Comparison of CT contrast blood pool agents for in-vivo 3D angiography using MicroCT

SummaryThe technique of blood pool scanning was used to examine 15 hemophilic subjects. Employing an in vivo method for erythrocyte labeling with Technetium-99 m, a dynamic perfusion sequence is obtained using a scintillation camera positioned over the area to be examined. This demonstrates the vascularity of the tissue. Subsequently, equilibrium blood pool images of the area are obtained and analyzed with a densitometer to assess relative regional blood volume. In patients who were not bleeding but had chronic arthropathy, vascularity was not increased, and the blood volume of comparable joints was similar. By contrast, marked increases in vascularity and image density were observed in studies of acutely bleeding joints. Chronic hemarthroses were associated with persistent, but less marked increases in joint perfusion. Transient increases in joint vascularity were demonstrated after insertion of knee prostheses. In a patient with a thigh hematoma, the dimensions of the hemorrhage were clearly delineated. Since only a tracer dose of nuclide is infused intravenously, there are no allergic reactions or other side effects of the procedure. Blood pool scanning is a safe, non-invasive technique that augments clinical and radiographic evaluations, and provides a new dimension in the assessment of the hemophilic patient.

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Blood Pool Agents for Magnetic Resonance Angiography

Journal of Vascular and Interventional Radiology ◽

10.1016/s1051-0443(98)70135-6 ◽

1998 ◽

Vol 9 (1) ◽

pp. 246

Author(s):

E. Kent Yucel

Keyword(s):

Magnetic Resonance ◽

Magnetic Resonance Angiography ◽

Blood Pool ◽

Blood Pool Agents

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La gadodiamide come mezzo di contrasto in tomografia computerizzata cranio-encefalica

Rivista di Neuroradiologia ◽

10.1177/197140090201500606 ◽

2002 ◽

Vol 15 (6) ◽

pp. 705-711

Author(s):

P. Renzetti ◽

R. C. Parodi ◽

C. Ottonello ◽

F. Zandrino ◽

M. Cossu ◽

...

Keyword(s):

Sono State ◽

Risonanza Magnetica ◽

Atomic Weight ◽

Tomografia Computerizzata ◽

Mezzi Di Contrasto ◽

Sono Stati ◽

Nello Studio ◽

Ct Contrast

L'elevato peso atomico del Gd giustifica l'ipotesi di un utilizzo in tomografia computerizzata (TC) di mezzi di contrasto (MdC) già clinicamente in uso in risonanza magnetica (RM). Il potenziamento TC determinato dalla Gadodiamide (Gd-DTPA-BMA, Omniscan, Nycomed-Amersham), MdC paramagnetico non ionico, è stato valutato e quantificato in vitro e in vivo. Due serie di soluzioni scalari di Gadodiamide e di MdC iodato (Iopamiro 370, Bracco) sono state sottoposte a scansione TC per la quantificazione densitometrica in unità Hounsfield (UH). Sette pazienti affetti da neoplasia intracranica sono stati sottoposti a TC prima e dopo somministrazione endovenosa di 0,3 mmol/Kg di Gadodiamide; sono stati rilevati i valori medi di densità pre- e postcontrasto a livello dell'arteria basilare e della massa tumorale. Nello studio in vitro, a parità di concentrazione molare del MdC, è risultata maggiore la densità media della soluzione di gadodiamide rispetto al MdC iodato, superiorità statisticamente significativa (test F, p < 0,0001), a conferma del fatto che il Gd ha caratteristiche fisiche che lo rendono utilizzabile in MdC per TC. Nello studio in vivo, la gadodiamide ha determinato incrementi densitometrici medi (postcontrasto /precontrasto) del 71,05% per l'arteria basilare e del 45,23% per la lesione tumorale, consentendo una sufficiente apprezzabilità soggettiva dell'enhancement. La Gadodiamide può essere utilizzata come MdC in TC in pazienti con dubbia o asserita diatesi allergica per i MdC iodati allorquando non sia praticamente disponibile la RM (urgenze!) o sussistano importanti controindicazioni (pacemaker, ecc.). L'osmolarità medio-bassa (780 mOsm/Kg) e il profilo tossi-cologico favorevole della Gadodiamide permettono di ipotizzare l'utilizzo di dosi anche più elevate. Tali risultati preliminari rafforzano l'ipotesi della messa a punto di MdC per TC a base di Gd; più atomi di Gd potrebbero ad esempio essere contenuti all'interno della molecola con il duplice effetto di ridurre la tossicità ed elevare il peso atomico del MdC. Gadolinium (Gd) high atomic weight can enable us to use the Gd-chelates as contrast agents (c.a.) in computed tomography (CT). CT contrast enhancement (c.e.) due to Gadodiamide (Gd-DTPA-BMA, Omniscan, Nycomed-Amersham), a non-ionic paramagnetic c.a. used in magnetic resonance (MR) imaging, was evaluated and quantified through an in vitro and in vivo study.

