Description of post-implantation embryonic stages in European roe deer (Capreolus capreolus ) after embryonic diapause

2017 ◽  
Vol 46 (6) ◽  
pp. 582-591
Author(s):  
M. Beyes ◽  
N. Nause ◽  
M. Bleyer ◽  
F.-J. Kaup ◽  
S. Neumann
Keyword(s):  
Roe Deer ◽  
Capreolus Capreolus ◽  
Embryonic Diapause ◽  
Post Implantation

scholarly journals Examination of Reproductive Performance of Roe Deer (Capreolus capreolus) in Hungary

2004 ◽  
pp. 33-38
Author(s):  
István Majzinger
Keyword(s):  
Reproductive Performance ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Tooth Wear ◽  
High Fertility ◽  
Previous Pregnancy ◽  
Embryo Number ◽  
The Difference ◽  
Post Implantation ◽  
Implantation Period

The objective of the research partly is to compare the reproduction performance of the populations living in different regions with regard to some special characteristics (age, condition).When estimating the age through tooth wear and cementum-layer-counting there was a difference of 0.87 years in favour of the first one (r=0,840; p<0,001). I found cementum layers at 42% of the does in the study after examining the MI teeth.There was lose connection between the weight (eviscerated, with head and legs) and the KFI (r=0,296; p<0,01), and for further analysis, I used only the KFI as the index for condition.The regional average KFI varied from 0.24-0.37 in fawns, 0.82-1.73 in does, with individual extremes of 0-4.05. Within the examined regions the highest index belonged to the prime-aged does, while the 1-year-olds had a lower rate, and it was the lowest in the does older than 8 years.The rate of fertility was between 83,3(ns)-100% as we can see from the presence of the CL. All the examined does were fertile, except in one region, while among the female fawns in two regions I only found three with active ovaries. The average number of CL was 1.5-2.13, and this varied by regions; all in all it was the highest in the 2-7-year-old group (1.96) and in the ones over 8 years (2.00!), while it was lower in the does younger than 1 year (1.90). The high fertility of the does over 8 years is remarkable.I could examine the number of embryos in two regions during the post-implantation period, and beside 100% fertility I found significant differences among the does, which can be associated with the condition. The ratio of CL carriers and the pregnant does was 100% and 73% in the two regions, the average number of CL were 1.92 and 1.72, while the average embryo number were 1.83 and 1.36 per doe. The difference between the CL and the embryo numbers on the two regions were 5% and 21%. The difference (prenatal loss) is in connection with the age (age class) of the doe. It is possible, however, that in some cases oestrus was not followed by gestation. But in roe deer, owing to the commonly known lack of luteolysis-mechanism (Flint et al., 1994), the regression of the CL of the does that did not get pregnant takes place in December and January, so the CL found in January cannot prove a previous pregnancy, which might have been followed by an abortion.Although it has to be proven, it seems that the number of the CL (potential progeny) can be associated with the age (r=0,418; p<0,01) and the weight (r=0,312; p<0,01) of the doe, while the embryo number (realised progeny) is influenced by the age of the doe and probably by external factors.It is essential to continue and extend the research to increase the reliability of the results and their correlation.

scholarly journals Progestogen Profiling Over the Course of Diapause and Resumption of Embryo Development in the European Roe Deer

2019 ◽  
Vol 1 (4) ◽  
pp. 158-167 ◽  
Author(s):  
Vera A. Van der Weijden ◽  
Anna-Katharina Hankele ◽  
Anna B. Rüegg ◽  
Marion Schmicke ◽  
Karoline Rehm ◽  
...  
Keyword(s):  
Embryo Development ◽  
Luteal Phase ◽  
Roe Deer ◽  
Binding Capacity ◽  
Mammalian Species ◽  
Capreolus Capreolus ◽  
Corpora Lutea ◽  
Embryonic Diapause ◽  
Weak Correlation ◽  
Species Specific

Progesterone (P4) plays a pivotal role in maintenance of pregnancy in many mammalian species. Species-specific P4 metabolites have been shown to function as primary acting progestogen and the receptor binding capacity varies between species. The European roe deer (Capreolus capreolus) displays a 4-5 month period of embryonic diapause, which decouples fertilization from implantation. The majority of roe deer have two corpora lutea that secrete P4. No changes in P4 concentrations have been observed during pre-implantation embryo development. As 5α-DHP is known to play a major role during pregnancy in elephants and horses, we hypothesized that 5α-DHP functions as additional progestogen facilitating embryo reactivation. The profile of 11 progestogens was quantified in roe deer plasma over the course of diapause and resumption of embryo development including P4, 3α- and 3β-DHP, 20α- and 20β-DHP, 5α- and 5β-DHP, 3α,5α- and 3α,5β-THP, as well as 3β,5α- and 3β,5β-THP. While P4 was most abundant during diapause and resumption of development, 20α-DHP was the most abundant P4 metabolite. This is different than in pregnant elephants, where 5α-DHP was most abundant, and the luteal phase in cattle, where 3α,5α-THP was most abundant. With the exception of a weak correlation of 3β,5α-THP, none of the progestogens significantly correlated with embryonic development in the roe deer. Thus, plasma 5α-DHP does not seem to play a role in embryo reactivation. We propose that progestogens might contribute to priming the endometrium for supporting embryo development and preparation for implantation.

