Plasmonic nanoparticles based flexible micro stripe pattern for cellular behavior regulation and localized pH detection

AbstractMechanical stimulation of single cells has been shown to affect cellular behavior from the molecular scale to ultimate cell fate including apoptosis and proliferation. In this, the ability to control the spatiotemporal application of force on cells through their extracellular matrix connections is critical to understand the cellular response of mechanotransduction. Here, we develop and utilize a novel pressure-driven equibiaxial cell stretching device (PECS) combined with an elastomeric material to control specifically the mechanical stimulation on single cells. Cells were cultured on silicone membranes coated with molecular matrices and then a uniform pressure was introduced to the opposite surface of the membrane to stretch single cells equibiaxially. This allowed us to apply mechanical deformation to investigate the complex nature of cell shape and structure. These results will enhance our knowledge of cellular and molecular function as well as provide insights into fields including biomechanics, tissue engineering, and drug discovery.

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A Photoactivatable α5β1-Specific Integrin Ligand

10.26434/chemrxiv.6097688 ◽

2018 ◽

Author(s):

Roshna Vakkeel ◽

Aleeza Farrukh ◽

Aranzazu del Campo

Keyword(s):

Cellular Response ◽

Light Exposure ◽

Matrix Composition ◽

Cellular Behavior ◽

Dynamic Variations ◽

In Situ Activation ◽

Controlled Exposure ◽

Cellular Behaviour ◽

Integrin Ligand

In order to study how dynamic changes of α5β1 integrin engagement affect cellular behaviour, photoactivatable derivatives of α5β1 specific ligands are presented in this article. The presence of the photoremovable protecting group (PRPG) introduced at a relevant position for integrin recognition, temporally inhibits ligand bioactivity. Light exposure at cell-compatible dose efficiently cleaves the PRPG and restores functionality. Selective cell response (attachment, spreading, migration) to the activated ligand on the surface is achieved upon controlled exposure. Spatial and temporal control of the cellular response is demonstrated, including the possibility to in situ activation. Photoactivatable integrin-selective ligands in model microenvironments will allow the study of cellular behavior in response to changes in the activation of individual integrins as consequence of dynamic variations of matrix composition.

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Faculty Opinions recommendation of A C. elegans model of nicotine-dependent behavior: regulation by TRP-family channels.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1048236.500152 ◽

2006 ◽

Author(s):

Marina Picciotto

Keyword(s):

Behavior Regulation ◽

C Elegans ◽

Dependent Behavior

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Influence of MWNTs/PMMA Thin Film on Cellular Behavior of Cardiac Stem Cells From Brown Adipose

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1206.2013.00289 ◽

2013 ◽

Vol 40 (10) ◽

pp. 1070

Author(s):

Cui-Mi DUAN ◽

Hong-Yu SUN ◽

Ye YUAN ◽

Zhi-Qiang LIU ◽

Rong-Yu TANG ◽

...

Keyword(s):

Thin Film ◽

Stem Cells ◽

Cardiac Stem Cells ◽

Cellular Behavior ◽

Brown Adipose

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Observing electrochemistry on single plasmonic nanoparticles

Electrochemical Science Advances ◽

10.1002/elsa.202100115 ◽

2021 ◽

Author(s):

Chao Jing ◽

Yi‐Tao Long

Keyword(s):

Plasmonic Nanoparticles

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Toxicological Profile of Plasmonic Nanoparticles in Zebrafish Model

International Journal of Molecular Sciences ◽

10.3390/ijms22126372 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6372

Author(s):

Marta d’Amora ◽

Vittoria Raffa ◽

Francesco De Angelis ◽

Francesco Tantussi

Keyword(s):

Surface Plasmon Resonance ◽

Adverse Effects ◽

Physicochemical Properties ◽

Surface Plasmon ◽

Danio Rerio ◽

Plasmonic Nanoparticles ◽

Comprehensive Understanding ◽

Zebrafish Model ◽

Toxicological Profile ◽

Materials Used

Plasmonic nanoparticles are increasingly employed in several fields, thanks to their unique, promising properties. In particular, these particles exhibit a surface plasmon resonance combined with outstanding absorption and scattering properties. They are also easy to synthesize and functionalize, making them ideal for nanotechnology applications. However, the physicochemical properties of these nanoparticles can make them potentially toxic, even if their bulk metallic forms are almost inert. In this review, we aim to provide a more comprehensive understanding of the potential adverse effects of plasmonic nanoparticles in zebrafish (Danio rerio) during both development and adulthood, focusing our attention on the most common materials used, i.e., gold and silver.

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