miR‐27b‐5p inhibits BeWo cells fusion by regulating WNT2B and enzyme involved in progesterone synthesis

Ultrastructure of Choriocarcinoma in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006372x ◽

1969 ◽

Vol 27 ◽

pp. 362-363

Author(s):

John C. Garancis ◽

R. A. Pattillo

Keyword(s):

Cell Line ◽

Physiological Function ◽

Cell System ◽

Bewo Cell ◽

Bewo Cells ◽

Gestational Choriocarcinoma ◽

Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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Expression of interleukin-6 (IL-6), signal transducer and activator of transcription-3 (STAT-3) and telomerase in choriocarcinomas

Surgical and Experimental Pathology ◽

10.1186/s42047-020-00080-1 ◽

2020 ◽

Vol 3 (1) ◽

Author(s):

Luciana Pietro ◽

Fátima Bottcher-Luiz ◽

Lício Augusto Velloso ◽

Joseane Morari ◽

Marcelo Nomura ◽

...

Keyword(s):

Cell Transformation ◽

First Trimester ◽

Bewo Cell ◽

Placental Development ◽

Trimester Abortion ◽

Bewo Cells ◽

Culture Cells ◽

Bewo Cell Line ◽

Blastocyst Implantation ◽

First Trimester Of Pregnancy

Abstract Blastocyst implantation and neoplastic invasion have some common properties related to tissue invasion, mediated by various cytokines. Aim To compare the expression of IL-6, STAT-3 and telomerase in material of abortions in the first trimester of pregnancy, at term placentas and in choriocarcinomas. Methods Immunohistochemical reactions were performed on formalin fixed and included in paraffin samples from 3 groups: abortions, normal at term placentas and choriocarcinomas. Western Blot and Real-Time PCR assays were performed on fresh material from BeWo cell line and in primary culture cells of normal placenta. Results Immunohistochemical reactions: IL-6 expression was moderate in the first trimester abortion samples and high in at term placentas and choriocarcinomas. STAT-3 was strongly positive in all groups. Telomerase expression was absent in normal at term placentas but was increased in BeWo cells. Conclusion IL-6 and STAT-3 are present in the invasion process of the normal placental development and they are maintained during the malignant transformation to choriocarcinoma. The intense telomerase expression observed in BeWo cells was strongly associated with the malignant phenotype, confirming it as a good marker for cell transformation and tumor progression.

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