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Improved mapping of pharmacologically induced neuronal activation using superparamagnetic iron blood pool agents

NeuroImage ◽

10.1016/s1053-8119(00)91501-5 ◽

2000 ◽

Vol 11 (5) ◽

pp. S570

Author(s):

Y.I. Chen ◽

J.B. Mandeville ◽

T.V. Nguyen ◽

F. Cavagna ◽

B.G. Jenkins

Keyword(s):

Blood Pool ◽

Neuronal Activation ◽

Blood Pool Agents

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Blood Pool Agents for MRA

Seminars in Interventional Radiology ◽

10.1055/s-2008-1057048 ◽

1998 ◽

Vol 15 (02) ◽

pp. 215-222 ◽

Cited By ~ 2

Author(s):

E. Yucel ◽

Randall Lauffer

Keyword(s):

Blood Pool ◽

Blood Pool Agents

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In Vivo Quantification of Myocardial Infarction in Mice Using Micro-CT and a Novel Blood Pool Agent

Contrast Media & Molecular Imaging ◽

10.1155/2017/2617047 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Stefan Sawall ◽

Danielle Franke ◽

Anne Kirchherr ◽

Jan Beckendorf ◽

Jan Kuntz ◽

...

Keyword(s):

Myocardial Infarction ◽

Contrast Agent ◽

Infarct Size ◽

Blood Pool ◽

Signal Enhancement ◽

Imaging Method ◽

Micro Ct ◽

Contrast Agent Injection ◽

Dependent Signal

We herein developed a micro-CT method using the innovative contrast agent ExiTron™ MyoC 8000 to longitudinally monitor cardiac processes in vivo in small animals. Experiments were performed on healthy mice and mice with myocardial infarction inflicted by ligation of the left anterior descending artery. Time-dependent signal enhancement in different tissues of healthy mice was measured and various contrast agent doses were investigated so as to determine the minimum required dose for imaging of the myocardium. Due to its ability to be taken up by healthy myocardium but not by infarct tissue, ExiTron MyoC 8000 enables detection of myocardial infarction even at a very low dose. The signal enhancement in the myocardium of infarcted mice after contrast agent injection was exploited for quantification of infarct size. The values of infarct size obtained from the imaging method were compared with those obtained from histology and showed a significant correlation (R2=0.98). Thus, the developed micro-CT method allows for monitoring of a variety of processes such as cardiac remodeling in longitudinal studies.

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Abstract 3206: Quantitative Rage Imaging In Diabetic And Non-diabetic ApoE Null Mice

Circulation ◽

10.1161/circ.118.suppl_18.s_1112-c ◽

2008 ◽

Vol 118 (suppl_18) ◽

Author(s):

Yared Tekabe ◽

Joane Luma ◽

Marija Sedler ◽

Rosa Rosario ◽

Ann Marie Schmidt ◽

...

Keyword(s):

New York ◽

Rhode Island ◽

Heart Association ◽

Lesion Size ◽

Blood Pool ◽

Spect Imaging ◽

Mean Values ◽

Pilot Sample ◽

Rage Expression

Receptors for Advanced Glycated Endproducts (RAGE) bind AGEs and other inflammatory ligands and are expressed in atherosclerotic plaques in diabetic and non-diabetic subjects. The higher expression in diabetes (DM) corresponds with accelerated course of the disease. This study was designed to test the hypothesis that the level of RAGE expression in atherosclerosis can be detected in-vivo by quantitative in-vivo SPECT imaging. Methods and Results : A monoclonal murine antibody was developed against the V-domain of RAGE, fragmented into F(ab′) 2 and labeled with 99m Tc and dose of 402 ± 18 μCi or 14.8 ± 0.67 MBq injected into eleven 16 –24 week apoE −/− mice 5 with streptozotocin induced DM (given at 6 weeks). Four hours later (time based on blood pool clearance) mice were imaged on HiSPECT scanner (Bioscan), sacrificed, the proximal aorta removed, counted, and sectioned. Uptake in the thorax on SPECT corresponding to the proximal aorta was quantified as % ID using Interview XP (Mediso) software. For the total vascular area for each section, lesion size as % area and cells staining positive for RAGE as % total cells were quantified using immunohistomorphometry. Results expressed as mean values for the apoE −/− with and without DM are shown below. Lesion morphology was AHA class II-III and size ranged from 4 –56%. Values for % ID/g tissue and for % ID from scans for all mice were plotted against RAGE expression (% RAGE + cells) and the correlations were the following: % ID/g tissue vs. % RAGE + cells, R 2 = 0.80, P = 0.0013, and for % ID scan vs. % RAGE + cells, R 2 = 0.83, P = 0.0002. Conclusions : In this pilot sample 99m Tc-labeled anti-RAGE F(ab′) 2 SPECT imaging successfully identified early accelerated atherosclerosis in diabetic apoE −/− mice compared to age matched non-diabetic apoE −/− mice and quantified RAGE expression over a range of lesion severities. This research has received full or partial funding support from the American Heart Association, AHA Founders Affiliate (Connecticut, Maine, Massachusetts, New Hampshire, New Jersey, New York, Rhode Island, Vermont).

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