An integrated framework using high‐dimensional mass cytometry and fluorescent flow cytometry identifies discrete B cell subsets in patients with red meat allergy

Purpose and appropriate sample typesThis 19-parameter, 18-colour flow cytometry panel was designed and optimised to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell and B-cell subsets within murine lymphoid tissues (Table 1). Cellular populations identified by employing this OMIP include 4 major subsets of B-cells (memory, activated, plasma cells and plasmablasts) and 7 major subsets of CD4+ T-cells (naïve, central memory, effector memory, helper, regulatory, follicular helper and follicular regulatory). Staining was performed on freshly isolated splenocytes from 21-day-old neonatal BALB/c mice, however due to the omission of mouse strain-specific markers, this OMIP can be implemented across a range of murine models where in-depth immunophenotyping of the diverse repertoire of T-cell and B-cell populations localised within lymphoid tissues is required.

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Identification of galactose-α-1,3-galactose in the gastrointestinal tract of the tickIxodes ricinus; possible relationship with red meat allergy

Allergy ◽

10.1111/all.12128 ◽

2013 ◽

Vol 68 (4) ◽

pp. 549-552 ◽

Cited By ~ 118

Author(s):

C. Hamsten ◽

M. Starkhammar ◽

T. A. T. Tran ◽

M. Johansson ◽

U. Bengtsson ◽

...

Keyword(s):

Gastrointestinal Tract ◽

Red Meat ◽

Meat Allergy ◽

Red Meat Allergy

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The red meat allergy syndrome in Sweden

Allergo Journal ◽

10.1007/s15007-016-1044-7 ◽

2016 ◽

Vol 25 (2) ◽

pp. 29-34 ◽

Cited By ~ 3

Author(s):

Danijela Apostolovic ◽

Thi Anh Thu Tran ◽

Maria Starkhammar ◽

Sara Sánchez-Vidaurre ◽

Carl Hamsten ◽

...

Keyword(s):

Red Meat ◽

Meat Allergy ◽

Red Meat Allergy

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Red meat allergy in Sweden: Association with tick sensitization and B-negative blood groups

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2013.07.050 ◽

2013 ◽

Vol 132 (6) ◽

pp. 1431-1434.e6 ◽

Cited By ~ 82

Author(s):

Carl Hamsten ◽

Thi Anh T. Tran ◽

Maria Starkhammar ◽

Annelie Brauner ◽

Scott P. Commins ◽

...

Keyword(s):

Red Meat ◽

Blood Groups ◽

Meat Allergy ◽

Red Meat Allergy

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The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy

Allergy ◽

10.1111/all.12948 ◽

2016 ◽

Vol 72 (2) ◽

pp. 266-273 ◽

Cited By ~ 18

Author(s):

D. Kollmann ◽

B. Nagl ◽

C. Ebner ◽

W. Emminger ◽

S. Wöhrl ◽

...

Keyword(s):

Red Meat ◽

Specific Antibodies ◽

Meat Allergy ◽

Red Meat Allergy

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Red meat allergy: clinical characteristics

Allergy Asthma & Respiratory Disease ◽

10.4168/aard.2020.8.3.142 ◽

2020 ◽

Vol 8 (3) ◽

pp. 142

Author(s):

Sejin Kim ◽

Jaechun Lee ◽

Ara Ko

Keyword(s):

Clinical Characteristics ◽

Red Meat ◽

Meat Allergy ◽

Red Meat Allergy

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A Case of α-Gal-Unrelated Red Meat-Induced Urticaria Treated by Omalizumab

Case Reports in Dermatology ◽

10.1159/000518421 ◽

2021 ◽

pp. 437-440

Author(s):

Makoto Kondo ◽

Yoshiaki Matsushima ◽

Shohei Iida ◽

Ai Umaoka ◽

Takehisa Nakanishi ◽

...

