Intestinal microbiota in rainbow trout,Oncorhynchus mykiss, fed diets with different levels of fish-based and plant ingredients: A correlative approach with some plasma metabolites

2018 ◽  
Vol 24 (5) ◽  
pp. 1563-1576 ◽  
Author(s):  
F.-J. Gatesoupe ◽  
B. Fauconneau ◽  
C. Deborde ◽  
B. Madji Hounoum ◽  
D. Jacob ◽  
...  





2020 ◽  
Vol 142 ◽  
pp. 104060 ◽  
Author(s):  
Tania Pérez-Sánchez ◽  
Brenda Mora-Sánchez ◽  
Augusto Vargas ◽  
José Luis Balcázar


2004 ◽  
Vol 61 (7) ◽  
pp. 1247-1255 ◽  
Author(s):  
P Payan ◽  
H De Pontual ◽  
A Edeyer ◽  
G Borelli ◽  
G Boeuf ◽  
...  

This is the first study in which the effects of an external stress were analysed at different levels: plasma ho meostasis, endolymph chemistry, and otolith growth. Stress was applied to rainbow trout (Oncorhynchus mykiss) by exposure to Cl2 gas. In the plasma of Cl2-stressed trout, Na+ and Cl– decreased (70 mmol·L–1) and K+ increased (2.0 mmol·L–1), whereas total Ca was unchanged. A slight hypercapny (+2.4 mmHg (1 mmHg = 133.322 Pa)) was observed related to a significant rise (40%) in total CO2 without pH variation. In the endolymph, Na+ and Cl– also decreased, whereas other parameters (K+, PO43–, Mg, and, peculiarly, total Ca) remained stable. The important effect provoked by Cl2 stress in endolymph was an increase of proteins (factor of 2.6) and total CO2 (factor of 3.1) concentrations at the proximal side of the endolymph. The stress induced a decrease in otolith growth rate and produced a discontinuity (check) in the microstructure pattern of the otolith characterized by a large D zone. The variations in the endolymph composition are discussed and we propose that they result not only from changes in plasma concentrations, but also from changes (organic and crystallization) in otolith deposition.



Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 143 ◽  
Author(s):  
Simona Rimoldi ◽  
Elisabetta Gini ◽  
Federica Iannini ◽  
Laura Gasco ◽  
Genciana Terova

This study evaluated the effects of dietary insect meal from Hermetia illucens larvae on autochthonous gut microbiota of rainbow trout (Oncorhynchus mykiss). Three diets, with increasing levels of insect meal inclusion (10%, 20%, and 30%) and a control diet without insect meal were tested in a 12-week feeding trial. To analyze the resident intestinal microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used. The number of reads taxonomically classified according to the Greengenes database was 1,514,155. Seventy-four Operational Taxonomic Units (OTUs) at 97% identity were identified. The core of adhered intestinal microbiota, i.e., OTUs present in at least 80% of mucosal samples and shared regardless of the diet, was constituted by three OTUs assigned to Propiobacterinae, Shewanella, and Mycoplasma genera, respectively. Fish fed the insect-based diets showed higher bacterial diversity with a reduction in Proteobacteria in comparison to fish fed the fishmeal diet. Insect-meal inclusion in the diet increased the gut abundance of Mycoplasma, which was attributed the ability to produce lactic and acetic acid as final products of its fermentation. We believe that the observed variations on the autochthonous intestinal microbiota composition of trout are principally due to the prebiotic properties of fermentable chitin.









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