ADP ribosylation factor 1 facilitates spread of wheat dwarf virus in its insect vector

2019 ◽  
Vol 21 (9) ◽  
Author(s):  
Hui Wang ◽  
Yan Liu ◽  
Lu Zhang ◽  
Jiban Kumar Kundu ◽  
Wenwen Liu ◽  
...  
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Wheat Dwarf Virus ◽  
Adp Ribosylation ◽  
Adp Ribosylation Factor

A real-time loop-mediated isothermal amplification for detection of the wheat dwarf virus in wheat and the insect vector Psammotettix alienus

Plant Disease ◽  
2021 ◽  
Author(s):  
Xingan Hao ◽  
Licheng Wang ◽  
Xudong Zhang ◽  
Qinrong Zhong ◽  
Jamal U Ddin Hajano ◽  
...  
Keyword(s):  
Real Time ◽  
Lamp Assay ◽  
High Specificity ◽  
Positive Sample ◽  
Isothermal Amplification ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Wheat Dwarf Virus ◽  
Loop Mediated Isothermal Amplification ◽  
Time Loop

Wheat dwarf virus (WDV, genus Mastrevirus, family Geminiviridae) is an economically important and widespread pathogen of cereal crops. It causes huge yield loss in wheat due to the unavailability of resistant varieties and rapid transmission by the vector leafhopper, Psammotettix alienus (Dahlb). To monitor and forecast this viral disease, an early diagnosis method is required for WDV detection in both infected plants and the virus vectors. In this study, we developed a real-time loop-mediated isothermal amplification (LAMP) assay for WDV detection. The positive sample could be detected within 28-32 min by following a simple and cost-effective procedure. The real-time LAMP assay showed a sensitivity of 2.7×105-6 copies/µL for detection and a high specificity for WDV amplification, with a similar accuracy to qPCR. Furthermore, a tube-closed dye method facilitates the inspection of the LAMP reaction and avoids cross contamination in the detection of the virus. This valuable detection assay could serve as an important tool for diagnosis and forecasting wheat dwarf disease intensity in the field.

Expression of engineered wheat dwarf virus in seed-derived embryos

1990 ◽  
Vol 79 (1) ◽  
pp. 158-162
Author(s):  
R. Topfer ◽  
B. Gronenborn ◽  
S. Schaefer ◽  
J. Schell ◽  
H.-H. Steinbiss
Keyword(s):  
Dwarf Virus ◽  
Wheat Dwarf Virus

ADP-ribosylation factor-like 8b is required for the development of mouse models of systemic lupus erythematosus

2019 ◽  
Vol 31 (4) ◽  
pp. 225-237
Author(s):  
Shin-Ichiroh Saitoh ◽  
Yoshiko Mori Saitoh ◽  
Kenji Kontani ◽  
Katsuaki Sato ◽  
Kensuke Miyake
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Mouse Models ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Adp Ribosylation ◽  
Adp Ribosylation Factor

scholarly journals Characterization of a glucose-repressible ADP-ribosylation factor 3 (ARF3) from Saccharomyces cerevisiae.

1994 ◽  
Vol 269 (33) ◽  
pp. 20931-20937
Author(s):  
F.J. Lee ◽  
L.A. Stevens ◽  
Y.L. Kao ◽  
J. Moss ◽  
M. Vaughan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Adp Ribosylation ◽  
Adp Ribosylation Factor

scholarly journals Characterization of the human gene encoding ADP-ribosylation factor 1, a guanine nucleotide-binding activator of cholera toxin.

1992 ◽  
Vol 267 (13) ◽  
pp. 9028-9034
Author(s):  
C.M. Lee ◽  
R.S. Haun ◽  
S.C. Tsai ◽  
J Moss ◽  
M Vaughan
Keyword(s):  
Cholera Toxin ◽  
Human Gene ◽  
Nucleotide Binding ◽  
Guanine Nucleotide ◽  
Gene Encoding ◽  
Adp Ribosylation ◽  
Guanine Nucleotide Binding ◽  
Adp Ribosylation Factor
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