scholarly journals Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor β subunit

2009 ◽  
Vol 13 (10) ◽  
pp. 2814-2818 ◽  
Author(s):  
Thomas U. Schwartz ◽  
Rudolf Walczak ◽  
Günter Blobel
Keyword(s):  
Signal Recognition Particle ◽  
Circular Permutation ◽  
Signal Recognition ◽  
Β Subunit ◽  
Surface Entropy ◽  
Signal Recognition Particle Receptor

scholarly journals Pirh2 interacts with and ubiquitylates signal recognition particle receptor β subunit

2008 ◽  
Vol 29 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Kenji ABE ◽  
Takayuki HATTORI ◽  
Tomoyasu ISOBE ◽  
Kyoko KITAGAWA ◽  
Toshiaki ODA ◽  
...  
Keyword(s):  
Signal Recognition Particle ◽  
Signal Recognition ◽  
Β Subunit ◽  
Signal Recognition Particle Receptor

scholarly journals The Ribosome Regulates the Gtpase of the β-Subunit of the Signal Recognition Particle Receptor

1999 ◽  
Vol 146 (4) ◽  
pp. 723-730 ◽  
Author(s):  
Gerald Bacher ◽  
Martin Pool ◽  
Bernhard Dobberstein
Keyword(s):  
Protein Targeting ◽  
Signal Sequence ◽  
Signal Recognition Particle ◽  
Bound State ◽  
Signal Recognition ◽  
Β Subunit ◽  
Gtp Hydrolysis ◽  
Signal Recognition Particle Receptor ◽  
Membrane Bound ◽  
Gtpase Cycle

Protein targeting to the membrane of the ER is regulated by three GTPases, the 54-kD subunit of the signal recognition particle (SRP) and the α- and β-subunit of the SRP receptor (SR). Here, we report on the GTPase cycle of the β-subunits of the SR (SRβ). We found that SRβ binds GTP with high affinity and interacts with ribosomes in the GTP-bound state. Subsequently, the ribosome increases the GTPase activity of SRβ and thus functions as a GTPase activating protein for SRβ. Furthermore, the interaction between SRβ and the ribosome leads to a reduction in the affinity of SRβ for guanine nucleotides. We propose that SRβ regulates the interaction of SR with the ribosome and thereby allows SRα to scan membrane-bound ribosomes for the presence of SRP. Interaction between SRP and SRα then leads to release of the signal sequence from SRP and insertion into the translocon. GTP hydrolysis then results in dissociation of SR from the ribosome, and SRP from the SR.

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