The assessment of the loading sequence on fatigue crack growth of cracked details is essential when the ship hull is subjected to random wave-induced loading during the service time. In this paper, the effect of loading sequence on the crack growth life is investigated in order to find a physical engineering method to generate fatigue loading spectrum for ship fatigue assessment. The long term distribution of fatigue loading and the unique crack growth rate curve model have been employed in the analysis. The results show that the shape parameter affects the fatigue life significantly if the long-term distribution of wave-induced stress range is modeled by two-parameter Weibull distribution. Moreover, the spectral-based method provides a reasonable fatigue loading spectrum and avoid the confusion in determining the shape parameter for different empirical formulas, which are recommended by several main ship classification societies. An example of fatigue assessment for a cracked detail in a container ship is demonstrated as a reference for fatigue assessment of a ship hull based on crack growth.