Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

2021 ◽  
Author(s):  
Renata Catão Egger ◽  
Naiara Cristina Motta ◽  
Thales de Souza França ◽  
Alexmiliano Vogel Oliveira ◽  
Weslley Fernandes‐Braga ◽  
...  
Keyword(s):  
Reproductive Season ◽  
Sperm Cryopreservation ◽  
Prochilodus Lineatus

Acido delta-aminolevulínico dehidrasa (ALA-D) como biomarcador de exposición a plomo en peces

2008 ◽  
Author(s):  
◽  
Paula Edith Lombardi
Keyword(s):  
Cyprinus Carpio ◽  
Buenos Aires ◽  
Rio De La Plata ◽  
Río De La Plata ◽  
Prochilodus Lineatus ◽  
La Plata ◽  
Del Rio

La actividad de la enzima ácido δ-aminolevulínico dehidrasa (ALA-D) es un reconocido biomarcador específico de exposición a plomo. Debido a sus características ha sido utilizado en estudios de monitoreo ambiental para evaluar la exposición a plomo en diversos organismos, entre ellos peces. El principal objetivo del presente trabajo de tesis fue validar un método de evaluación general de riesgo ecotoxicológico por exposición a plomo en poblaciones naturales de peces. Para tal fin se seleccionaron tres especies recolectadas del río de la Plata: Prochilodus lineatus (sábalo), Leporinus obtusidens (boga) y Cyprinus carpio (carpa). Las zonas de muestreo elegidas fueron: Berazategui y Berisso ubicadas cerca de las emisiones de residuos cloacales provenientes de las ciudades de Buenos Aires y La Plata. La tercera, Atalaya, libre de emisiones cloacales, pero sujeta a emisiones diversas, todas ellas, de origen antropogénico. Los muestreos se realizaron entre junio de 2002 y mayo de 2004. En primera instancia se caracterizaron diversos parámetros de la enzima ALA-D en muestras de sangre y de tejido hepático. Los resultados obtenidos mostraron características similares en la enzima sanguínea de todas las especies estudiadas. Lo mismo se observó para la enzima hepática. La comparación entre tejidos mostró diferencias en el valor de Km, siendo menor para la enzima hepática. Posteriormente, se procedió a validar el uso de la enzima como parámetro biomarcador de exposición a plomo en estudios de campo.

Opportunities and challenges related to the implementation of sperm cryopreservation into breeding of salmonid fishes

2019 ◽  
Vol 132 ◽  
pp. 12-21 ◽  
Author(s):  
Sylwia Judycka ◽  
Joanna Nynca ◽  
Andrzej Ciereszko
Keyword(s):  
Salmonid Fishes ◽  
Sperm Cryopreservation

scholarly journals Expression of Apoptosis-Related Genes in Cat Testicular Tissue in Relation to Sperm Morphology and Seasonality—A Preliminary Study

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 489
Author(s):  
Sylwia Prochowska ◽  
Agnieszka Partyka ◽  
Wojciech Niżański
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Testicular Tissue ◽  
Reproductive Season ◽  
Tissue Samples ◽  
Protein Levels ◽  
Expression Of Genes ◽  
Testicular Regression ◽  
Preliminary Study ◽  
Apoptotic Genes

Apoptosis is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and testicular regression out of breeding season. The aim of this study was to assess if the expression of apoptosis-related genes in testicular tissue of domestic cats differed: (1) between normozoospermic and teratozoospermic donors, and (2) between reproductive and non-reproductive season. The expression of genes: BCL2L1, BCL2, BAX, BAD, FAS, FASLG, and caspases (CASP3, CASP8, CASP9, and CASP10) was analyzed by qRT-PCR in testicular tissue samples. During non-reproductive season significantly higher expression of two anti-apoptotic genes (BCL2L1 and BCL2) was observed. Additionally, there was a significant higher expression of CASP10 in teratozoospermic cats during non-reproductive than during reproductive season. No differences were noted between normozoospermic and teratozoospermic groups. Upregulation of some genes during the non-reproductive season indicates engagement of apoptotic mechanisms in the seasonal changes of semen quality in cats, however further studies on protein levels and analysis of changes on distinct testicular germinal layers are required. At the same time, teratozoospermia in the general population of cats seems to be not connected with dysregulation of apoptosis in the testes.

scholarly journals Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 203
Author(s):  
María Gemma Millán de la Blanca ◽  
Eva Martínez-Nevado ◽  
Cristina Castaño ◽  
Juncal García ◽  
Berenice Bernal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genetic Material ◽  
First Year ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
The Family ◽  
Second Year ◽  
American Flamingo ◽  
Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

