Histopathology‐guided mass spectrometry differentiates benign nevi from malignant melanoma

2019 ◽  
Vol 47 (3) ◽  
pp. 226-240 ◽  
Author(s):  
Rossitza Lazova ◽  
Katy Smoot ◽  
Heather Anderson ◽  
Matthew J. Powell ◽  
Arlene S. Rosenberg ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Malignant Melanoma ◽  
Benign Nevi

scholarly journals Imaging Mass Spectrometry-Based Proteomic Analysis to Differentiate Melanocytic Nevi and Malignant Melanoma

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3197
Author(s):  
Rita Casadonte ◽  
Mark Kriegsmann ◽  
Katharina Kriegsmann ◽  
Isabella Hauk ◽  
Rolf R. Meliß ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Malignant Melanoma ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Imaging Mass Spectrometry ◽  
Independent Set ◽  
Molecular Techniques ◽  
Charge Ratio ◽  
Linear Discriminant ◽  
Melanocytic Nevi

The discrimination of malignant melanoma from benign nevi may be difficult in some cases. For this reason, immunohistological and molecular techniques are included in the differential diagnostic toolbox for these lesions. These methods are time consuming when applied subsequently and, in some cases, no definitive diagnosis can be made. We studied both lesions by imaging mass spectrometry (IMS) in a large cohort (n = 203) to determine a different proteomic profile between cutaneous melanomas and melanocytic nevi. Sample preparation and instrument setting were tested to obtain optimal results in term of data quality and reproducibility. A proteomic signature was found by linear discriminant analysis to discern malignant melanoma from benign nevus (n = 113) with an overall accuracy of >98%. The prediction model was tested in an independent set (n = 90) reaching an overall accuracy of 93% in classifying melanoma from nevi. Statistical analysis of the IMS data revealed mass-to-charge ratio (m/z) peaks which varied significantly (Area under the receiver operating characteristic curve > 0.7) between the two tissue types. To our knowledge, this is the largest IMS study of cutaneous melanoma and nevi performed up to now. Our findings clearly show that discrimination of melanocytic nevi from melanoma is possible by IMS.

scholarly journals Endogenous expression mapping of malignant melanoma by mass spectrometry imaging

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Yutaka Sugihara ◽  
Daniel Rivas ◽  
Johan Malm ◽  
Marcell Szasz ◽  
HoJeong Kwon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Malignant Melanoma ◽  
Mass Spectrometry Imaging ◽  
Endogenous Expression

Diagnostic Distinction of Malignant Melanoma and Benign Nevi by a Gene Expression Signature and Correlation to Clinical Outcomes

2017 ◽  
Vol 26 (7) ◽  
pp. 1107-1113 ◽  
Author(s):  
Jennifer S. Ko ◽  
Balwir Matharoo-Ball ◽  
Steven D. Billings ◽  
Brian J. Thomson ◽  
Jean Y. Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Malignant Melanoma ◽  
Clinical Outcomes ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Benign Nevi

scholarly journals Quantitative label-free mass spectrometry analysis of formalin-fixed, paraffin-embedded tissue representing the invasive cutaneous malignant melanoma proteome

2016 ◽  
Vol 12 (5) ◽  
pp. 3296-3304 ◽  
Author(s):  
Paul Dowling ◽  
Benvon Moran ◽  
Edel McAuley ◽  
Paula Meleady ◽  
Michael Henry ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Malignant Melanoma ◽  
Cutaneous Malignant Melanoma ◽  
Mass Spectrometry Analysis ◽  
Label Free ◽  
Spectrometry Analysis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Development and validation of a gene expression signature to distinguish malignant melanoma from benign nevi.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. 9021-9021
Author(s):  
Colleen Rock ◽  
Loren E Clarke ◽  
M. Bryan Warf ◽  
Darl D Flake ◽  
Anne-Renee Hartman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Malignant Melanoma ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Development And Validation ◽  
Benign Nevi

scholarly journals Mass Spectrometry Imaging Can Distinguish on a Proteomic Level Between Proliferative Nodules Within a Benign Congenital Nevus and Malignant Melanoma

2017 ◽  
Vol 39 (9) ◽  
pp. 689-695 ◽  
Author(s):  
Rossitza Lazova ◽  
Zhe Yang ◽  
Constantin El Habr ◽  
Young Lim ◽  
Keith Adam Choate ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Malignant Melanoma ◽  
Mass Spectrometry Imaging ◽  
Congenital Nevus

Metastatic Malignant Melanoma in Prepubertal Children

PEDIATRICS ◽  
1975 ◽  
Vol 55 (2) ◽  
pp. 191-204
Author(s):  
Daniel J. Trozak ◽  
Willard D. Rowland ◽  
Funan Hu
Keyword(s):  
Malignant Melanoma ◽  
Metastatic Melanoma ◽  
Correct Diagnosis ◽  
Metastatic Malignant Melanoma ◽  
Epithelioid Cell ◽  
Low Grade ◽  
Microscopic Appearance ◽  
Malignant Melanomas ◽  
Real Nature ◽  
Benign Nevi

Medical literature is full of involved and Confusing reports on the genesis, incidence, and development of metastatic melanoma in children. The reason for this confusion can be found in the early uncertainties that surrounded the real nature and correct diagnosis of metastatic melanoma in the prepubertal child. Before 1950, reports of melanomas with metastases in children were rare, poorly documented, and in many cases erroneous.1-4 In particular, benign nevi (now known as benign juvenile melanoma) went unrecognized and because of their alarming microscopic features were often diagnosed as malignant melanomas. For example, deceived by the predilection of this nevus for childhood, its frightening appearance, and failure to metastasize, Pack and Anglem wrote: ". . . . although malignant melanomas are found in infancy and childhood, they are of low grade malignancy and seldom metastasize" In a later paper, Pack6 coined the term "prepubertal melanoma" for those nonmetastasizing pigmented tumors of children which were microscopically indistinguishable from melanoma. He suggested removal before puberty when endocrinologic stimulation rendered certain of them capable of metastasis. Other investigators also noted this seeming disparity between microscopic appearance and clinical behavior.7 In 1948, Spitz8 provided the criteria for separating this unusual-looking nevus from malignant melanoma and termed it "juvenile melanoma." Later she and Allen9 showed that although these lesions are not restricted to children, they are much more common before puberty.* Subsequent authors10-13 have also classified juvenile melanoma among the benign nevi and the term spindle or epithelioid cell nevus10,12,13 is now preferred. McWhorter and Woolner13 in 1954 reviewed the subject of malignant melanoma in children and suggested that the favorable prognosis was spurious and due to the erroneous classification of spindle and epithelioid cell nevi under the former agnosis.

Expression of ZO Proteins in Benign Nevi and Skin Malignant Melanoma

2020 ◽  
Vol 46 (2) ◽  
pp. e134-e135
Author(s):  
Eleftheria Lakiotaki ◽  
Irakles Delicostantinou ◽  
Neofytos Basios ◽  
Antonis Tsimpoukelis ◽  
Georgios Chelidonis ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Benign Nevi
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