Retracted : Motility parameters, adenosine triphosphate content and oxidative stress indices of sterlet, Acipenser ruthenus sperm after 6 days of storage

2015 ◽  
Vol 48 (2) ◽  
pp. 719-724 ◽  
Author(s):  
Mohammad Sadegh Aramli ◽  
Hajar Azarin ◽  
Parham Farsi
2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.


2014 ◽  
Vol 7 (9) ◽  
pp. 689-692
Author(s):  
P. K. Rath ◽  
S. K. Panda ◽  
B. P. Mishra ◽  
R. C. Patra ◽  
I. Nath

2015 ◽  
Vol 25 (7) ◽  
pp. 507-513 ◽  
Author(s):  
Heshmat Sepehri Moghaddam ◽  
Saeed Samarghandian ◽  
Tahereh Farkhondeh

2015 ◽  
Vol 8 (3) ◽  
pp. 151-154 ◽  
Author(s):  
Saeed Samarghandian ◽  
Mohsen Azimi-Nezhad ◽  
Mahmoud M. Shabestari ◽  
Farahzad Jabbari Azad ◽  
Tahereh Farkhondeh ◽  
...  

Abstract Cadmium (Cd) is an environmental toxic metal implicated in lipid abnormalities. The present study was designed to elucidate the possible association between chronic exposure to Cd concentration and alterations in plasma lipid, lipoprotein, and oxidative stress indices in rats. Sixteen male rats were assigned to 2 groups of 8 rats each (test and control). The Cd-exposed group obtained drinking water containing cadmium chloride (CdCl2) in the concentration of 2.0 mg Cd/L in drinking water for 3 months. At the end of the experimental period, blood samples were obtained to determine the changes of serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), reduced glutathione (GSH), malondialdehyde (MDA) and also serum Cd contents. The results of the present study indicated that Cd administration significantly increased the serum levels of TG, TC, LDL-C, MDA and Cd with reduction in the HDL-C and GSH levels. In conclusion, evidence is presented that chronic exposure to low Cd concentration can adversely affect the lipid and lipoprotein profile via lipid peroxidation.


2012 ◽  
Vol 78 ◽  
pp. 22-27 ◽  
Author(s):  
Alina Kafel ◽  
Aleksandra Nowak ◽  
Jadwiga Bembenek ◽  
Joanna SzczygieŁ ◽  
MirosŁaw Nakonieczny ◽  
...  

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