Ivermectin: A multilevel approach to evaluate effects in Prochilodus lineatus (Valenciennes, 1836) (Characiformes, Prochilodontidae), an inland fishery species

2021 ◽  
pp. 149515
Author(s):  
Ismael Esteban Lozano ◽  
Yanina Grisel Piazza ◽  
Paola Babay ◽  
Emanuel Sager ◽  
Fernando Román de la Torre ◽  
...  
Keyword(s):  
Prochilodus Lineatus ◽  
Multilevel Approach ◽  
Fishery Species

scholarly journals Effects of Cryoprotective Medium Composition, Dilution Ratio, and Freezing Rates on Spotted Halibut (Verasper variegatus) Sperm Cryopreservation

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2153
Author(s):  
Irfan Zidni ◽  
Yun Ho Lee ◽  
Jung Yeol Park ◽  
Hyo Bin Lee ◽  
Jun Wook Hur ◽  
...  
Keyword(s):  
Previous Experiment ◽  
Activity Index ◽  
Medium Composition ◽  
Freezing Rate ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ringer’S Solution ◽  
Potential Market ◽  
Spotted Halibut ◽  
Cryopreservation Protocol

The spotted halibut is species that has a high potential market value in Korea, but the supply of seed is unstable because of the limited milt production of males. The objective of this research was to explore different aspects, such as CPAs, diluents, dilution ratio, and freezing rates, to develop an optimal sperm cryopreservation. The parameters assessed were movable sperm ratio, sperm activity index, survival rate, and DNA damage. The CPAs tested in this research were propylene glycol, dimethyl sulfoxide (DMSO), methanol, ethylene glycol, and glycerol. Different diluents, including 300 mM sucrose, 300 mM glucose, Stain’s solution, and Ringer’s solution, were investigated. The previous experiment showed that the optimal CPA for cryopreservation was DMSO with a concentration of 15% with 300 mM as diluent. To determine the effect of the dilution ratio, sperm was diluted to 1:1, 1:2, 1:10, 1:100, and 1:1000 with 300 mM sucrose containing DMSO at a final concentration of 15%. Lastly, the optimal freezing rate of the sperm was evaluated with four different freezing rates (−1, −5, −10, and −20 °C/min). Post-thaw sperm motility was higher with a dilution ratio lower than 1:2, and the freezing rate was less than −5 °C/min. In conclusion, these findings represent the development of a cryopreservation protocol for spotted halibut.

scholarly journals Differential Diagnosis of Azoospermia in Men with Infertility

2021 ◽  
Vol 10 (14) ◽  
pp. 3144
Author(s):  
Danilo L. Andrade ◽  
Marina C. Viana ◽  
Sandro C. Esteves
Keyword(s):  
Differential Diagnosis ◽  
Physical Examination ◽  
Detailed Analysis ◽  
Clinical Management ◽  
Semen Analysis ◽  
Testicular Biopsy ◽  
Nonobstructive Azoospermia ◽  
Sperm Cryopreservation ◽  
Imaging Studies ◽  
Detailed Medical History

The differential diagnosis between obstructive and nonobstructive azoospermia is the first step in the clinical management of azoospermic patients with infertility. It includes a detailed medical history and physical examination, semen analysis, hormonal assessment, genetic tests, and imaging studies. A testicular biopsy is reserved for the cases of doubt, mainly in patients whose history, physical examination, and endocrine analysis are inconclusive. The latter should be combined with sperm extraction for possible sperm cryopreservation. We present a detailed analysis on how to make the azoospermia differential diagnosis and discuss three clinical cases where the differential diagnosis was challenging. A coordinated effort involving reproductive urologists/andrologists, geneticists, pathologists, and embryologists will offer the best diagnostic path for men with azoospermia.

scholarly journals Land-snail eggs as a proxy of abrupt climatic cooling events during the reproductive season

2021 ◽  
Author(s):  
Fengjiang Li ◽  
Naiqin Wu ◽  
Yajie Dong ◽  
Dan Zhang ◽  
Yueting Zhang ◽  
...  
Keyword(s):  
Land Snail ◽  
Reproductive Season ◽  
Climatic Cooling

scholarly journals Microplastics and copper effects on the neotropical teleost Prochilodus lineatus: Is there any interaction?

2020 ◽  
Vol 242 ◽  
pp. 110659 ◽  
Author(s):  
Jéssica Fernanda Bernardes Roda ◽  
Mariana Machado Lauer ◽  
Wagner Ezequiel Risso ◽  
Claudia Bueno dos Reis Martinez
Keyword(s):  
Prochilodus Lineatus ◽  
Copper Effects
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