Pain modulatory network is influenced by sex and age in a healthy state and during osteoarthritis progression in rats

Osteoarthritis is a most widespread chronic degenerative joint disease that causes pain, cartilage deformation, and joint inflammation. Adverse alterations of intestinal microbiota like dysbiosis may lead to metabolic syndrome and inflammation, two important components of osteoarthritis progression. Aim. In this study we investigated the effect of chondroitin sulfate and probiotics on the gut microbiome in monoiodoacetate-induced osteoarthritis model in rats. Methods. The species and quantitative composition of feces were determined using diagnostic media with selective properties. Further identification of isolated microorganisms was carried out according to morphological, tinctorial, physiological and metabolic parameters. The results are presented in the form of lg CFU/g. Results. Induction of osteoarthritis caused significant increasing the number of opportunistic enterobacteria and lactose-negative Escherichia coli against the decreasing of lacto- and bifidobacteria that may indicate a dysbiotic condition. Coadministration of chondroitin sulfate and probiotic bacteria has led to improvement the quantitative composition of the gut microbiota in experimental animals, the numerous of Bifidobacterium, Lactobacillus were increasing against decreasing the quantitative composition of opportunistic microorganisms. Conclusions. Monoiodoacetate-induced osteoarthritis caused dysbiosis of gut in rat. We observed beneficial effect of combined administration of chondroitin sulfate and probiotics on gut microbiota composition in rats with experimental osteoarthritis. Thus, adding of supplements like probiotics to standard treatment of osteoarthritis may have potentials to prevent and treat this disease.

Download Full-text

Ayurveda dietary guidelines during Covid-19 Pandemic

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl1.3697 ◽

2020 ◽

Vol 11 (SPL1) ◽

pp. 1477-1481

Author(s):

Ishwari Gaikwad ◽

Priyanka Shelotkar

Keyword(s):

Dietary Habits ◽

Relevant Literature ◽

Dietary Guidelines ◽

The Body ◽

Daily Regimen ◽

Web Page ◽

Healthy State ◽

Health Disorders ◽

The Individual ◽

The Right

The current world situation is both frightening and alarming due to the massive disruption caused by the Covid-19 pandemic. The next few days are censorious as we need to be very precautious in our daily regimen as well as dietary habits. Ayurveda offers knowledge about food based on certain reasoning. Indecent food custom is the chief cause for the rising development of health disorders in the current era. In classical texts of Ayurveda, the concept of diet explained well, ranging from their natural sources, properties and specific utility in pathological as well as physiological manner. In this work, the review of the relevant literature of Ahara (Diet) was carried out from Charak Samhita and other texts, newspapers, articles, web page related to the same. Every human being is unique with respect to his Prakriti (Physical and mental temperament), Agni (Digestive capacity), Koshtha (Nature of bowel) etc. For that reason, the specificity of the individual should be kept in mind. Ahara, when consumed in the appropriate amount at the right moment following all Niyamas (Guidelines) given in Ayurveda texts, gives immunity and keeps the body in a healthy state during pandemics such as Covid-19. Ultimately, this will help the human body to maintain its strength for life. This article reviews the concept of diet viz. combination of foods, their quantity and quality, methods of preparation and processing, which are to be followed during pandemics and are essential in maintenance and endorsement of health and preclusion of diseases.

Download Full-text

Faculty Opinions recommendation of Intra-articular delivery of adipose derived stromal cells attenuates osteoarthritis progression in an experimental rabbit model.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717997763.793474298 ◽

2013 ◽

Author(s):

Mats Brittberg

Keyword(s):

Stromal Cells ◽

Rabbit Model ◽

Adipose Derived Stromal Cells ◽

Osteoarthritis Progression

Download Full-text

Decreased miR-214–3p activates NF-κB pathway and aggravates osteoarthritis progression

EBioMedicine ◽

10.1016/j.ebiom.2021.103283 ◽

2021 ◽

Vol 65 ◽

pp. 103283

Author(s):

Yumei Cao ◽

Su'an Tang ◽

Xiaoyu Nie ◽

Zuoqing Zhou ◽

Guangfeng Ruan ◽

...

