Study of In Vitro Diagnostic Preconcentration Technique via Surface Acoustic Wave Modified with the 54 MHz Communication Chip

A microfluidic biosensor with surface acoustic wave technology was used in this study to monitor the interaction of calcium carbonate with standard carboxylate self-assembled monolayer sensor chips. Different fluids, with and without biomolecular components, were investigated. The pH-dependent surface interactions of two bio-inspired cationic peptides, AS8 and ES9, which are similar to an extracellular domain of the chitin synthase involved in mollusc shell formation, were also investigated in a biological buffer system. A range of experimental conditions are described that are suitable to study non-covalent molecular interactions in the presence of ionic substances, such as, mineral precursors below the solubility equilibrium. The peptide ES9, equal to the mollusc chitin synthase epitope, is less sensitive to changes in pH than its counterpart AS8 with a penta-lysine core, which lacks the flanking acidic residues. This study demonstrates the extraordinary potential of microfluidic surface acoustic wave biosensors to significantly expand our experimental capabilities for studying the principles underlying biomineralization in vitro.

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In vitro Evaluation of a Surface Acoustic Wave Device on Epidermal Characteristics

Journal of Clinical & Experimental Dermatology Research ◽

10.4172/2155-9554.1000407 ◽

2017 ◽

Vol 08 (04) ◽

Author(s):

Jonathan Rosenblum

Keyword(s):

Acoustic Wave ◽

Surface Acoustic Wave ◽

In Vitro Evaluation ◽

Surface Acoustic Wave Device ◽

Acoustic Wave Device ◽

Wave Device

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A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)

Diagnostics ◽

10.3390/diagnostics11101838 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1838

Author(s):

Yu-Chi Peng ◽

Chia-Hsuan Cheng ◽

Hiromi Yatsuda ◽

Szu-Heng Liu ◽

Shih-Jen Liu ◽

...

Keyword(s):

Acoustic Wave ◽

Surface Acoustic Wave ◽

Pathogen Detection ◽

Rapid Test ◽

Horizontal Surface ◽

Enzyme Linked Immunosorbent Assay ◽

Antibody Testing ◽

N Protein ◽

Shear Horizontal

Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.

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