The potential ecological effects of casein phosphopeptide-amorphous calcium phosphate in dental caries prevention

Background: Dental caries is a multifactorial disease indicated by the progressive demineralisation process of dental tissue. It is caused by an imbalance between the remineralisation and demineralisation processes. The focus of caries management is on prevention. Providing materials that can induce remineralisation is one management of caries prevention. Various materials have been or are being researched, such as casein phosphopeptide amorphous calcium phosphate (CPP–ACP), tricalcium phosphate (fTCP), bioactive glass (BAG), and nanotechnologies such as nano-hydroxyapatite (n-HAP) and silver nano fluorine (NSF). Purpose: This study aims to review the development of enamel remineralisation inducing materials as a newer approach in caries prevention. Review: Various ingredients have been shown to increase enamel remineralisation through different mechanisms in preventing the development of carious lesions. Conclusion: CPP–ACP, fTCP, BAG, n-HAP, and NSF can induce enamel remineralisation as caries prevention agents. n-HAP and NSF are the most effective agents to enhance enamel remineralisation to prevent caries.

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Ecological approaches to dental caries prevention: Alteration of biofilms by natural products and casein-phosphopeptide-amorphous calcium phosphate

10.14264/uql.2019.640 ◽

2019 ◽

Author(s):

Nebu Ivan Philip

Keyword(s):

Natural Products ◽

Calcium Phosphate ◽

Dental Caries ◽

Amorphous Calcium Phosphate ◽

Caries Prevention ◽

Casein Phosphopeptide

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Casein Phosphopeptide-Amorphous Calcium Phosphate Attenuates Virulence and Modulates Microbial Ecology of Saliva-Derived Polymicrobial Biofilms

Caries Research ◽

10.1159/000499869 ◽

2019 ◽

Vol 53 (6) ◽

pp. 643-649 ◽

Cited By ~ 2

Author(s):

Nebu Philip ◽

Shaneen J. Leishman ◽

H.M.H.N. Bandara ◽

Laurence J. Walsh

Keyword(s):

Calcium Phosphate ◽

Relative Abundance ◽

Amorphous Calcium Phosphate ◽

Bacterial Species ◽

Fold Change ◽

Ecological Effects ◽

Oral Microbiome ◽

Ecological Change ◽

Casein Phosphopeptide

Background: Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) acts as a salivary biomimetic that provides bioavailable calcium and phosphate ions to augment fluoride-mediated remineralisation of early caries lesions. However, there are indications that it may also have beneficial ecological effects on the oral microbiome. Objective: This in vitro study investigated whether CPP-ACP could influence microbial counts, acidogenicity, and the relative abundance of specific caries- and health-associated bacterial species in polymicrobial biofilms. Methods: Saliva-derived polymicrobial biofilms were grown for 96 h in a cariogenic environment and treated every 12 h with 2% CPP-ACP or vehicle control. Colony forming units (CFUs) and acidogenicity were estimated from the treated biofilms. Microbial ecological effects of CPP-ACP were assessed based on the relative abundance of 14 specific caries- and health-associated bacterial species using a real-time quantitative PCR assay. Results: CPP-ACP-treated biofilms showed relatively modest, but significant, reductions in microbial CFUs (21% reduction, p = 0.008) and acidogenicity (33% reduction, p < 0.001), compared to the control-treated biofilms. The CPP-ACP treated biofilms also exhibited significantly lower bacterial loads of cariogenic Scardovia wiggsiae (fold change 0.017, p < 0.001) and Prevotella denticola(fold change 0.005, p < 0.001), and higher bacterial loads of commensal Streptococcus sanguinis(fold change 30.22, p < 0.001), S. mitis/oralis(fold change 9.66, p = 0.012), and S. salivarius/thermophilus(fold change 89.35, p < 0.001) than the control-treated biofilms. Conclusions: The results indicate that CPP-ACP has virulence-attenuating attributes that can influence a beneficial microbial ecological change in the biofilm.

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Prevention of dental caries with remineralization in containing Casein Phosphopeptid-Amorphous Calcium Phosphate (CPP-ACP) to students SD Swasta Muhammadiyah – 03 Medan

ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat ◽

10.32734/abdimastalenta.v4i2.4112 ◽

2019 ◽

Vol 4 (2) ◽

pp. 439-443

Author(s):

Fitri Yunita Batubara ◽

M. Zulkarnain ◽

Cut Nurliza ◽

Nurdiana ◽

Dewi Kartika

Keyword(s):

Calcium Phosphate ◽

Dental Caries ◽

Primary School ◽

Community Service ◽

Amorphous Calcium Phosphate ◽

Caries Prevention ◽

School Age ◽

High Caries Risk ◽

Grade Ii ◽

The Impact

Dental caries is an infectious disease caused by demineralization of enamel and dentin which is closely related to the consumption of cariogenic foods. Generally children entering school age have a high caries risk, because at this school age children usually like food and drink snacks as they wish. The impact that occurs when since the beginning has experienced caries is in addition to the function of the teeth as chewing that is disrupted, swollen gums, the child will also experience disturbances in carrying out their daily activities, so the child will not eat and consequently malnutrition can occur, this causes the child to be unable to study because of lack of concentration so that it will affect intelligence. The results of the field analysis and preliminary interviews with school leaders as partners in community service obtained data that students in Muhammadiyah Private Elementary School 03 Medan aged 6-8 years experienced dental caries with the characteristics of cavities, porous, and stains / plaques. These characteristics are related to the patterns or behavior of students who like to consume sweet foods like candy, bread, and chocolate. School Dental Health Efforts (UKGS) have also not been implemented in schools. Therefore, community service is needed to be carried out with a form of socialization prevention for early caries in order to provide knowledge of the main causes, ways of spreading, impact, efforts to prevent dental caries, and providing remineralization (dental caries prevention) containing Casein Phosphopeptid-Amorphous Calcium Phosphate (CPP-ACP) to students of grade II Muhammadiyah Primary School 03 Medan. Community service activities were carried out by five service teams from the Faculty of Dentistry, University of North Sumatra on August 27, 2019, involving as many as twenty two USU FKG students who were undergoing clinical clerkship. The socialization and provision of caries prevention materials to 111 students who were in grade II of Muhammadiyah Primary School - 03 Medan.