Keyword(s):

Osteoarthritis Progression

Download Full-text

The dysbiosis of ovine foot microbiome during the development and treatment of contagious ovine digital dermatitis

Animal Microbiome ◽

10.1186/s42523-021-00078-4 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

J. S. Duncan ◽

J. W. Angell ◽

P. Richards ◽

L. Lenzi ◽

G. J. Staton ◽

...

Keyword(s):

Antibiotic Treatment ◽

Amplicon Sequencing ◽

Strong Tendency ◽

Rrna Gene ◽

Digital Dermatitis ◽

Healthy State ◽

Foot Disease ◽

End Stage ◽

And Control ◽

Interdigital Dermatitis

Abstract Background Contagious Ovine Digital Dermatitis (CODD) is an emerging and common infectious foot disease of sheep which causes severe welfare and economic problems for the sheep industry. The aetiology of the disease is not fully understood and control of the disease is problematic. The aim of this study was to investigate the polybacterial aetiopathogenesis of CODD and the effects of antibiotic treatment, in a longitudinal study of an experimentally induced disease outbreak using a 16S rRNA gene amplicon sequencing approach. Results CODD was induced in 15/30 experimental sheep. During the development of CODD three distinct phenotypic lesion stages were observed. These were an initial interdigital dermatitis (ID) lesion, followed by a footrot (FR) lesion, then finally a CODD lesion. Distinct microbiota were observed for each lesion in terms of microbial diversity, clustering and composition. Porphyromonadaceae, Family XI, Veillonellaceae and Fusobacteriaceae were significantly associated with the diseased feet. Veillonellaceae and Fusobacteriaceae were most associated with the earlier stages of ID and footrot rather than CODD. Following antibiotic treatment of the sheep, the foot microbiota showed a strong tendency to return to the composition of the healthy state. The microbiota composition of CODD lesions collected by swab and biopsy methods were different. In particular, the Spirochaetaceae family were more abundant in samples collected by the biopsy method, suggesting that these bacteria are present in deeper tissues of the diseased foot. Conclusion In this study, CODD presented as part of a spectrum of poly-bacterial foot disease strongly associated with bacterial families Porphyromonadaceae, Family XI (a family in Clostridiales also known as Clostridium cluster XI), Veillonellaceae and Fusobacteriaceae which are predominately Gram-negative anaerobes. Following antibiotic treatment, the microbiome showed a strong tendency to return to the composition of the healthy state. The composition of the healthy foot microbiome does not influence susceptibility to CODD. Based on the data presented here and that CODD appears to be the severest end stage of sheep infectious foot disease lesions, better control of the initial ID and FR lesions would enable better control of CODD and enable better animal welfare.

Download Full-text

Phospholipase A2 inhibitor–loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression

Science Advances ◽

10.1126/sciadv.abe6374 ◽

2021 ◽

Vol 7 (15) ◽

pp. eabe6374

Author(s):

Yulong Wei ◽

Lesan Yan ◽

Lijun Luo ◽

Tao Gui ◽

Biang Jang ◽

...

Keyword(s):

Clinical Success ◽

Joint Space ◽

Therapeutic Agents ◽

Knee Joints ◽

Phospholipid Membrane ◽

Drug Discovery And Development ◽

Knee Oa ◽

Clinical Challenge ◽

Osteoarthritis Progression ◽

Human And Mouse

Treating osteoarthritis (OA) remains a major clinical challenge. Despite recent advances in drug discovery and development, no disease-modifying drug for knee OA has emerged with any notable clinical success, in part, due to the lack of valid and responsive therapeutic targets and poor drug delivery within knee joints. In this work, we show that the amount of secretory phospholipase A2 (sPLA2) enzyme increases in the articular cartilage in human and mouse OA cartilage tissues. We hypothesize that the inhibition of sPLA2 activity may be an effective treatment strategy for OA. To develop an sPLA2-responsive and nanoparticle (NP)–based interventional platform for OA management, we incorporated an sPLA2 inhibitor (sPLA2i) into the phospholipid membrane of micelles. The engineered sPLA2i-loaded micellar NPs (sPLA2i-NPs) were able to penetrate deep into the cartilage matrix, prolong retention in the joint space, and mitigate OA progression. These findings suggest that sPLA2i-NPs can be promising therapeutic agents for OA treatment.

Download Full-text