A Low-Cost Real-Time Three-Dimensional Confocal Fluorescence Endomicroscopy Imaging System

2011 ◽  
Author(s):  
Feng Zhao ◽  
T.M. McGinnity
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Low Cost ◽  
Three Dimensional ◽  
Confocal Fluorescence

scholarly journals Voxx: a PC-based, near real-time volume rendering system for biological microscopy

2002 ◽  
Vol 282 (1) ◽  
pp. C213-C218 ◽  
Author(s):  
Jeffrey L. Clendenon ◽  
Carrie L. Phillips ◽  
Ruben M. Sandoval ◽  
Shiaofen Fang ◽  
Kenneth W. Dunn
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Low Cost ◽  
Three Dimensional ◽  
Image Data ◽  
Complex Structures ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Biological Image

Confocal and two-photon fluorescence microscopy have advanced the exploration of complex, three-dimensional biological structures at submicron resolution. We have developed a voxel-based three-dimensional (3-D) imaging program (Voxx) capable of near real-time rendering that runs on inexpensive personal computers. This low-cost interactive 3-D imaging system provides a powerful tool for analyzing complex structures in cells and tissues and encourages a more thorough exploration of complex biological image data.

scholarly journals Application of Multiparametric Intraoperative Ultrasound in Glioma Surgery

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Ji Shi ◽  
Ye Zhang ◽  
Bing Yao ◽  
Peixin Sun ◽  
Yuanyuan Hao ◽  
...  
Keyword(s):  
Real Time ◽  
Low Cost ◽  
Three Dimensional ◽  
Intraoperative Ultrasound ◽  
Ultrasound Elastography ◽  
Primary Malignancy ◽  
Glioma Surgery ◽  
Attractive Option ◽  
Ultrasound Technology ◽  
Shape Descriptions

Gliomas are the most invasive and fatal primary malignancy of the central nervous system that have poor prognosis, with maximal safe resection representing the gold standard for surgical treatment. To achieve gross total resection (GTR), neurosurgery relies heavily on generating continuous, real-time, intraoperative glioma descriptions based on image guidance. Given the limitations of currently available equipment, developing a real-time image-guided resection technique that provides reliable functional and anatomical information during intraoperative settings is imperative. Nowadays, the application of intraoperative ultrasound (IOUS) has been shown to improve resection rates and maximize brain function preservation. IOUS, which presents an attractive option due to its low cost, minimal operational flow interruptions, and lack of radiation exposure, is able to provide real-time localization and accurate tumor size and shape descriptions while helping distinguish residual tumors and addressing brain shift. Moreover, the application of new advancements in ultrasound technology, such as contrast-enhanced ultrasound, three-dimensional ultrasound, navigable ultrasound, ultrasound elastography, and functional ultrasound, could help to achieve GTR during glioma surgery. The current review describes current advancements in ultrasound technology and evaluates the role and limitation of IOUS in glioma surgery.

scholarly journals Development of an X-ray real-time stereo imaging technique using synchrotron radiation

2011 ◽  
Vol 18 (4) ◽  
pp. 569-574 ◽  
Author(s):  
Masato Hoshino ◽  
Kentaro Uesugi ◽  
James Pearson ◽  
Takashi Sonobe ◽  
Mikiyasu Shirai ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Real Time ◽  
Imaging System ◽  
Silicon Crystal ◽  
Three Dimensional ◽  
Stereo Image ◽  
Large Field ◽  
Depth Information ◽  
Stereo Imaging ◽  
X Ray

An X-ray stereo imaging system with synchrotron radiation was developed at BL20B2, SPring-8. A portion of a wide X-ray beam was Bragg-reflected by a silicon crystal to produce an X-ray beam which intersects with the direct X-ray beam. Samples were placed at the intersection point of the two beam paths. X-ray stereo images were recorded simultaneously by a detector with a large field of view placed close to the sample. A three-dimensional wire-frame model of a sample was created from the depth information that was obtained from the lateral positions in the stereo image. X-ray stereo angiography of a mouse femoral region was performed as a demonstration of real-time stereo imaging. Three-dimensional arrangements of the femur and blood vessels were obtained.

scholarly journals A Quantitative Method to Measure Low Levels of ROS in Nonphagocytic Cells by Using a Chemiluminescent Imaging System

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Jun-Sub Kim ◽  
Kyuho Jeong ◽  
James M. Murphy ◽  
Yelitza A. R. Rodriguez ◽  
Ssang-Taek Steve Lim
Keyword(s):  
Real Time ◽  
Intracellular Signaling ◽  
Imaging System ◽  
Low Cost ◽  
Fiber Formation ◽  
Imaging Method ◽  
Actin Stress Fiber ◽  
Dependent Manner ◽  
Protein Tyrosine ◽  
Low Levels

