The role of long noncoding RNA THAP9‐AS1 in the osteogenic differentiation of dental pulp stem cells via the miR‐652‐3p/VEGFA axis

Human dental pulp stem cells (hDPSCs) are capable of forming mineralized nodules. The proliferation and osteogenic differentiation of hDPSCs are very important for alleviating tooth defects caused by related diseases. Angel-ica polysaccharide (ASP) is the main bioactive ingredient extracted from the angelica root. ASP has a variety of biological functions, including immune regulation, antitumor activity, and hematopoiesis. However, its possible effects on hDPSCs are still unclear. In this study, we aimed to investigate the role of ASP in periodontal diseases. We found that ASP promoted the proliferation of hDPSCs and osteogenic differentiation of hDPSCs. We further found that it promoted the expression of osteogenic-related genes, including ALP, RUNX2, Col1a1, and OCN. Mechanically, we found that ASP activated the Wnt/β-catenin pathway. In conclusion, our results suggested that ASP promoted the proliferation and osteogenic differentiation of hDPSCs via the Wnt/β-catenin pathway.

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Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells

Bioactive Materials ◽

10.1016/j.bioactmat.2021.01.020 ◽

2021 ◽

Vol 6 (9) ◽

pp. 2742-2751

Author(s):

Myung Chul Lee ◽

Hoon Seonwoo ◽

Kyoung Je Jang ◽

Shambhavi Pandey ◽

Jaewoon Lim ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Gene Carrier ◽

Novel Gene ◽

Equine Bone

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Chrysin induces osteogenic differentiation of human dental pulp stem cells

Experimental Cell Research ◽

10.1016/j.yexcr.2020.112466 ◽

2021 ◽

Vol 400 (2) ◽

pp. 112466

Author(s):

J.F. Huo ◽

M.L. Zhang ◽

X.X. Wang ◽

D.H. Zou

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.06.101 ◽

2021 ◽

Author(s):

Shilpa Bhandi ◽

Ahmed Alkahtani ◽

Mohammed Mashyakhy ◽

Hosam Ali Baeshen ◽

Mohammed Mustafa ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Optimal Conditions

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Involvement of the long noncoding RNA H19 in osteogenic differentiation and bone regeneration

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02149-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Zimo Zhou ◽

Mohammad Showkat Hossain ◽

Da Liu

Keyword(s):

Bone Regeneration ◽

Osteogenic Differentiation ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Osteoblast Differentiation ◽

Osteoclast Differentiation ◽

Complex Processes ◽

Osteogenic Induction ◽

Novel Target

AbstractOsteogenic differentiation and bone regeneration are complex processes involving multiple genes and multiple steps. In this review, we summarize the effects of the long noncoding RNA (lncRNA) H19 on osteogenic differentiation.Osteogenic differentiation includes matrix secretion and calcium mineralization as hallmarks of osteoblast differentiation and the absorption of calcium and phosphorus as hallmarks of osteoclast differentiation. Mesenchymal stem cells (MSCs) form osteoprogenitor cells, pre-osteoblasts, mature osteoblasts, and osteocytes through induction and differentiation. lncRNAs regulate the expression of coding genes and play essential roles in osteogenic differentiation and bone regeneration. The lncRNA H19 is known to have vital roles in osteogenic induction.This review highlights the role of H19 as a novel target for osteogenic differentiation and the promotion of bone regeneration.

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