Observation of residual surface charge distribution inside an artificial air-filled void due to partial discharge activities at room and liquid nitrogen temperatures

Author(s):  
T. Kurihara ◽  
J. Suehiro ◽  
M. Hara
Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.


2010 ◽  
Vol 12 (12) ◽  
pp. 1718-1721 ◽  
Author(s):  
Sergey Sigalov ◽  
Mikhael D. Levi ◽  
Gregory Salitra ◽  
Doron Aurbach ◽  
Joachim Maier

Nanoscale ◽  
2021 ◽  
Author(s):  
Feng Chen ◽  
Jin He ◽  
Prakash Manandhar ◽  
Yizi Yang ◽  
Peidang Liu ◽  
...  

The distribution of surface charge and potential of cell membrane plays an indispensable role in cellular activities. However, probing surface charge of live cells in physiological conditions, until recently, remains...


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