Chemiluminescence (CL) is one of the most useful methods for detecting reactive oxygen species (ROS). Although fluorescence dyes or genetically encoded biosensors have been developed, CL is still used due to its high sensitivity, ease of use, and low cost. While initially established and used to measure high levels of ROS in phagocytic cells, CL assays are not ideal for measuring low levels of ROS. Here, we developed a newly modified CL assay using a chemiluminescent imaging system for measuring low concentrations of ROS in nonphagocytic cells. We found that dissolving luminol in NaOH, rather than DMSO, increased the H2O2-induced CL signal and that the addition of 4-iodophenylboronic acid (4IPBA) further increased CL intensity. Our new system also increased the rate and intensity of the CL signal in phorbol 12-myristate 13-acetate- (PMA-) treated HT-29 colon cancer cells compared to those in luminol only. We were able to quantify ROS levels from both cells and media in parallel using an H2O2standard. A significant benefit to our system is that we can easily measure stimulus-induced ROS formation in a real-time manner and also investigate intracellular signaling pathways from a single sample simultaneously. We found that PMA induced tyrosine phosphorylation of protein tyrosine kinases (PTKs), such as focal adhesion kinase (FAK), protein tyrosine kinase 2 (Pyk2), and Src, and increased actin stress fiber formation in a ROS-dependent manner. Interestingly, treatment with either N-acetyl-L-cysteine (NAC) or diphenyleneiodonium (DPI) reduced the PMA-stimulated phosphorylation of these PTKs, implicating a potential role in cellular ROS signaling. Thus, our newly optimized CL assay using 4IPBA and a chemiluminescent imaging method provides a simple, real-time, and low-cost method for the quantification of low levels of ROS.

Low-cost three-dimensional millimeter-wave holographic imaging system based on a frequency-scanning antenna

2017 ◽  
Vol 57 (1) ◽  
pp. A65
Author(s):  
Vahid Amin Nili ◽  
Ehsan Mansouri ◽  
Zahra Kavehvash ◽  
Mohammad Fakharzadeh ◽  
Mahdi Shabany ◽  
...  
Keyword(s):  
Millimeter Wave ◽  
Imaging System ◽  
Low Cost ◽  
Three Dimensional ◽  
Holographic Imaging ◽  
Frequency Scanning ◽  
Scanning Antenna

Design on Light Geodesic Instrument Semi-Physical Simulation Training System Based on Virtual Scene

2014 ◽  
Vol 926-930 ◽  
pp. 1517-1521
Author(s):  
Xiang Jin Wang ◽  
Guo Dong Li ◽  
Zhi Lu Zhang ◽  
Zhe Li
Keyword(s):  
Real Time ◽  
Simulation Training ◽  
Physical Simulation ◽  
Low Cost ◽  
Three Dimensional ◽  
General Purpose ◽  
Training System ◽  
Time Data ◽  
Virtual Scene ◽  
Geodesic Instrument

This paper takes the light geodesic instrument as the research object, puts forward a design idea of the semi-physical simulation training system based on the virtual scene and realizes three-dimensional modeling, real-time scene drawing and real-time data driving display through Virtools and Visual C++. ARM7 and the general-purpose single-chip microcomputer are adopted to realize the function simulation of the equipment. This simulation training system has the characteristics of low cost, low power consumption and high simulation degree.

Wavefront Coded Imaging Systems for MEMS Analysis

2002 ◽  
Author(s):  
Daniel L. Barton ◽  
Jeremy A. Walraven ◽  
Edward R. Dowski ◽  
Rainer Danz ◽  
Andreas Faulstich ◽  
...  
Keyword(s):  
Signal Processing ◽  
Real Time ◽  
High Performance ◽  
Imaging System ◽  
Three Dimensional ◽  
Imaging Systems ◽  
Depth Of Field ◽  
Real Time Imaging ◽  
Large Depth ◽  
Wavefront Coding

Abstract A new imaging technique called Wavefront Coding allows real-time imaging of three-dimensional structures over a very large depth. Wavefront Coding systems combine aspheric optics and signal processing to achieve depth of fields ten or more times greater than that possible with traditional imaging systems. Understanding the relationships between traditional and modern imaging system design through Wavefront Coding is very challenging. In high performance imaging systems nearly all aspects of the system that could reduce image quality are carefully controlled. Modifying the optics and using signal processing can increase the amount of image information that can be recorded by microscopes. For a number of applications this increase in information can allow a single image to be used where a number of images taken at different object planes had been used before. Having very large depth of field and real-time imaging capability means that very deep structures such as surface micromachined MEMS can be clearly imaged with one image, greatly simplifying defect and failure analysis.

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