The most important factor in the intensification and rise of the production of the cotton industry in Tajikistan is the selection of new varieties characterized by high yield and its quality. The increase in the production of the cotton industry can be achieved not by expansion of acreage, since this possibility is very limited in a mountainous country, but by breeding new varieties of cotton and spreading them into production. The use of photosynthetic test characteristics in breeding contributes to the process of creating new varieties that meet the demand of agricultural production and the textile industry, i.e. varieties with a complex of economically useful features — precocity, high productivity, resistance to diseases and pests, optimal adaptive properties, characterized by high fiber yield and its technological quality. By methods of hybridization and selection by offspring on the basis of photosynthetic test characteristics from domestic and foreign variety samples, 28 introgressive genotypes were created that are characterized by complex of economically useful traits. It was found that out of the 28 genotypes of medium-fiber cotton studied by us, according to the main indicators of productivity — the number of full-fledged boxes per plant, the mass of raw cotton of one box and the yield — 6 genotypes were notable. The number of boxes per plant in the most productive was 12–15 pieces, the mass of raw cotton of one box is up to 6.6 g, and the yield of raw cotton is from 71.5 to 93.0 g/plant, which is significantly higher than in the standard Hisor variety.