scholarly journals Intraocular lens surface roughness studied by atomic force microcopy in different environments in vitro

2018 ◽  
Vol 96 (S261) ◽  
pp. 61-61
2012 ◽  
Vol 38 (1) ◽  
pp. 146-154 ◽  
Author(s):  
Rupert W. Strauss ◽  
Markus E. Hochleitner ◽  
Wolfgang Gsenger ◽  
Claudia Alge-Priglinger ◽  
Christoph W. Faschinger ◽  
...  

2018 ◽  
Vol 12 (02) ◽  
pp. 176-183 ◽  
Author(s):  
Agda Marobo Andreotti ◽  
Cecília Alves De Sousa ◽  
Marcelo Coelho Goiato ◽  
Emily Vivianne Freitas da Silva ◽  
Cristiane Duque ◽  
...  

ABSTRACT Objective: The purpose of this study was to evaluate the influence of surface roughness in biofilm formation of four microorganisms (Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans) on acrylic resin surface of ocular prostheses. Materials and Methods: Acrylic resin samples were divided into six groups according to polishing: Group 1200S (1200 grit + silica solution); Group 1200; Group 800; Group 400; Group 120 and Group unpolished. Surface roughness was measured using a profilometer and surface images obtained with atomic force microscopy. Microbial growth was evaluated after 4, 24, and 48 hours of incubation by counting colony-forming units. Statistical Analysis Used: For roughness, it was performed 1-way ANOVA and parametric Tukey test α5% (P ≤ 0.05). For CFU data found, it was applied Kruskal-Wallis and Mann-Whitney tests. Results: Group 120 and 400 presented the highest roughness values. For S. epidermidis and S. aureus, Group 1200S presented the lowest values of microbial growth. For E. faecalis at 4 hour, microbial growth was not observed. C. albicans did not adhere to the acrylic resin. Except for Group 1200S, different surface roughnesses did not statistically interfere with microbial adhesion and growth on acrylic surfaces of ocular prostheses. Conclusions: The roughness did not interfere with the microbial adhesion of the microorganisms evaluated. The use of silica decreases significantly microbial growth.


2015 ◽  
Vol 41 (9) ◽  
pp. 1980-1988 ◽  
Author(s):  
Paloma Mazoteras ◽  
Ricardo Pedro Casaroli-Marano

2010 ◽  
Vol 4 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Luca Giacomelli ◽  
Giacomo Derchi ◽  
Andrea Frustaci ◽  
Orlando Bruno ◽  
Ugo Covani ◽  
...  

Background and Aims: Polishing may increase the surface roughness of composites, with a possible effect on bacterial growth and material properties. This preliminary in vitro study evaluates the effect of three different polishing systems (PoGo polishers, Enhance, Venus Supra) on six direct resin composites (Gradia Direct, Venus, Venus Diamond, Enamel Plus HFO, Tetric Evoceram, Filtek Supreme XT). Materials and Methods: For each composite, 12 square specimens were prepared: 9 specimens were polished, three for each different method, while three specimens were used as controls. Surface roughness was determined with AFM by measuring Root Mean Square (RMS) of average height. Results: PoGo polisher determined a significantly rougher surface, versus controls, in 5 out of 6 composites evaluated. Some significant differences from unpolished controls were observed also for Enhance polishing. Polishing with Venus Supra did not result in any significant difference in surface roughness versus controls. No differences were observed between different polishing systems. Conclusions: These preliminary results suggest that Venus Supra polishing system could determine a smoother composite surface if compared to the other polishing systems tested. On this basis, we are conducting an in vivo study to evaluate bacterial colonization on some combinations of composites and polishing protocols.


2006 ◽  
Vol 32 (8) ◽  
pp. 1378-1384 ◽  
Author(s):  
Marco Lombardo ◽  
Maria P. De Santo ◽  
Giuseppe Lombardo ◽  
Riccardo Barberi ◽  
Sebastiano Serrao

2015 ◽  
Vol 656-657 ◽  
pp. 63-67
Author(s):  
Ai Wen Tan ◽  
Belinda Pingguan-Murphy ◽  
Roslina Ahmad ◽  
Sheikh Akbar

In situ TiO2 nanofiber arrays have been successfully produced directly on a Ti-6Al-4V substrate by using thermal oxidation under a limited supply of oxygen. Their morphology, elemental composition, crystal structure, surface roughness and surface wettability were characterized by field-emission scanning electron microscope (FESEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffractometer (XRD), atomic force microscope (AFM) and contact angle goniometer, respectively. The results of material characterization studies revealed that TiO2 nanofibers possessed greater surface roughness and wettability, as well as the degree of crystallinity. In vitro characterization have also been evaluated by using bovine articular chondrocytes on the resulting TiO2 nanofibrous surface at different time points. Cell adhesion was observed qualitatively by using FESEM and cell proliferation was determined quantitatively by using AlamarBlue reduction assay. The results showed that the TiO2 nanofibrous substrate triggers enhanced chondrocytes adhesion, proliferation, and production of extracellular matrix (ECM) fibrils compared to untreated substrate. These results suggest that the oxidation process produces a surface structure to which chondrocytes affinity, and thus this surface would has potential use in implants designed for cartilaginous applications.


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