Cell ◽

Red Blood Cell ◽

Whole Blood ◽

Inverse Correlation ◽

Adrenergic Stimulation ◽

Plasma Adrenaline ◽

Arterial Blood Gas ◽

Arterial Blood

The sensitivity of red blood cell Na+/H+ exchange to exogenous adrenaline was assessed in vitro using blood withdrawn from catheterized rainbow trout (Oncorhynchus mykiss) maintained under normoxic conditions [water PO2, (PwO2)=20.66 kPa] or after exposure to moderate hypoxia (PwO2=6.67-9.33 kPa) for 48 h, which chronically elevated plasma adrenaline, but not noradrenaline, levels. Peak changes in whole-blood extracellular pH over a 30 min period after adding 50–1000 nmoll−1 adrenaline were employed as an index of sensitivity; the blood was pre-equilibrated to simulate arterial blood gas tensions in severely hypoxic fish (PaO2=2.0 kPa, PaCO2=0.31 kPa). Blood pooled from normoxic fish displayed a dose-dependent reduction in whole-blood pH after addition of adrenaline. Blood pooled from three separate groups of hypoxic fish, however, displayed diminished sensitivity to adrenaline, ranging from complete desensitization to a 60%reduction of the response. Subsequent experiments performed on blood from individual (i.e. not pooled) normoxic or hypoxic fish demonstrated an inverse correlation between the intensity of H+ extrusion (induced by exogenous adrenaline addition) and endogenous plasma adrenaline levels at the time of blood withdrawal. However, acute increases in plasma adrenaline levels in vitro did not affect the responsiveness of the red blood cell to subsequent adrenergic stimulation. The intensity of H+ extrusion was inversely related to the PaO2in vivo between 2.67 and 10.66 kPa, and directly related to the logarithm of the endogenous plasma adrenaline level. The results suggest that desensitization of Na+/H+ exchange in chronically hypoxic fish is related to persistent elevation of levels of this catecholamine. This desensitization can be reversed in vitro as a function of time, but only when blood is maintained under sufficiently aerobic conditions.

Download Full-text