scholarly journals Embryonic diapause in mammals and dormancy in embryonic stem cells with the European roe deer as experimental model

2021 ◽  
Vol 33 (2) ◽  
pp. 76
Author(s):  
Vera A. van der Weijden ◽  
Anna B. Rüegg ◽  
Sandra M. Bernal-Ulloa ◽  
Susanne E. Ulbrich
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Molecular Mechanisms ◽  
Roe Deer ◽  
Current Knowledge ◽  
Embryonic Stem ◽  
Capreolus Capreolus ◽  
Embryonic Diapause ◽  
Developmental Progression ◽  
Promising Application

In species displaying embryonic diapause, the developmental pace of the embryo is either temporarily and reversibly halted or largely reduced. Only limited knowledge on its regulation and the inhibition of cell proliferation extending pluripotency is available. In contrast with embryos from other diapausing species that reversibly halt during diapause, embryos of the roe deer Capreolus capreolus slowly proliferate over a period of 4–5 months to reach a diameter of approximately 4mm before elongation. The diapausing roe deer embryos present an interesting model species for research on preimplantation developmental progression. Based on our and other research, we summarise the available knowledge and indicate that the use of embryonic stem cells (ESCs) would help to increase our understanding of embryonic diapause. We report on known molecular mechanisms regulating embryonic diapause, as well as cellular dormancy of pluripotent cells. Further, we address the promising application of ESCs to study embryonic diapause, and highlight the current knowledge on the cellular microenvironment regulating embryonic diapause and cellular dormancy.

scholarly journals Conceptus-endometrial interactions and reproductive hormone profiles during embryonic diapause and reactivation of the blastocyst in the European roe deer (Capreolus capreolus)

Rangifer ◽  
1999 ◽  
Vol 19 (1) ◽  
pp. 41 ◽  
Author(s):  
Roger T. Lambert
Keyword(s):  
Protein Synthesis ◽  
De Novo ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Fold Increase ◽  
Secretory Proteins ◽  
Plasma Prolactin ◽  
Embryonic Diapause ◽  
Dimensional Electrophoresis

<p>Abstract: Roe deer blastocysts exhibit obligate embryonic diapause between early August and late December. The blastocyst then expands and elongates rapidly before implantation. The objective of this study was to ascertain the cues for reactivation of the diapausing blastocyst. Blood samples and reproductive tracts were collected from roe does during diapause, blastocyst expansion and subsequent implantation. Peripheral concentrations of oestradiol-17/3, progesterone and prolactin were measured by radioimmunoassay. Luteal progesterone release was determined following in vitro incubation. Conceptuses and endometrial tissue were cultured with 3H-leucine for 24 hours to measure de novo synthesis of secretory proteins. Endometrial secretory proteins were separated by two-dimensional electrophoresis. Results showed that peripheral progesterone concentrations declined by 55% just prior to expansion and did not rise until a 3-fold increase after implantation. Luteal progesterone release remained constant until expansion when it declined by 50% before increasing 2-fold at elongation and implantation. Concentrations of oestradiol-17/3 remained at a consistently low level during diapause and expansion until a 30-fold increase at elongation with concentrations remaining elevated after implantation. Plasma prolactin levels remained at basal concentrations during late diapause and then increased marginally at reactivation before decreasing again at elongation and implantation. Incorporation of radiolabel into both conceptus and endometrial secretory proteins was low during diapause, but incorporation in the conceptus increased 4&not;fold at expansion and by 24-fold at the expanded trophoblast stage. Incorporation into endometrial secretoty proteins remained constant until the expanded trophoblast stage and implantation when a 2-fold increase was recorded. Furthermore, the profile of endometrial secretory proteins was constant during diapause and expansion but changed qualitatively following implantation. These data indicate that both endometrial protein synthesis and sectetion did not change during late diapause and early expansion. The increase in conceptus protein synthesis not only precedes that of the endometrium but consistently low luteal progesterone release, peripheral progesterone and oestradioi-17/J concentrations at early expansion suggests that reactivation is not in response to a maternal uterine trigger.</p>

Enamel Thickness Differs between Field and Forest European Roe Deer Capreolus capreolus

2020 ◽  
Vol 68 (1) ◽  
pp. 100
Author(s):  
Jan Demesko ◽  
Marta Kurek ◽  
Patrycja Podlaszczuk ◽  
Janusz Markowski
Keyword(s):  
Roe Deer ◽  
Capreolus Capreolus ◽  
Enamel Thickness
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