Amino acid metabolism-related gene expression-based risk signature can better predict overall survival for glioma

Abstract Background Welsh onion constitutes an important crop due to its benefits in traditional medicine. Nitrogen is an important nutrient for plant growth and yield; however, little is known about its influence on the mechanisms of Welsh onion regulation genes. In this study, we introduced a gene expression and amino acid analysis of Welsh onion treated with different concentrations of nitrogen (N0, N1, and N2 at 0 kg/ha, 130 kg/ha, and 260 kg/ha, respectively). Results Approximately 1,665 genes were differentially regulated with different concentrations of nitrogen. Gene ontology enrichment analysis revealed that the genes involved in metabolic processes, protein biosynthesis, and transportation of amino acids were highly represented. KEGG analysis indicated that the pathways were related to amino acid metabolism, cysteine, beta-alanine, arginine, proline, and glutathione. Differential gene expression in response to varying nitrogen concentrations resulted in different amino acid content. A close relationship between gene expression and the content of amino acids was observed. Conclusions This work examined the effects of nitrogen on gene expression and amino acid synthesis and provides important evidence on the efficient use of nitrogen in Welsh onion.

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Peer Review #2 of "Effects of sex and site on amino acid metabolism enzyme gene expression and activity in rat white adipose tissue (v0.1)"

10.7287/peerj.1399v0.1/reviews/2 ◽

2015 ◽

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Amino Acid ◽

Peer Review ◽

White Adipose Tissue ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Enzyme Gene ◽

Metabolism Enzyme ◽

Expression And Activity

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Maternal hypercortisolemia alters placental metabolism: a multiomics view

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00190.2020 ◽

2020 ◽

Vol 319 (5) ◽

pp. E950-E960

Author(s):

Serene Joseph ◽

Jacquelyn M. Walejko ◽

Sicong Zhang ◽

Arthur S. Edison ◽

Maureen Keller-Wood

Keyword(s):

Gene Expression ◽

Amino Acid ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Striated Muscle ◽

Late Pregnancy ◽

Late Gestation ◽

Isoleucine Leucine ◽

Altered Expression ◽

Fetal Serum

Previous studies have suggested that increases in maternal cortisol or maternal stress in late pregnancy increase the risk of stillbirth at term. In an ovine model with increased maternal cortisol over the last 0.20 of gestation, we have previously found evidence of disruption of fetal serum and cardiac metabolomics and altered expression of genes related to mitochondrial function and metabolism in biceps femoris, diaphragm, and cardiac muscle. The present studies were designed to test for effects of chronically increased maternal cortisol on gene expression and metabolomics in placentomes near term. We hypothesized that changes in placenta might underlie or contribute to the alterations in fetal serum metabolomics and thereby contribute to changes in striated muscle metabolism. Placentomes were collected from pregnancies in early labor (143 ± 1 days gestation) of control ewes ( n = 7) or ewes treated with cortisol (1 mg·kg−1·day−1 iv; n = 5) starting at day 115 of gestation. Transcriptomics and metabolomics were performed using an ovine gene expression microarray (Agilent 019921) and HR-MAS NMR, respectively. Multiomic analysis indicates that amino acid metabolism, particularly of branched-chain amino acids and glutamate, occur in placenta; changes in amino acid metabolism, degradation, or biosynthesis in placenta were consistent with changes in valine, isoleucine, leucine, and glycine in fetal serum. The analysis also indicates changes in glycerophospholipid metabolism and suggests changes in endoplasmic reticulum stress and antioxidant status in the placenta. These findings suggest that changes in placental function occurring with excess maternal cortisol in late gestation may contribute to metabolic dysfunction at birth.

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Embryonic genotype and inbreeding affect preimplantation development in cattle

Reproduction ◽

10.1530/rep-10-0282 ◽

2011 ◽

Vol 141 (5) ◽

pp. 625-632 ◽

Cited By ~ 18

Author(s):

G Lazzari ◽

S Colleoni ◽

R Duchi ◽

A Galli ◽

F D Houghton ◽

...

Keyword(s):

Gene Expression ◽

Amino Acid ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Blastocyst Stage ◽

Preimplantation Development ◽

Brown Swiss ◽

Molecular Features ◽

Cattle Herds

Infertility in cattle herds is a growing problem with multifactorial causes. Embryonic genotype and level of inbreeding are among the many factors that can play a role on reproductive efficiency. To investigate this issue, we produced purebred and crossbred bovine embryos by in vitro techniques from Holstein oocytes and Holstein or Brown Swiss semen and analyzed several cellular and molecular features. In the first experiment, purebred and crossbred embryos, obtained from abattoir oocytes, were analyzed for cleavage, development to morula/blastocyst stages, amino acid metabolism and gene expression of developmentally important genes. The results indicated significant differences in the percentage of compacted morulae, in the expression of three genes at the blastocyst stage (MNSOD, GP130 and FGF4) and in the utilization of serine, asparagine, methionine and tryptophan in day 6 embryos. In the second experiment, bovine oocytes were collected by ovum pick up from ten Holstein donors and fertilized with the semen of the respective Holstein sires or with Brown Swiss semen. The derived embryos were grown in vitro up to day 7, and were then transferred to synchronized recipients and recovered on day 12. We found that purebred/inbred embryos had lower blastocyst rate on days 7–8, were smaller on day 12 and had lower expression of the trophoblast gene PLAC8. Overall, these results indicate reduced and delayed development of purebred embryos compared with crossbred embryos. In conclusion, this study provides evidence that embryo genotype and high inbreeding can affect amino acid metabolism, gene expression, preimplantation development and therefore fertility in cattle.

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