Absolute quantification of Bet v 1 in birch polymerized allergenic extracts via mass spectrometry targeted analysis

2021 ◽  
Author(s):  
Raquel Moya ◽  
M. Antonia Odena ◽  
Mayte Gallego ◽  
Eliandre de Oliveira ◽  
Jerónimo Carnés
Keyword(s):  
Mass Spectrometry ◽  
Absolute Quantification ◽  
Bet V 1 ◽  
Targeted Analysis ◽  
Allergenic Extracts

High‐throughput, in‐depth and estimated absolute quantification of plasma proteome using data‐independent acquisition/mass spectrometry (“HIAP‐DIA”)

PROTEOMICS ◽  
2021 ◽  
pp. 2000264
Author(s):  
Yue Zhou ◽  
Zengqi Tan ◽  
Peng Xue ◽  
Yi Wang ◽  
Xiang Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Absolute Quantification ◽  
Plasma Proteome ◽  
Data Independent Acquisition ◽  
Using Data

scholarly journals Benchmarking Non-Targeted Metabolomics Using Yeast-Derived Libraries

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 160
Author(s):  
Evelyn Rampler ◽  
Gerrit Hermann ◽  
Gerlinde Grabmann ◽  
Yasin El Abiead ◽  
Harald Schoeny ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Protein Identification ◽  
Regulatory Elements ◽  
Yeast Fermentation ◽  
Compound Identification ◽  
Targeted Metabolomics ◽  
Targeted Analysis ◽  
Orthogonal State ◽  
Starting Point

Non-targeted analysis by high-resolution mass spectrometry (HRMS) is an essential discovery tool in metabolomics. To date, standardization and validation remain a challenge. Community-wide accepted cost-effective benchmark materials are lacking. In this work, we propose yeast (Pichia pastoris) extracts derived from fully controlled fermentations for this purpose. We established an open-source metabolite library of >200 identified metabolites based on compound identification by accurate mass, matching retention times, and MS/MS, as well as a comprehensive literature search. The library includes metabolites from the classes of (1) organic acids and derivatives (2) nucleosides, nucleotides, and analogs, (3) lipids and lipid-like molecules, (4) organic oxygen compounds, (5) organoheterocyclic compounds, (6) organic nitrogen compounds, and (7) benzoids at expected concentrations ranges of sub-nM to µM. As yeast is a eukaryotic organism, key regulatory elements are highly conserved between yeast and all annotated metabolites were also reported in the human metabolome database (HMDB). Orthogonal state-of-the-art reversed-phase (RP-) and hydrophilic interaction chromatography mass spectrometry (HILIC-MS) non-targeted analysis and authentic standards revealed that 104 out of the 206 confirmed metabolites were reproducibly recovered and stable over the course of three years when stored at −80 °C. Overall, 67 out of these 104 metabolites were identified with comparably stable areas over all three yeast fermentation and are the ideal starting point for benchmarking experiments. The provided yeast benchmark material enabled not only to test for the chemical space and coverage upon method implementation and developments but also allowed in-house routines for instrumental performance tests. Transferring the quality control strategy of proteomics workflows based on the number of protein identification in HeLa extracts, metabolite IDs in the yeast benchmarking material can be used as metabolomics quality control. Finally, the benchmark material opens new avenues for batch-to-batch corrections in large-scale non-targeted metabolomics studies.

Absolute Quantification of E1, Ubiquitin-Like Proteins and Nedd8–MLN4924 Adduct by Mass Spectrometry

2013 ◽  
Vol 67 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Xiaofeng Yang ◽  
James E. Brownell ◽  
Qing Xu ◽  
Fengying Zhu ◽  
Jingya Ma ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Absolute Quantification

scholarly journals Multiple Heart-Cutting Two Dimensional Liquid Chromatography And Isotope Dilution Tandem Mass Spectrometry For The Absolute Quantification Of Proteins In Human Serum

2021 ◽  
pp. 339022
Author(s):  
Amanda Suárez Fernández ◽  
Pablo Rodríguez-González ◽  
Lydia Álvarez ◽  
Montserrat García ◽  
Héctor González Iglesias ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Serum ◽  
Isotope Dilution ◽  
Absolute Quantification ◽  
Tandem Mass ◽  
Two Dimensional ◽  
The Absolute

Untargeted and targeted analysis of sarin poisoning biomarkers in rat urine by liquid chromatography and tandem mass spectrometry

2021 ◽  
Author(s):  
M. F. Vokuev ◽  
Т. М. Baygildiev ◽  
I. V. Plyushchenko ◽  
Y. A. Ikhalaynen ◽  
R. L. Ogorodnikov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Rat Urine ◽  
Targeted Analysis

Semi-targeted analysis of metabolites using capillary-flow ion chromatography coupled to high-resolution mass spectrometry

2011 ◽  
Vol 25 (22) ◽  
pp. 3447-3452 ◽  
Author(s):  
Karl Burgess ◽  
Darren Creek ◽  
Paul Dewsbury ◽  
Ken Cook ◽  
Michael P. Barrett
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Ion Chromatography ◽  
Capillary Flow ◽  
High Resolution Mass Spectrometry ◽  
Targeted Analysis ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals Ultraperformance Liquid Chromatography Isotope Dilution Tandem Mass Spectrometry for the Absolute Quantification of Proteins and Peptides

2008 ◽  
Vol 80 (19) ◽  
pp. 7649-7649
Author(s):  
Leah G. Luna ◽  
Tracie L. Williams ◽  
James L. Pirkle ◽  
John R. Barr
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Isotope Dilution ◽  
Absolute Quantification ◽  
Tandem Mass ◽  
The Absolute ◽  
Proteins And Peptides
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