scholarly journals PM 7/129 (2) DNA barcoding as an identification tool for a number of regulated pests

EPPO Bulletin ◽  
2021 ◽  
Vol 51 (1) ◽  
pp. 100-143
Keyword(s):  
Dna Barcoding ◽  
Identification Tool

Diagnostic and phylogenetic utility of the first DNA barcode library for longhorn beetles (Coleoptera: Cerambycidae) from the Russian Far East

Zootaxa ◽  
2017 ◽  
Vol 4276 (3) ◽  
pp. 441 ◽  
Author(s):  
VASILY V. GREBENNIKOV ◽  
EDUARD JENDEK ◽  
MAXIM ED. SMIRNOV
Keyword(s):  
Dna Barcoding ◽  
Phylogenetic Signal ◽  
Russian Far East ◽  
Far East ◽  
Dna Barcode ◽  
Longhorn Beetles ◽  
Gene Coi ◽  
Online Library ◽  
Identification Tool ◽  
The Russian Far East

One hundred forty two specimens representing 56 species of longhorn beetles (Cerambycidae) from the Russian Far East were sequenced for a 658 bp fragment of the 5' end of the mitochondrial cytochrome oxidase subunit I gene (COI, =DNA barcode). The data are publicly available in an open access online library (dx.doi.org/10.5883/DS-CERRF). All analysed species could be differentiated using the standard online DNA barcode identification tool, except for a group of three sympatric colour-defined Menesia species. Seven Menesia records share the same Barcode Identification Number (=BIN), while the single specimen of M. flavotecta shares the same haplotype as some of M. sulphurata. Excluding the Menesia case, the Barcode Index Numbers (BINs) uniquely correspond to the analysed species, in all but the four specimens of Chlorophorus simillimus where they share two BINs. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data and in the Maximum Likelihood analysis, all four subfamilies were recovered as monophyletic. Notwithstanding the few detected deviations from the absolute taxonomic/phylogenetic match, DNA barcoding is a powerful identification tool with a capacity to place an undocumented record among its closest relatives. 

scholarly journals Species Identification of Marine Fishes in China with DNA Barcoding

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Junbin Zhang
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
High Efficiency ◽  
Introgressive Hybridization ◽  
Genetic Distances ◽  
Gene Coi ◽  
Interspecific Comparisons ◽  
Mitochondrial Cytochrome Oxidase ◽  
Two Parameter ◽  
Identification Tool

DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the generaPampusin which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.

scholarly journals Chemistry and Molecular Characteristics of Premna flavescens Wall. ex C. B. Clarke in Vietnam

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110314
Author(s):  
Le Minh Ha ◽  
Ngo Thi Phuong ◽  
Nguyen Thi Phuong Trang ◽  
Dinh Thi Thu Thuy ◽  
Le Ngoc Hung
Keyword(s):  
Essential Oil ◽  
Dna Barcoding ◽  
Species Identification ◽  
Folk Medicine ◽  
Molecular Characteristics ◽  
The Family ◽  
First Time ◽  
Identification Tool ◽  
Inflammatory Effect

Premna flavescens Wall. ex C. B. Clarke, a medicinal plant in the family Lamiaceae, was used for the treatment of arthritis in folk medicine. From the essential oil extracted from the leaves of this plant we have identified 51 components, with β-caryophyllene as the major component (26.3%). The essential oil showed a strong anti-inflammatory effect in vitro with an IC50 of 5.88 µg/mL. DNA barcoding is an useful tool for species identification based on the standardized genomic DNA fragments, but the molecular database of P flavescens is still lacking from Genebank. A molecular species identification tool for P flavescens was developed for the first time using DNA barcoding. The sequences of rbcL, trnH–psbA, internal transcribed spacer, and 18S barcodes of P flavescens were submitted to the Genebank with the accession numbers MW553265, MW553266, MT935698.1, and MW485128.1, respectively. This will be highly useful for the molecular authentication of the marker samples.

scholarly journals African freshwater eel species (Anguilla spp.) identification through DNA barcoding

2020 ◽  
Vol 71 (11) ◽  
pp. 1543
Author(s):  
Céline Hanzen ◽  
Martyn C. Lucas ◽  
Gordon O'Brien ◽  
Colleen T. Downs ◽  
Sandi Willows-Munro
Keyword(s):  
Dna Barcoding ◽  
Environmental Dna ◽  
Western Indian Ocean ◽  
Genetic Distances ◽  
Kwazulu Natal ◽  
Coi Barcoding ◽  
Ocean Region ◽  
Coi Sequences ◽  
Freshwater Eels ◽  
Identification Tool

Freshwater eels (Anguilla spp.) have a long and complex catadromous life cycle. This unique feature, coupled with difficulty in separating species based on morphology, makes them complex targets for conservation. In this study we evaluated the utility of DNA barcoding using cytochrome oxidase I (COI) to delimit the four species of African eels found in the western Indian Ocean region. We collected 76 individual fin clips from the four eel species (A. mossambica, n=51; A. marmorata, n=17; A. bengalensis, n=6; A. bicolor, n=2) in the rivers of KwaZulu–Natal, South Africa, in 2016–18. Phylogenetic analysis of the COI sequences recovered all four species as monophyletic. Barcoding gap analyses were performed and there was no found overlap in inter- and intraspecific genetic distances. Consequently, the use of COI barcoding as an identification tool was found to be reliable for identifying African eels to the species level, which suggests that this marker should be included in future environmental DNA or metabarcoding studies.

scholarly journals Potential of DNA Barcoding for Detecting Quarantine Fungi

2013 ◽  
Vol 103 (11) ◽  
pp. 1103-1107 ◽  
Author(s):  
Ruifang Gao ◽  
Guiming Zhang
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Internal Transcribed Spacer ◽  
Pathogenic Fungi ◽  
International Standards ◽  
Detection Methods ◽  
Dna Barcodes ◽  
Interstate Disputes ◽  
The Status ◽  
Identification Tool

The detection of live quarantine pathogenic fungi plays an important role in guaranteeing regional biological safety. DNA barcoding, an emerging species identification technology, holds promise for the reliable, quick, and accurate detection of quarantine fungi. International standards for phytosanitary guidelines are urgently needed. The varieties of quarantine fungi listed for seven countries/regions, the currently applied detection methods, and the status of DNA barcoding for detecting quarantine fungi are summarized in this study. Two approaches have been proposed to apply DNA barcoding to fungal quarantine procedures: (i) to verify the reliability of known internal transcribed spacer (ITS)/cytochrome c oxidase subunit I (COI) data for use as barcodes, and (ii) to determine other barcodes for species that cannot be identified by ITS/COI. As a unique, standardizable, and universal species identification tool, DNA barcoding offers great potential for integrating detection methods used in various countries/regions and establishing international detection standards based on accepted DNA barcodes. Through international collaboration, interstate disputes can be eased and many problems related to routine quarantine detection methods can be solved for global trade.

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