Keyword(s):

Chronic Urticaria ◽

Challenge Test ◽

Healthy Woman ◽

Food Challenge ◽

Red Meat ◽

Meat Allergy ◽

Red Meat Allergy ◽

History Of ◽

Food Challenge Test ◽

Repeatability Test

A 70-year-old healthy woman was referred to our hospital for chronic urticaria. She did not have a history of allergy, asthma, and rhinitis. She was initially diagnosed with α-gal-related urticaria based on an episode of delayed-type urticaria after eating red meat. The results of the intracutaneous allergen test for beef and pork were negative. Fluorenzyme immunoassays specific for IgE against α-gal, beef, and pork were also negative. She was diagnosed with an α-gal-unrelated red meat allergy following the reproduction of urticaria by a food challenge test. The patient was unresponsive to several drugs, including antihistamines or immunosuppressants. However, omalizumab administration suppressed her symptoms. Key Clinical Message: The diagnosis of red meat allergy may require a repeatability test by consuming red meat even though serum α-gal IgE antibody might be negative. The α-gal-unrelated red meat urticaria may be responsive to omalizumab.

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Red meat causing delayed anaphylactic airway edema: a case report

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20212135 ◽

2021 ◽

Vol 7 (6) ◽

pp. 1059

Author(s):

Aparna Das ◽

Saranya Thangavel ◽

Sauradeep Das ◽

Sunil Kumar Saxena

Keyword(s):

Indian Population ◽

Diagnostic Algorithm ◽

Red Meat ◽

Laryngeal Edema ◽

Meat Allergy ◽

Delayed Onset ◽

Red Meat Allergy ◽

Body Sensation ◽

Emergency Tracheostomy ◽

Life Threatening

The goals of emergency management of angioedema include prevention of spontaneous eruption, maintaining and securing the airway and to stop the progression of the disease. Laryngeal edema is one of the life-threatening complications of angioedema that can be managed by endotracheal intubation or emergency tracheostomy or cricothyrotomy. Recently, delayed onset food induced anaphylactic reactions are being recognised widely due to better clinical knowledge and technology that can substantiate the diagnosis. The classical finding of anaphylaxis to only proteins have been disproved and delayed onset food allergy (i.e.) 3-6 hours after ingestion of food has been attributed to specific carbohydrate moieties in glycolipids and glycoproteins such as Galactose-α-1,3-galactose found in red meat (beef, pork and lamb). Even though it is seen rarely in the Indian population, it should be a part of the diagnostic algorithm in order to prevent fatal complications. Hereby reporting 39 years old male with undiagnosed red meat allergy presented with features of foreign body sensation throat and laryngeal oedema and managed conservatively with steroids and nebulisation.

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Single-Cell Transcriptomic Analysis of Peripheral Blood Reveals a Novel B-Cell Subset in Renal Allograft Recipients With Accommodation

Frontiers in Pharmacology ◽

10.3389/fphar.2021.706580 ◽

2021 ◽

Vol 12 ◽

Author(s):

Quan Zhuang ◽

Hao Li ◽

Bo Peng ◽

Yang Liu ◽

Ying Zhang ◽

...

Keyword(s):

Flow Cytometry ◽

B Cell ◽

Single Cell ◽

Renal Allograft ◽

Immune Cell ◽

Nk Cell ◽

Esrd Patient ◽

Cell Subset ◽

Renal Transplant Recipients ◽

B Cell Subsets

Background: Kidney transplantation (KTx) is a preeminent treatment for end-stage renal disease (ESRD). After the application of immunosuppressants (IS), renal allograft recipients could reach a state called accommodation which means they are neither rejected nor infected. This study aimed to describe the details of this immune accommodation and reveal a novel mechanism of IS on immune cell subpopulations.Methods: We analyzed multiple cell subgroups and their gene expression of peripheral T, B, myeloid, and NK cells from renal allograft recipients with accommodation and healthy control (HC) by single-cell transcriptomics sequencing (scRNA-seq) and flow cytometry.Results: A total of 8,272 cells were isolated and sequenced from three individuals, including 2,758 cells from HC, 2,550 cells from ESRD patient, and 2,964 cells from KTx patient, as well as 396 immune response–related genes were detected during sequencing. 5 T-cell, 4 NK-cell, 5 myeloid, and 4 B-cell clusters were defined. Among them, a B-cell subset (CD19+IGLC3lowIGKChighTCL1A-CD127+) of renal transplant recipients with accommodation was significantly lower than that of HC and verified by flow cytometry, and this B-cell subset showed an activated potential because of its high expression of CD127. Furthermore, we found that IL32 might be the key cytokine to induce the differentiation of this B-cell cluster.Conclusion: We found a novel B-cell subset (CD19+IGLC3lowIGKChighTCL1A-CD127+) which was inhibited and decreased in renal allograft recipients with accommodation. This study might reveal the effect of commonly used IS in clinical practice on B-cell subsets and related mechanism.

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