Action potential sources and their volume conductor fields

1977 ◽  
Vol 65 (5) ◽  
pp. 601-611 ◽  
Author(s):  
R. Plonsey
Keyword(s):  
Action Potential ◽  
Volume Conductor ◽  
Potential Sources

scholarly journals An oscillatory component of propagated fluctuation electric potential in lupine shoot

2014 ◽  
Vol 55 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Zygmunt Hejnowicz ◽  
Andrzej Pijanowski ◽  
Krzysztof Głębicki
Keyword(s):  
Action Potential ◽  
Electric Potential ◽  
Volume Conductor ◽  
Excitable Cells ◽  
Oscillatory Component ◽  
Band Pass ◽  
Cut Surface

Application of a drop of auxin solution to a cut surface on the petiole in lupine shoot elicits a travelling pulse of electric potential decrease. This pulse was simultaneously recorded by means of a DC amplifier and band-pass amplifier 0.1-100 Hz, both connected to the same exploring AgCl electrode driven into the stem. The DC record shows a pulse 20-80 mV in height of about 30 s duration at its height with smooth slopes. The band-pass amplifier shows one to a few pairs of spikes (negative and positive) whose amplitude is at least of an order lower than that of the DC pulse. These spikes are interpreted as the action potential of certain excitable cells recorded in a "volume conductor". The pulse is interpreted as a wave of cooperative depolarization of excitable and a mass of inexcitable cells.

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

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