Application of Electrically Charged Carbon Nanodots@glass in DNA and Cell Engineering

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both in vitro and in vivo cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for in vivo self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by in vitro self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

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Structure–Activity Relationships and Gas Diffusion Cell Engineering for CO2 and CO Electrolysis

10.29363/nanoge.ngfm.2019.064 ◽

2019 ◽

Author(s):

Matthew Kanan

Keyword(s):

Gas Diffusion ◽

Cell Engineering ◽

Diffusion Cell ◽

Structure Activity Relationships ◽

Structure Activity

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Faculty Opinions recommendation of CellNet: network biology applied to stem cell engineering.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718533390.793508481 ◽

2015 ◽

Author(s):

Timm Schroeder ◽

Stavroula Skylaki

Keyword(s):

Stem Cell ◽

Network Biology ◽

Cell Engineering ◽

Stem Cell Engineering

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The modulation of the photophysical and photodynamic therapy activities of a phthalocyanine by detonation nanodiamonds: Comparison with graphene quantum dots and carbon nanodots

Diamond and Related Materials ◽

10.1016/j.diamond.2019.107617 ◽

2020 ◽

Vol 101 ◽

pp. 107617 ◽

Cited By ~ 4

Author(s):

Refilwe Matshitse ◽

Muthumuni Managa ◽

Tebello Nyokong

Keyword(s):

Quantum Dots ◽

Photodynamic Therapy ◽

Graphene Quantum Dots ◽

Detonation Nanodiamonds ◽

Carbon Nanodots

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Usefulness of collaboration between mathematical models and cell engineering for elucidating complex disease mechanisms and discover effective drugs

European Heart Journal ◽

10.1093/ehjci/ehaa946.3600 ◽

2020 ◽

Vol 41 (Supplement_2) ◽

Author(s):

H Kohjitani ◽

A Kashiwa ◽

T Makiyama ◽

F Toyoda ◽

Y Yamamoto ◽

...

Keyword(s):

Mathematical Model ◽

In Silico ◽

Complex Disease ◽

Ion Selectivity ◽

Public Institution ◽

Cell Engineering ◽

Funding Source ◽

Patch Clamp Technique ◽

In Silico Model

Abstract Background A missense mutation, CACNA1C-E1115K, located in the cardiac L-type calcium channel (LTCC), was recently reported to be associated with diverse arrhythmias. Several studies reported in-vivo and in-vitro modeling of this mutation, but actual mechanism and target drug of this disease has not been clarified due to its complex ion-mechanisms. Objective To reveal the mechanism of this diverse arrhythmogenic phenotype using combination of in-vitro and in-silico model. Methods and results Cell-Engineering Phase: We generated human induced pluripotent stem cell (hiPSC) from a patient carrying heterozygous CACNA1C-E1115K and differentiated into cardiomyocytes. Spontaneous APs were recorded from spontaneously beating single cardiomyocytes by using the perforated patch-clamp technique. Mathematical-Modeling Phase: We newly developed ICaL-mutation mathematical model, fitted into experimental data, including its impaired ion selectivity. Furthermore, we installed this mathematical model into hiPSC-CM simulation model. Collaboration Phase: Mutant in-silico model showed APD prolongation and frequent early afterdepolarization (EAD), which are same as in-vitro model. In-silico model revealed this EAD was mostly related to robust late-mode of sodium current occurred by Na+ overload and suggested that mexiletine is capable of reducing arrhythmia. Afterward, we applicated mexiletine onto hiPSC-CMs mutant model and found mexiletine suppress EADs. Conclusions Precise in-silico disease model can elucidate complicated ion currents and contribute predicting result of drug-testing. Funding Acknowledgement Type of funding source: Public Institution(s). Main funding source(s): Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists

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Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study

Chemosensors ◽

10.3390/chemosensors9030052 ◽

2021 ◽

Vol 9 (3) ◽

pp. 52

Author(s):

Nermine V. Fares ◽

Passant M. Medhat ◽

Christine M. El Maraghy ◽

Sherif Okeil ◽

Miriam F. Ayad

Keyword(s):

Silver Nanoparticle ◽

Carbon Nanodots ◽

Carbon Paste ◽

X Ray Diffraction ◽

Loteprednol Etabonate ◽

Carbon Paste Electrodes ◽

Transmission Electron ◽

Nernstian Slope ◽

Nitrogen Doped Carbon ◽

Modified Carbon Paste Electrodes

Two inexpensive and simple methods for synthesis of carbon nanodots were applied and compared to each other, namely a hydrothermal and microwave-assisted method. The synthesized carbon nanodots were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis), photoluminescence (PL), Fourier transform-infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The synthesized microwave carbon nanodots had smaller particle size and were thus chosen for better electrochemical performance. Therefore, they were used for our modification process. The proposed electrodes performance characteristics were evaluated according to the IUPAC guidelines, showing linear response in the concentration range 10−6–10−2, 10−7–10−2, and 10−8–10−2 M of tobramycin with a Nernstian slope of 52.60, 58.34, and 57.32 mV/decade for the bare, silver nanoparticle and carbon nanodots modified carbon paste electrodes, respectively. This developed potentiometric method was used for quantification of tobramycin in its co-formulated dosage form and spiked human plasma with good recovery percentages and without interference of the co-formulated drug loteprednol etabonate and excipients.

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