scholarly journals An in vitro evaluation of leukocyte-poor red blood cell concentrates prepared by reconstitution with phosphate buffer solution.

1983 ◽  
Vol 139 (4) ◽  
pp. 417-418 ◽  
Author(s):  
TETSUO SHIMIZU ◽  
YASUO MORISHIMA ◽  
SHOJI GOTO ◽  
GANZO HASEGAWA ◽  
TSUNEO FUKUDA
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
In Vitro Evaluation

In vitro evaluation of di(2-ethylhexyl)terephthalate-plasticized polyvinyl chloride blood bags for red blood cell storage in AS-1 and PAGGSM additive solutions

Transfusion ◽  
2018 ◽  
Vol 58 (5) ◽  
pp. 1100-1107 ◽  
Author(s):  
Sharon Graminske ◽  
Kathleen Puca ◽  
Anna Schmidt ◽  
Scott Brooks ◽  
Amanda Boerner ◽  
...  
Keyword(s):  
Blood Cell ◽  
Polyvinyl Chloride ◽  
Red Blood Cell ◽  
In Vitro Evaluation ◽  
Cell Storage

scholarly journals Encapsulation of Polyphenols from Lycium barbarum Leaves into Liposomes as a Strategy to Improve Their Delivery

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1938
Author(s):  
Ramona-Daniela Păvăloiu ◽  
Fawzia Sha’at ◽  
Georgeta Neagu ◽  
Mihaela Deaconu ◽  
Corina Bubueanu ◽  
...  
Keyword(s):  
Particle Size ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Entrapment Efficiency ◽  
Cytoprotective Effect ◽  
Buffer Solution ◽  
Lycium Barbarum ◽  
Burst Effect ◽  
Pharmaceutical Applications

This study is focused on the encapsulation of polyphenols from Lycium barbarum leaves into liposomes as a strategy to improve their delivery. Liposomes loaded with Lycium barbarum leaves extract were obtained and characterized for particle size, polydispersity, entrapment efficiency, and stability. Liposomes presented entrapment efficiency higher than 75%, nanometric particle size, narrow polydispersity, and good stability over three months at 4 °C. The liposomes containing Lycium barbarum offered a slower release of polyphenols with attenuated burst effect compared with the dissolution of free Lycium barbarum extract in phosphate buffer solution at pH 7.4. Moreover, an in vitro pretreatment of 24 h with loaded liposomes showed a cytoprotective effect against H2O2-induced cytotoxicity on L-929 mouse fibroblasts cells. These preliminary findings imply that liposomes could be successfully employed as carriers for polyphenols in pharmaceutical applications.

Preparation and Characterization of Danazol Nanoparticles for Dissolution Improvement

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Luma Safa el-din Al-Hassnaui
Keyword(s):  
Particle Size ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Amorphous State ◽  
Volume Ratio ◽  
In Vitro Dissolution ◽  
Buffer Solution ◽  
Solution Ph ◽  
Freeze Dried

Danazol is a synthetic steroid used for endometriosis treatment, haslow bioavailability as it is practically insoluble in water. This study has been carried out to prepare and characterize danazol nanoparticles by nanoprecipitation method at a different polymer to drug ratios of 0.5:1,1:1,2:1 and 3:1 using different polymers of CMC-30 and various grades of HPMC and PVP,as stabilizers. Variables that might affect the particle size as polymer type,polymer to drug ratio,temperature of precipitation,addition rate of danazol solution,volume ratio,time of stirring,concentrationof drug,have been investigated. The particle size of the prepared formulas has been in the nano-sized except those using CMC and the best formula has beenF20 at a polymer to drug ratio of0.5:1 which has given the smallest particle sizeof 33nm.The investigations of the drug–stabilizer compatibility havebeen studied by FTIR and DSC,crystalline state by XRD,size,and shape of nanoparticles by FESEM and the results showed that there has been no interaction between the danazol and stabilizer and there has been a partial conversion of danazol from crystalline to an amorphous state with a size below 100nm. Most of the studied factors havebeen found affect the particle size of the nanoparticles.The Entrapment efficiency has been (91.3% ± 0.4) in the (F20). The solubility study revealed that 6.75,4.97 and 5.1 folds increased of solubility of danazol for nanoparticles than that for raw in distilled water,0.1N HCl and in phosphate buffer of pH 6.8.The simple capsule has been prepared by incorporation of freeze-dried of F20 with lactose as a filler and the in vitro dissolution study has been conducted using 0.1N HCl (pH 1.2) with 2% w/v Brij-35,phosphate buffer solution(pH 6.8) with 2% w/v Brij-35as dissolution media. Within 30 minutes,100% of the danazol has been released from the nanoparticle capsule in both dissolution media compared to the raw and physical blend capsules as controls havebeen nearly complete in 120 minutes.One can conclude that Antisolvent method is an easy,efficient method to prepare danazol nanoparticles with an intense effect on solubility and faster in vitro dissolution rate than raw drug and its physical blend with stabilizer.

