scholarly journals Unveiling epigenetic regulation in cancer, aging, and rejuvenation with in vivo reprogramming technology

2018 ◽  
Vol 109 (9) ◽  
pp. 2641-2650 ◽  
Author(s):  
Yuko Sogabe ◽  
Hiroshi Seno ◽  
Takuya Yamamoto ◽  
Yasuhiro Yamada
Keyword(s):  
Epigenetic Regulation

scholarly journals Antagonistic interaction between Ezh2 and Arid1a coordinates root patterning and development via Cdkn2a in mouse molars

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Junjun Jing ◽  
Jifan Feng ◽  
Jingyuan Li ◽  
Xia Han ◽  
Jinzhi He ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Root Development ◽  
Physiological Function ◽  
Tooth Root ◽  
Tooth Regeneration ◽  
Jaw Bones ◽  
Antagonistic Interaction ◽  
Tooth Type ◽  
Epigenetic Regulators

Patterning is a critical step during organogenesis and is closely associated with the physiological function of organs. Tooth root shapes are finely tuned to provide precise occlusal support to facilitate the function of each tooth type. However, the mechanism regulating tooth root patterning and development is largely unknown. In this study, we provide the first in vivo evidence demonstrating that Ezh2 in the dental mesenchyme determines patterning and furcation formation during dental root development in mouse molars. Mechanistically, an antagonistic interaction between epigenetic regulators Ezh2 and Arid1a controls Cdkn2a expression in the dental mesenchyme to regulate dental root patterning and development. These findings indicate the importance of balanced epigenetic regulation in determining the tooth root pattern and the integration of roots with the jaw bones to achieve physiological function. Collectively, our study provides important clues about the regulation of organogenesis and has general implications for tooth regeneration in the future.

scholarly journals The HDAC inhibitor valproate induces a bivalent status of the CD20 promoter in CLL patients suggesting distinct epigenetic regulation of CD20 expression in CLL in vivo

Oncotarget ◽  
2017 ◽  
Vol 8 (23) ◽  
pp. 37409-37422 ◽  
Author(s):  
Annarita Scialdone ◽  
Muhammad Sharif Hasni ◽  
Jesper Kofoed Damm ◽  
Andreas Lennartsson ◽  
Urban Gullberg ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Hdac Inhibitor ◽  
Cd20 Expression

scholarly journals A15 VITAMIN D DEFICIENCY PROMOTES INTESTINAL AUTOPHAGY DYSFUNCTION VIA EPIGENETIC REGULATION INVOLVING MIR142-3P IN VITRO AND IN VIVO

2018 ◽  
Vol 1 (suppl_2) ◽  
pp. 23-24
Author(s):  
D M Bronte-Tinkew ◽  
F Dang ◽  
A Hsieh ◽  
L H McGillis ◽  
I Verapalan ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Epigenetic Regulation

56 HBX BINDS IN VIVO ON THE HBV MINICHROMOSOME, MODIFIES THE EPIGENETIC REGULATION OF CCC-DNA FUNCTION AND POTENTIATES HBV REPLICATION

2008 ◽  
Vol 48 ◽  
pp. S25 ◽  
Author(s):  
L. Belloni ◽  
T. Poliicino ◽  
L. Cimino ◽  
G. Raffa ◽  
G. Raimondo ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Hbv Replication

Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

2005 ◽  
Vol 337 (3) ◽  
pp. 901-907 ◽  
Author(s):  
Niki Kourmouli ◽  
Yuh-man Sun ◽  
Sjaak van der Sar ◽  
Prim B. Singh ◽  
Jeremy P. Brown
Keyword(s):  
Epigenetic Regulation ◽  
Pericentric Heterochromatin

scholarly journals Functional in vivo characterization of zebrafish sox10 enhancers in melanoma and neural crest

2020 ◽  
Author(s):  
Rebecca L. Cunningham ◽  
Eva T. Kramer ◽  
Sophia K. DeGeorgia ◽  
Shayana Seneviratne ◽  
Vadim Grigura ◽  
...  
Keyword(s):  
Neural Crest ◽  
Epigenetic Regulation ◽  
High Specificity ◽  
Open Chromatin ◽  
Transcriptional Enhancer ◽  
Reporter Expression ◽  
Transgenic Lines ◽  
In Vivo Characterization

AbstractThe re-emergence of a neural crest transcriptional program, including Sox10 upregulation, is a key step in melanoma initiation in humans and zebrafish. We hypothesize that epigenetic regulation of sox10 modulates melanoma initiation. ATAC-Seq analysis of zebrafish melanoma tumors identifies recurrently open chromatin domains near sox10. Reporter constructs for each putative sox10 enhancer were examined in zebrafish embryos for neural crest activity and in stable transgenic lines for melanoma activity. One element, peak5 (23 kilobases upstream of sox10), drives EGFP reporter expression in a subset of neural crest cells, Kolmer-Agduhr neurons, and early melanoma patches and tumors with high specificity. A ∼200 bp region, conserved across the Cyprinidae family (fish), is required for peak5 activity in neural crest and melanoma, and contains dimeric SoxE binding sites essential for neural crest activity. Our work identifies a novel melanoma transcriptional enhancer, expanding our knowledge of epigenetic regulation of neural crest identity in melanoma.

scholarly journals Conservation of epigenetic regulation by the MLL3/4 tumour suppressor in planarian pluripotent stem cells

2017 ◽  
Author(s):  
Yuliana Mihaylova ◽  
Prasad Abnave ◽  
Damian Kao ◽  
Samantha Hughes ◽  
Alvina Lai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epigenetic Regulation ◽  
Cell System ◽  
Stem Cell Population ◽  
Loss Of Function ◽  
Tumour Suppressors ◽  
A Genome ◽  
Regulatory Effects

AbstractCurrently, little is known about the evolution of epigenetic regulation in animal stem cells. Using the planarian stem cell system to investigate the role of the COMPASS family of MLL3/4 histone methyltransferases, we demonstrate that their role as tumour suppressors in stem cells is conserved over a large evolutionary distance in animals. This also suggested the potential conservation of a genome wide epigenetic regulation program in animal stem cells, so we assessed the regulatory effects of Mll3/4 loss of function by performing RNA-seq and ChIP-seq on the G2/M planarian stem cell population, part of which contributes to the formation of outgrowths. We find many oncogenes and tumour suppressors among the affected genes that are therefore likely candidates for mediating MLL3/4 tumour suppression function in mammals, where little is known about in vivo regulatory targets. Our work demonstrates conservation of an important epigenetic regulatory program in animals and highlights the utility of the planarian model system for studying epigenetic regulation.

scholarly journals Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

2018 ◽  
Author(s):  
Christopher B. Mulholland ◽  
Franziska R. Traube ◽  
Edris Parsa ◽  
Eva-Maria Eckl ◽  
Maximillian Schönung ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Dna Demethylation ◽  
Epigenetic Marks

ABSTRACTThe TET-oxidized cytosine derivatives, 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), are considered DNA demethylation intermediates as well as stable epigenetic marks in mammals. We compared modified cytosine and enzyme levels in TET-knockout cells during naive pluripotency exit and found distinct and differentiation-dependent contributions of TET1 and TET2 to 5hmC and 5fC formation. The divergent modified cytosine levels argue for independent consecutive oxidation steps in vivo with broad implications for epigenetic regulation.

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