Difference in the seminal plasma protein expression in unexplained infertile men with successful and unsuccessful in vitro fertilisation outcome

Background and Aims: Azoospermia factor (AZF) region of the Ychromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequencies of Ychromosome microdeletions in azoospermic and oligospermic Iranian infertile men and compare them with other studies in different ethnic groups. Materials and Methods: At first, karyotype analysis was performed in 80 infertile men and 30 healthy age-matched counterparts as control group using standard cytogenetic methods. Second, genomic DNA was extracted from all cases and genetic screening was conducted for Y chromosome microdeletions by multiplex polymerase chain reaction for AZF genes on both infertile and control men using 6 STS markers on the long arm of the Y chromosome. Results: Totally, 49 infertile men were azoospermic and 31 were oligospermic. Y-chromosome microdeletions in the AZFc region were detected in 4 of azoospermic patients. Y-chromosome microdeletions was not detected in any of the oligospermic patients and the control group. Conclusions: This finding recommends that genetic counseling and screening before starting assisted reproductive techniques such as in vitro fertilisation and intracytoplasmic sperm injection can prevent unnecessary treatment and transmission of genetic defects to offspring

Download Full-text

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways

International Journal of Molecular Sciences ◽

10.3390/ijms21249342 ◽

2020 ◽

Vol 21 (24) ◽

pp. 9342

Author(s):

Patrycja Kurowska ◽

Ewa Mlyczyńska ◽

Anthony Estienne ◽

Alix Barbe ◽

Iwona Rajska ◽

...

Keyword(s):

Protein Expression ◽

Oocyte Maturation ◽

Control Level ◽

Cumulus Cells ◽

In Vitro Fertilisation ◽

Female Reproduction ◽

Dapi Staining ◽

Compound C ◽

In Vitro Oocyte Maturation

Oocyte maturation is a critical stage in embryo production and female reproduction. The aims of this study were to determine: (i) the mRNA and protein expression of vaspin and its receptor 78-kDa glucose-regulated (GRP78) in porcine cumulus–oocyte complexes (COCs) by real-time PCR and Western blot analysis, respectively, and their localisation by immunofluorescence; and (ii) the effects of vaspin on in vitro oocyte maturation (IVM) and the involvement of mitogen ERK1/2 (MAP3/1)- and AMPKα (PRKAA1)-activated kinases in the studied processes. Porcine COCs were matured in vitro for 22 h or 44 h with vaspin at a dose of 1 ng/mL and nuclear maturation assessed by Hoechst 33342 or DAPI staining and the measurement of progesterone (P4) level in the maturation medium. We showed that vaspin and GRP78 protein expression increased in oocytes and cumulus cells after IVM. Moreover, vaspin enhanced significantly porcine oocyte IVM and P4 concentration, as well as MAP3/1 phosphorylation, while decreasing PRKAA1. Using pharmacological inhibitors of MAP3/1 (PD98059) and PRKAA1 (Compound C), we observed that the effect of vaspin was reversed to the control level by all studied parameters. In conclusion, vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

Download Full-text

Characterization of the cDNA and in vitro expression of the ram seminal plasma protein RSVP14

Gene ◽

10.1016/j.gene.2013.02.016 ◽

2013 ◽

Vol 519 (2) ◽

pp. 271-278 ◽

Cited By ~ 6

Author(s):

Edith Serrano ◽

Rosaura Pérez-Pé ◽

Lucía Calleja ◽

Natalia Guillén ◽

Adriana Casao ◽

...

Keyword(s):

Plasma Protein ◽

Seminal Plasma ◽

In Vitro Expression

Download Full-text

Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction

Reproduction Fertility and Development ◽

10.1071/rd06101 ◽

2007 ◽

Vol 19 (1) ◽

pp. 103 ◽

Cited By ~ 69

Author(s):

S. S. Suarez

Keyword(s):

Assisted Reproduction ◽

Seminal Plasma ◽

Reproductive Tract ◽

Bos Taurus ◽

Female Reproductive Tract ◽

Surface Proteins ◽

In Vitro Fertilisation ◽

Storage Reservoir ◽

Bsp Proteins

Artificial insemination with sexed semen, in vitro fertilisation and intracytoplasmic sperm injection have been used to reproduce animals, but often not as successfully as natural mating. Learning more about how spermatozoa normally interact with the female tract can provide inspiration for developing improvements in assisted reproduction. The present review focuses on Bos taurus, because more is known about this species than others. At coitus, bull spermatozoa are deposited into the anterior vagina, where they rapidly enter the cervix. Cervical mucus quickly filters out seminal plasma from spermatozoa, unlike most assisted reproduction protocols. Spermatozoa that reach the uterus may require certain cell surface proteins to swim through the uterotubal junction. Shortly after passing through the junction, most spermatozoa are trapped in a storage reservoir by binding to oviducal epithelium, in the case of cattle via bovine seminal plasma (BSP) proteins coating the sperm head. As ovulation approaches, spermatozoa capacitate and shed BSP proteins. This reduces sperm binding to the epithelium and releases them from storage. Motility hyperactivation assists spermatozoa in leaving the storage reservoir, swimming through oviducal mucus and the cumulus oophorus, and penetrating the oocyte zona pellucida. Chemotactically regulated switching between asymmetrical (i.e. hyperactivated) and symmetrical flagellar beating may also guide spermatozoa to the oocyte.

Download Full-text

Effectiveness of seminal plasma in in vitro fertilisation treatment: a systematic review and meta‐analysis

BJOG An International Journal of Obstetrics & Gynaecology ◽

10.1111/1471-0528.15004 ◽

2017 ◽

Vol 126 (2) ◽

pp. 220-225 ◽

Cited By ~ 8

Author(s):

G Saccone ◽

A Di Spiezio Sardo ◽

A Ciardulli ◽

C Caissutti ◽

M Spinelli ◽

...

Keyword(s):

Systematic Review ◽

Seminal Plasma ◽

Meta Analysis ◽

In Vitro Fertilisation ◽

Fertilisation Treatment

Download Full-text

SELECTION OF INFERTILE MEN FOR IN-VITRO FERTILISATION

The Lancet ◽

10.1016/s0140-6736(84)91576-9 ◽

1984 ◽

Vol 324 (8412) ◽

pp. 1150-1151 ◽

Cited By ~ 5

Author(s):

George Rowland ◽

Jonathan Hewitt ◽

Jacques Cohen

Keyword(s):

In Vitro Fertilisation ◽

Infertile Men ◽

Selection Of

Download Full-text

Expression and potential biological role of α(1,2)fucosylated glycotopes on amniotic and seminal fibronectins

Biochemical Society Transactions ◽

10.1042/bst0390355 ◽

2011 ◽

Vol 39 (1) ◽

pp. 355-359 ◽

Cited By ~ 10

Author(s):

Iwona Kątnik-Prastowska ◽

Magdalena Orczyk-Pawiłowicz

Keyword(s):

In Vitro Fertilization ◽

Amniotic Fluid ◽

Seminal Plasma ◽

Potential Role ◽

Biological Role ◽

Vitro Fertilization ◽

Infertile Men

The present paper describes concisely the expression and role of α(1,2)-linked fucose on some glycoconjugates as well as the detection, distribution and potential role of that glycotope on human soluble plasma and cellular fibronectins in addition to the expression on both normal and pathological amniotic fluid and seminal plasma fibronectins. The determination of α(1,2)fucosylated glycans is considered with respect to its usefulness as a potential clinically applicable biomarker in obstetrics to monitor pregnancy and in andrology to evaluate the ejaculate of infertile men and in vitro fertilization.

Download Full-text