scholarly journals Use of laser capture microdissection for the assessment of equine lamellar basal epithelial cell signalling in the early stages of laminitis

2014 ◽  
Vol 47 (4) ◽  
pp. 478-488 ◽  
Author(s):  
B. S. Leise ◽  
M. R. Watts ◽  
S. Roy ◽  
A. S. Yilmaz ◽  
H. Alder ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Laser Capture Microdissection ◽  
Cell Signalling ◽  
Early Stages ◽  
Basal Epithelial Cell ◽  
Laser Capture

Isolating Human Notochord Using Laser Capture Microdissection

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Youssef Yakkioui ◽  
Remco Santegoeds ◽  
Koo van Overbeeke ◽  
Andreas Herrler ◽  
Yasin Temel
Keyword(s):  
Laser Capture Microdissection ◽  
Laser Capture

scholarly journals Stromal MED12 exon 2 mutations in complex fibroadenomas of the breast

2020 ◽  
pp. jclinpath-2020-207062
Author(s):  
Edaise M da Silva ◽  
Francisco Beca ◽  
Ana Paula Martins Sebastiao ◽  
Melissa P Murray ◽  
Catarina Silveira ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Laser Capture Microdissection ◽  
Sanger Sequencing ◽  
Mediator Complex ◽  
Telomerase Reverse Transcriptase ◽  
Exon 2 ◽  
Stromal Component ◽  
Hotspot Mutations ◽  
Laser Capture

AimsHere we explore the presence of mediator complex subunit 12 (MED12) exon 2 and telomerase reverse transcriptase (TERT) promoter hotspot mutations in complex fibroadenomas (CFAs) of the breast.MethodsThe stromal components from 18 CFAs were subjected to Sanger sequencing of MED12 exon 2 and the TERT promoter hotspot loci. The epithelial and stromal components of two MED12 mutated CFAs were subjected to laser capture microdissection, and Sanger sequencing of MED12 exon 2, TERT promoter and PIK3CA exons 9 and 20, separately.ResultsMED12 exon 2 mutations were identified in the stroma of 17% of CFAs. The analyses of epithelial and stromal components, microdissected separately, revealed that MED12 mutations were restricted to the stroma. No TERT promoter or PIK3CA mutations in exons 9 and 20 were detected in analysed CFAs.ConclusionsLike conventional fibroadenomas, MED12 exon 2 mutations appear to be restricted to the stromal component of CFAs, supporting the notion that CFAs are stromal neoplasms.

scholarly journals Laser capture microdissection for transcriptomic profiles in human skin biopsies

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Silvia Santoro ◽  
Ignazio Diego Lopez ◽  
Raffaella Lombardi ◽  
Andrea Zauli ◽  
Ana Maria Osiceanu ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Capture Microdissection ◽  
Skin Biopsies ◽  
Laser Capture
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