By analyzing the current situations of the common car transport live fish, the paper designs a monitoring control system of refrigeration and oxygenation for live fish transportation, which uses dissolved oxygen sensors and temperature sensors to achieve real-time monitoring of dissolved oxygen and temperature of the water in the tank during transport, analyze and study the conditions, and take timely measures to eliminate the adverse effects. The system also has an independent diesel engine driving force which separates with the power of the truck, and the system is truly free from the impact of traffic and a variety of emergencies, which maintains the living environment for live fish. Thereby, it enhanced the survival rate of live fish, which thoroughly solved the problems in the long distance transportation for live fish transportation clients and greatly increased the economic interest and social benefits.