Confirmation of association between Lewis blood group antigens and secretor status in pemphigus vulgaris

The classical paradigm of autoimmune pathogenesis involving specific genetic makeup and exposure to environmental triggers has been challenged recently by the addition of a third element, the loss of intestinal barrier function. Regardless of HLA B27 phenotype or gastrointestinal symptoms, evidence of ileitis, ileocolitis or colitis exists in patients with spondyloarthropathy. The FUT2 secretory gene is a strong candidate for Crohn's susceptibility by shaping the functional states of mucosal microbiota and may thus have influence on the release of zonulin, the main regulator of gut permeability. Gram negative bacteria precipitate and may be involved in the pathogenesis of spondyloarthropathies. Susceptibility to many infectious agents is associated with ABO blood group or secretor state. Patients who cannot secrete ABO and Lewis blood group antigens into body fluids, an ability controlled by a single gene on chromosome 19, are known to be at increased risk of certain autoimmune diseases associated with human leukocyte antigen (HLA) markers. Lewis (Le) blood group phenotype can be used to infer secretor status. The objective of this study was to determine the distribution of secretor state and Lewis blood group phenotype in patients with seronegative spondyloarthropathies and healthy control subjects. Hundred and ten (110) patients with seronegative spondyloarthropathies (58 females and 52 males) and 103 control (74 males and 29 females) subjects participated in this study. Samples of saliva and blood were subjected to haemagglutination inhibition tests for determination of secretor status and Lewis phenotype. A total of 92(84%) patients and 92 (89%) control subjects were secretors while 18 (16%) patients and 11 (11%) control subjects were non-secretors. There was no statistically significant difference (?2 1,461 p<0,05 and degrees of freedom 1) in distribution of secretor status in comparison to seronegative spondyloarthropathies by comparing two observed populations. Seven patients had modified (reduced) expression of Lewis b antigen on their erythrocytes. Reduction of Lewis b antigen expression was not observed on erythrocytes of healthy subjects. Reduced expression of Lewis b antigen could be a consequence of the inflammatory process within the gut and it also suggests several pathogenic mechanisms which may be relevant to the synthesis of Lewis antigens inside the gut or its absorption on erythrocytes in patients with spondyloarthropathy.

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Assessment of Lewis blood group antigens and secretor status in autopsy specimens

Forensic Science International ◽

10.1016/0379-0738(93)90221-u ◽

1993 ◽

Vol 61 (2-3) ◽

pp. 133-140 ◽

Cited By ~ 2

Author(s):

A. Busuttil ◽

C.C. Blackwell ◽

V.S. James ◽

D.A.C. MacKenzie ◽

A.T. Saadi ◽

...

Keyword(s):

Blood Group ◽

Blood Group Antigens ◽

Secretor Status ◽

Lewis Blood Group ◽

Autopsy Specimens

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Secretor Status of ABO Antigens in Saliva of a Defined Group of Iranian Patients with Pemphigus Vulgaris: A Case-Control Study

Scientifica ◽

10.1155/2020/2950856 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Sedigheh Bakhtiari ◽

Zahra Yadegari ◽

Marziyeh Kaviyani ◽

Zahra Namazi ◽

Mahin Bakhshi

Keyword(s):

