Reactive dyes for living cells: Applications, artefacts, and some comparisons with textile dyeing

2021 ◽  
Author(s):  
Richard W. Horobin ◽  
Juan C. Stockert ◽  
Hua Zhang
2007 ◽  
Vol 75 (3) ◽  
pp. 647-652 ◽  
Author(s):  
Lluís Núñez ◽  
José Antonio García-Hortal ◽  
Francesc Torrades

2017 ◽  
Vol 142 ◽  
pp. 1397-1405 ◽  
Author(s):  
Aurora Turcanu ◽  
Thomas Bechtold
Keyword(s):  

2016 ◽  
Vol 6 (1) ◽  
pp. 67 ◽  
Author(s):  
Borislav N. Malinovic ◽  
Miomir G. Pavlovic

<span lang="EN-US">The textile dyeing industry consumes large quantities of water and produces large volumes of wastewater from different steps in the dyeing and finishing processes. Wastewater from printing and dyeing units is often rich in color, containing residues of reactive dyes and chemicals, such as complex components. This study investigates the decolorization of synthetic dye wastewater containing textile dye Reactive Violet 5 (RV5) by electrocoagulation. A laboratory batch reactor was used to investigate the effect of various operating parameters using aluminium (Al), iron (Fe) and stainless steel (SS) anode. The effect of dye concentration, current density, supporting electrolyte, sup­porting electrolyte concentration, electrolysis duration, and material of anode of the systems were evaluated. Color removal efficiency was 22, 91.5 and 99.8 % in 15 minutes using Al, Fe and SS anode, respectively (j = 10 mA/cm<sup>2</sup>, c<sub>NaCl </sub>= 0.171 M).</span>


1999 ◽  
Author(s):  
◽  
Ayesha Hansa

An environmental problem facing the textile industry is the coloured effluent from the dyeing of cellulosic fibres with reactive dyes. Reactive dye loss during dyeing operations is about 10 to 40 %, indicating the need to learn more about the fate of these dyes. Increasing environmental regulations are driving technical innovation to manage this problem. Good analytical techniques for the separation and detection of reactive dyes and their derivatives are necessary for monitoring dye-house effluent, as well as in the optimisation of dye synthesis, purification, formulation and application.


2013 ◽  
Vol 5 ◽  
pp. 279-282 ◽  
Author(s):  
Suphitcha Wijannarong ◽  
Sayam Aroonsrimorakot ◽  
Patana Thavipoke ◽  
charaporn Kumsopa ◽  
Suntree Sangjan

Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.


Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.


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