Topical treatment with a two-component gel releasing nitric oxide cures C57BL/6 mice from cutaneous leishmaniasis caused byLeishmania major

ABSTRACT Current treatment of cutaneous leishmaniasis (CL) relies mainly on pentavalent antimonials salts and second-line drugs include pentamidine and amphotericin B, but these therapies have side effects and require parenteral administration. The aim of this work was to evaluate the topical formulations containing pentamidine isethionate (PI) in the experimental treatment of cutaneous leishmaniasis (CL). Golden hamsters (Mesocricetus auratus) were infected in the nose with Leishmania (Leishmania) amazonensis. Six treatment groups received different topical treatments of anhydrous or hydrating emulsions, for a maximum of 10 days, with an application of 50 mg day-1. After treatment tissue samples of lesions were evaluated by histology, transmission electron microscopy and biopsy cultivation. Compared with untreated group, topical treatment with hydrating emulsion with 10% PI and usnic acid (ACE5AU) showed significantly decrease in volume lesion (P= 0.028) on 20th day after the end of the treatment with reduction of 27.37%. Topical treatment with anhydrous emulsion with 10% PI and usnic acid (ACPU) reduces parasite burden in Golden hamsters. This study demonstrated the potential of topical treatment to reduce the number of parasites that could be combined with others drugs and to have a faster and more effective treatment of cutaneous leishmaniasis.

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Surfactant free synthesis of cationic nano-vesicles: A safe triple drug loaded vehicle for the topical treatment of cutaneous leishmaniasis

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2021.102490 ◽

2021 ◽

pp. 102490

Author(s):

Sidra Khalid ◽

Saad Salman ◽

Kashif Iqbal ◽

Fiza ur Rehman ◽

Iffat Ullah ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Topical Treatment

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A Controlled, Randomized-Blinded Clinical Trial to Assess the Efficacy of a Nitric Oxide Releasing Patch in the Treatment of Cutaneous Leishmaniasis by Leishmania (V.) panamensis

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2010.09-0287 ◽

2010 ◽

Vol 83 (1) ◽

pp. 97-101 ◽

Cited By ~ 32

Author(s):

Patricio López-Jaramillo ◽

Melvin Y. Rincón ◽

Ronald G. García ◽

Iván D. Vélez ◽

Daniel J. Smith ◽

...

Keyword(s):

Nitric Oxide ◽

Clinical Trial ◽

Cutaneous Leishmaniasis ◽

Nitric Oxide Releasing

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Expression of Inducible Nitric Oxide Synthase in Skin Lesions of Patients with American Cutaneous Leishmaniasis

Infection and Immunity ◽

10.1128/iai.70.8.4638-4642.2002 ◽

2002 ◽

Vol 70 (8) ◽

pp. 4638-4642 ◽

Cited By ~ 53

Author(s):

Muna Qadoumi ◽

Inge Becker ◽

Norbert Donhauser ◽

Martin Röllinghoff ◽

Christian Bogdan

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Cutaneous Leishmaniasis ◽

Leishmania Donovani ◽

Leishmania Major ◽

Parasite Burden ◽

Skin Lesions ◽

And Function ◽

Oxide Synthase

ABSTRACT Cytokine-inducible (or type 2) nitric oxide synthase (iNOS) is indispensable for the resolution of Leishmania major or Leishmania donovani infections in mice. In contrast, little is known about the expression and function of iNOS in human leishmaniasis. Here, we show by immunohistological analysis of skin biopsies from Mexican patients with local (LCL) or diffuse (DCL) cutaneous leishmaniasis that the expression of iNOS was most prominent in LCL lesions with small numbers of parasites whereas lesions with a high parasite burden (LCL or DCL) contained considerably fewer iNOS-positive cells. This is the first study to suggest an antileishmanial function of iNOS in human Leishmania infections in vivo.

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