The Effect of Plasma Treatment on the Performance of Poly(lactide-Co-Glycolide) Fiber

2011 ◽  
Vol 295-297 ◽  
pp. 103-106
Author(s):  
Zheng Guo ◽  
Jin Jing Chen
Keyword(s):  
Contact Angle ◽  
Tensile Strength ◽  
Plasma Treatment ◽  
Rough Surface ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Untreated Fiber

In this paper, Poly(lactide-co-glycolide) (PGLA) fibers were treated with the plasma. The surface, tensile strength, and contact angle with water of the PGLA fiber were investigated. The results showed that the PGLA fiber after treatment possessed more rough surface and smaller contact angle with water compared with the untreated fiber, but the tensile strength of fiber after treatment was not significantly diffrent form the fiber before treatment. In addition, both the treated and untreated PGA fibers were placed in phosphate buffer solution (PBS) (pH=7.4) at 37 °C up to 2.5 weeks to investigate the effect of plasma treatment on the degradation in vitro. Changes in tensile strength during degradation was investigated. The results showed that the treated PGLA fiber degraded faster compared with the untreated fiber.

Preparation and Properties of Thermosensitive 5-Fu-ACS Hydrogel

2011 ◽  
Vol 236-238 ◽  
pp. 2387-2390
Author(s):  
He Ping Li ◽  
Tao Yu ◽  
Shao Peng Liu ◽  
Shan Li ◽  
Long Qin
Keyword(s):  
Phosphate Buffer ◽  
Drug Carrier ◽  
Phosphate Buffer Solution ◽  
Control Release ◽  
Uv Spectrum ◽  
Buffer Solution ◽  
Release Drug

In the article, a new thermosensitive 5-Fu-ACS hydrogel was prepared as control release drug carrier, and its in vitro release capability in phosphate buffer solution (PBS) were determined by UV spectrum

scholarly journals In Vitro Structural Changes of Nano-Bacterial Cellulose Immersed in Phosphate Buffer Solution

2011 ◽  
Vol 10 ◽  
pp. 55-66 ◽  
Author(s):  
Yan Mei Chen ◽  
Ting Fei Xi ◽  
Yu Feng Zheng ◽  
Liang Zhou ◽  
Yi Zao Wan
Keyword(s):  
Bacterial Cellulose ◽  
Phosphate Buffer ◽  
Structural Changes ◽  
Ph Value ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Potential Applications ◽  
Scaffold Materials

Nano-bacterial cellulose (nBC), secreted by Acetobacter xylinum, is expected to have potential applications in tissue engineering. In this paper, the in-vitro degradation performance and the corresponding mechanism of nBC immersed in phosphate buffer solution (PBS) for different time periods was investigated. The pH value variation of solution, material degradation, and the swelling and structural changes of nBC was analysed successively. The results indicate that water molecules attack the exposed nBC fibrils, weakening the bonding strength of inter- and intra-molecular chains and disconnecting partial C-O-C bonds. The disconnection of C-O-C bonds is considered the primary reason for the degradation of nBC large molecular chains after nBC is immersed in PBS. The present work is instructive for controlling the in-vivo degradation performance of nBC acting as bone tissue engineered scaffold materials.

Investigation of the Free and Total Thyroxine-binding Capacity of Plasma Proteins in Thyroid Disorders

1971 ◽  
Vol 10 (04) ◽  
pp. 299-304
Author(s):  
József Takó ◽  
János Fischer ◽  
Jusztina Juhász ◽  
Ilona Sztraka ◽  
István Kapus ◽  
...  
Keyword(s):  
Blood Cell ◽  
Thyroid Function ◽  
Oral Contraceptives ◽  
Red Blood Cell ◽  
Plasma Proteins ◽  
Binding Capacity ◽  
Thyroid Disorders ◽  
Thyroid Function Tests ◽  
Total Thyroxine

SummaryThe results of thyroid function tests have been compared with data on the thyroxine-binding capacity of plasma proteins in hyper-, hypo- and euthyroid cases, the latter including women taking oral contraceptives (Infecundin). It was found that there exists a significant correlation of exponential nature between the in vitro red blood cell 125I-triiodothyronine uptake (RCU) and the free thyroxine-binding capacity of the thyroxine-inding globulin (TBG).

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