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A review of novel dental caries preventive material: Casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) complex

King Saud University Journal of Dental Sciences ◽

10.1016/j.ksujds.2013.03.004 ◽

2013 ◽

Vol 4 (2) ◽

pp. 47-51 ◽

Cited By ~ 7

Author(s):

Imran Farooq ◽

Imran A. Moheet ◽

Zonera Imran ◽

Umer Farooq

Keyword(s):

Calcium Phosphate ◽

Dental Caries ◽

Amorphous Calcium Phosphate ◽

Casein Phosphopeptide

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Randomized Controlled Study to Evaluate Microbial Ecological Effects of CPP-ACP and Cranberry on Dental Plaque

JDR Clinical & Translational Research ◽

10.1177/2380084419859871 ◽

2019 ◽

Vol 5 (2) ◽

pp. 118-126

Author(s):

N. Philip ◽

S.J. Leishman ◽

H.M.H.N. Bandara ◽

D.L. Healey ◽

L.J. Walsh

Keyword(s):

Randomized Controlled Trial ◽

Calcium Phosphate ◽

Dental Caries ◽

Amorphous Calcium Phosphate ◽

Dental Plaque ◽

Controlled Trial ◽

Bacterial Species ◽

Oral Microbiome ◽

Randomized Controlled ◽

Casein Phosphopeptide

Introduction: Ecological approaches to dental caries prevention play a key role in attaining long-term control over the disease and maintaining a symbiotic oral microbiome. Objectives: This study aimed to investigate the microbial ecological effects of 2 interventional dentifrices: a casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) dentifrice and the same dentifrice supplemented with a polyphenol-rich cranberry extract. Methods: The interventional toothpastes were compared with each other and with an active control fluoride dentifrice in a double-blinded randomized controlled trial. Real-time quantitative polymerase chain reaction (qPCR) analysis was used to determine changes in the bacterial loads of 14 key bacterial species (8 caries associated and 6 health associated) in the dental plaque of trial participants after they used the dentifrices for 5 to 6 wk. Results: From the baseline to the recall visit, significant differences were observed between the treatment groups in the bacterial loads of 2 caries-associated bacterial species ( Streptococcus mutans [ P < 0.001] and Veillonella parvula [ P < 0.001]) and 3 health-associated bacterial species ( Corynebacterium durum [P = 0.008], Neisseria flavescens [ P = 0.005], and Streptococcus sanguinis [ P < 0.001]). Compared to the fluoride control dentifrice, the CPP-ACP dentifrice demonstrated significant differences for S. mutans ( P = 0.032), C. durum ( P = 0.007), and S. sanguinis ( P < 0.001), while combination CPP-ACP–cranberry dentifrice showed significant differences for S. mutans ( P < 0.001), V. parvula ( P < 0.001), N. flavescens ( P = 0.003), and S. sanguinis ( P < 0.001). However, no significant differences were observed in the bacterial load comparisons between the CPP-ACP and combination dentifrices for any of the targeted bacterial species ( P > 0.05). Conclusions: Overall, the results indicate that dentifrices containing CPP-ACP and polyphenol-rich cranberry extracts can influence a species-level shift in the ecology of the oral microbiome, resulting in a microbial community less associated with dental caries (Australian New Zealand Clinical Trial Registry ANZCTR 12618000095268). Knowledge Transfer Statement: The results of this randomized controlled trial indicate that dentifrices containing casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) and polyphenol-rich cranberry extracts were able to beneficially modulate the microbial ecology of dental plaque in a group of high caries-risk patients. This could contribute toward lowering the risk of developing new caries lesions, an important goal sought by patients, clinicians, and policy makers.

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Is there a role for casein phosphopeptide–amorphous calcium phosphate (cpp-acp) in paediatric dentistry?

Dental Update ◽

10.12968/denu.2021.48.2.127 ◽

2021 ◽

Vol 48 (2) ◽

pp. 127-132

Author(s):

Claire Warner ◽

Helen J Rogers

Keyword(s):

Calcium Phosphate ◽

Dental Caries ◽

Amorphous Calcium Phosphate ◽

Tooth Surface ◽

Orthodontic Appliances ◽

Dentine Hypersensitivity ◽

Carious Tooth ◽

Surface Loss ◽

Casein Phosphopeptide ◽

Structural Anomalies

In the age of minimally invasive dentistry, products with the ability to prevent and reverse common dental diseases are becoming increasingly popular. Casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) contains the compounds casein, phosphate and calcium, which all have the ability to remineralize enamel. Dental caries, visible demineralization following removal of orthodontic appliances, non-carious tooth surface loss and dentine hypersensitivity due to structural anomalies are frequently seen in children and young people. The evidence for CPP–ACP and fluoride-containing CPP–ACP is positive for managing dental caries and non-carious tooth surface loss, specifically erosion, but is less clear in the management of visible demineralization following orthodontic appliance removal and dentine hypersensitivity owing to structural anomalies. When recommending CPP–ACP-containing products, dental practitioners should be aware of medical and social contra-indications that preclude its use. CPD/Clinical Relevance: CPP–ACP may have a role in the management of common paediatric dental conditions.

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