Blood Group ◽

Case Control Study ◽

Pemphigus Vulgaris ◽

Case Control ◽

Blood Type ◽

Blood Group Antigens ◽

Secretor Status ◽

The People ◽

Significant Difference ◽

Control Study

Introduction. Pemphigus is a chronic inflammatory and autoimmune disease which can cause blisters and mucocutaneous erosions. ABO secretor refers to those who secrete ABO blood group antigens based on their blood type in body fluids such as saliva, sweat, tears, semen, and serum. Previous studies showed that nonsecretor people are more prone to certain autoimmune diseases. Aim. The aim of this study was to determine the ABO secretor status in the saliva of patients with pemphigus vulgaris. Materials and Methods. This case-control study was conducted on 35 patients with pemphigus vulgaris and 35 healthy controls. The two groups were matched for age and gender. Pemphigus vulgaris diagnosis was confirmed by histopathology and direct immunofluorescence microscopy. ABO blood grouping was done, and 5 ml of unstimulated saliva was collected to determine secretor status. Secretors were recognized from nonsecretors by the Wiener agglutination inhibition test. Results were extracted by using statistical chi-square and Fisher’s exact tests. Results. 16 male and 19 female patients aged 49.43 ± .12.37 years were compared with 16 male and 19 female controls aged 46.43 ± 11.88 years. The most frequent blood group among case and control groups was O (54.3% and 60%, respectively). There was no significant difference in blood groups (P=0.73). 90% of the samples were ABO secretors. The patient group included 31 (88.6%) and the control group included 32 (91.4%) ABO secretors; there was no significant difference between the two groups (P=1.000). Conclusion. In this study, we observed that the people with nonsecretor status in comparison with the people with secretor status are not more susceptible to develop pemphigus vulgaris.

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Association of Recurrent Candidal Vaginitis with Inheritance of Lewis Blood Group Antigens

The Journal of Infectious Diseases ◽

10.1093/infdis/172.6.1616 ◽

1995 ◽

Vol 172 (6) ◽

pp. 1616-1619 ◽

Cited By ~ 13

Author(s):

E. Hilton ◽

V. Chandrasekaran ◽

P. Rindos ◽

H. D. Isenberg

Keyword(s):

Blood Group ◽

Blood Group Antigens ◽

Lewis Blood Group

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THE HISTOLOGICAL DISTRIBUTION OF BLOOD GROUP SUBSTANCES A AND B IN MAN

Journal of Experimental Medicine ◽

10.1084/jem.111.6.785 ◽

1960 ◽

Vol 111 (6) ◽

pp. 785-800 ◽

Cited By ~ 297

Author(s):

Aron E. Szulman

Keyword(s):

Blood Group ◽

Fluorescent Antibody ◽

Sweat Glands ◽

Surgical Removal ◽

Positive Staining ◽

Fluorescent Antibody Technique ◽

Blood Group Antigens ◽

Specific Staining ◽

Antibody Technique ◽

Secretor Status

The mapping out of the histologic distribution of blood group antigens A and B in human tissues was performed by means of the fluorescent antibody technique. Human hyperimmune sera were conjugated with fluorescein isocyanate and applied to frozen sections of human material obtained at autopsy or after surgical removal. The material examined encompassed A, B, and AB subjects. In the latter the anti-A and the anti-B conjugate elicited the same picture. Group O tissues were used for controls and were uniformly negative. The secretor status of subjects was determined from the saliva or by the Lewis typing of erythrocytes. The results fall into the following main divisions: Endothelia of Vessels.—Widespread localization was demonstrated in the cell walls of endothelium of capillaries, veins, arteries, and of sinusoidal cells of spleen. Stratified Epithelia.—These showed good outlining of cells of the Malpighian (and the granular, when present) layers. In transitional epithelia, cells of the basal and contiguous layers gave specific staining. Mucus-Secreting Apparatus.—Positive staining was obtained in glands, goblet cells, and secreting surface epithelia. In non-secretors there was no identifiable antigen with the important exception of the deeper parts of gastric foveolae, deeper parts of crypts of Lieberkühn of bowel mucosa and Brunner's glands of the duodenum. Various Organs of Secretion and Excretion.—The pancreas (exocrine portion) and the sweat glands were found to produce the antigen irrespectively of secretor status. Breast, prostate, and endometrial glands on the other hand apparently secrete the antigen in conformity with the subject's secretor:non-secretor make-up. Thus the secretor:non-secretor status governs principally the antigens associated with mucous secretions and this in most but not all locations. The possible nature of this control is briefly discussed.

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