Pregnancy enhances the innate immune response in experimental cutaneous leishmaniasis through hormone-modulated nitric oxide production

2008 ◽  
Vol 83 (6) ◽  
pp. 1413-1422 ◽  
Author(s):  
Y. Osorio ◽  
D. L. Bonilla ◽  
A. G. Peniche ◽  
P. C. Melby ◽  
B. L. Travi
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Cutaneous Leishmaniasis ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Myeloid-Related Proteins Rapidly Modulate Macrophage Nitric Oxide Production during Innate Immune Response

2008 ◽  
Vol 181 (5) ◽  
pp. 3595-3601 ◽  
Author(s):  
Philippe Pouliot ◽  
Isabelle Plante ◽  
Marie-Astrid Raquil ◽  
Philippe A. Tessier ◽  
Martin Olivier
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Related Proteins

scholarly journals Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase.

1995 ◽  
Vol 181 (1) ◽  
pp. 63-70 ◽  
Author(s):  
N K Worrall ◽  
W D Lazenby ◽  
T P Misko ◽  
T S Lin ◽  
C P Rodi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Biologically Active ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The role of nitric oxide in the immune response to allogeneic tissue was explored in an in vivo cardiac transplant model in the rat. Nitric oxide production during organ rejection was demonstrated by elevations in systemic serum nitrite/nitrate levels and by electron paramagnetic resonance spectroscopy. Messenger RNA for the inducible nitric oxide synthase enzyme was detected in the rejecting allografted heart, but not in the nonrejecting isografted heart. The enzyme was demonstrated to be biologically active by the in vitro conversion of L-arginine to L-citrulline and was immunohistochemically localized to the infiltrating inflammatory cells. Treatment with aminoguanidine, a preferential inhibitor of the inducible nitric oxide synthase isoform, prevented the increased nitric oxide production in the transplanted organ and significantly attenuated the pathogenesis of acute rejection. Aminoguanidine treatment prolonged graft survival, improved graft contractile function, and significantly reduced the histologic grade of rejection. These results suggest an important role for nitric oxide in mediating the immune response to allogeneic tissue. Inhibition of inducible nitric oxide synthase may provide a novel therapeutic modality in the management of acute transplant rejection and of other immune-mediated processes.

scholarly journals miR-155 modulates microglia-mediated immune response by down-regulating SOCS-1 and promoting cytokine and nitric oxide production

Immunology ◽  
2011 ◽  
Vol 135 (1) ◽  
pp. 73-88 ◽  
Author(s):  
Ana L. Cardoso ◽  
Joana R. Guedes ◽  
Luís Pereira de Almeida ◽  
Maria C. Pedroso de Lima
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Rifampin Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial Cells

2006 ◽  
Vol 50 (1) ◽  
pp. 396-398 ◽  
Author(s):  
Yael Yuhas ◽  
Eva Berent ◽  
Hila Ovadiah ◽  
Inbar Azoulay ◽  
Shai Ashkenazi
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Alveolar Epithelial Cells ◽  
Nitric Oxide Production ◽  
Alveolar Cells ◽  
Alveolar Epithelial ◽  
Oxide Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide ◽  
Human Alveolar Epithelial Cells

ABSTRACT Rifampin increased nitric oxide production and inducible nitric oxide synthase expression in alveolar cells stimulated with cytokines. Nitric oxide concentrations after induction with cytokines, cytokines with 10 μg/ml rifampin, and cytokines with 50 μg/ml rifampin were 3.2, 4.5, and 8.8 μM, respectively (P < 0.02 versus cytokines alone). This indicates that rifampin modulates the immune response.

scholarly journals Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Satoshi Gondaira ◽  
Koji Nishi ◽  
Jumpei Fujiki ◽  
Hidetomo Iwano ◽  
Reina Watanabe ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Innate Immune Response ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Functional Expression ◽  
Innate Immune ◽  
Cytokine Gene Expression ◽  
Mycoplasma Bovis ◽  
Bovine Neutrophils

AbstractMycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs.

scholarly journals Exposure to artificial light at night alters innate immune response in wild great tit nestlings

2021 ◽  
Author(s):  
Ziegler Ann-Kathrin ◽  
Watson Hannah ◽  
Hegemann Arne ◽  
Meitern Richard ◽  
Canoine Virginie ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Immune Function ◽  
Innate Immune Response ◽  
Great Tit ◽  
Innate Immune ◽  
Artificial Light ◽  
Immune Challenge ◽  
Light At Night ◽  
Dark Night

The large-scale impact of urbanization on wildlife is rather well documented, however the mechanisms underlying the effects of urban environments on animal physiology and behaviour are still poorly understood. Here, we focused on one major urban pollutant - artificial light at night (ALAN) - and its effects on the capacity to mount an innate immune response in wild great tit Parus major nestlings. Exposure to ALAN alters circadian rhythms of physiological processes, by disrupting the nocturnal production of the hormone melatonin. Nestlings were exposed to a light source emitting 3 lux for seven consecutive nights. Subsequently, nestlings were immune-challenged with a lipopolysaccharide injection, and we measured haptoglobin and nitric oxide levels pre- and post-injection. Both haptoglobin and nitric oxide are important markers for innate immune function. We found that ALAN exposure altered the innate immune response, with ALAN nestlings having lower haptoglobin and higher nitric oxide levels after the immune-challenge compared to dark-night nestlings. Unexpectedly, nitric oxide levels were overall, lower after the immune-challenge than before. These effects were likely mediated by melatonin, since ALAN-treated birds had on average 49% lower melatonin levels than the dark-night birds. ALAN exposure did not have any clear effects on nestling growth. This study provides a potential physiological mechanism underlying the documented differences in immune function between urban and rural birds observed in other studies. Moreover, it gives evidence that ALAN exposure affects nestling physiology, potentially causing long-term effects on physiology and behaviour, which ultimately can affect their fitness.

scholarly journals Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Laura Enedina Soto-Serna ◽  
Mariana Diupotex ◽  
Jaime Zamora-Chimal ◽  
Adriana Ruiz-Remigio ◽  
José Delgado-Domínguez ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
T Cell Activation ◽  
Immune Modulation ◽  
Nitric Oxide Production ◽  
Class I Mhc ◽  
Mhc I ◽  
Oxide Production ◽  
Parasite Survival ◽  
Antigen Presenting

Exosomes are extracellular microvesicles of endosomal origin (multivesicular bodies, MVBs) constitutively released by eukaryotic cells by fusion of MVBs to the plasma membrane. The exosomes from Leishmania parasites contain an array of parasite molecules such as virulence factors and survival messengers, capable of modulating the host immune response and thereby favoring the infection of the host. We here show that exosomes of L. mexicana amastigotes (aExo) contain the virulence proteins gp63 and PP2C. The incubation of aExo with bone marrow-derived macrophages (BMMs) infected with L. mexicana led to their internalization and were found to colocalize with the cellular tetraspanin CD63. Furthermore, aExo inhibited nitric oxide production of infected BMMs, permitting enhanced intracellular parasite survival. Expressions of antigen-presenting (major histocompatibility complex class I, MHC-I, and CD1d) and costimulatory (CD86 and PD-L1) molecules were modulated in a dose-dependent fashion. Whereas MHC-I, CD86 and PD-L1 expressions were diminished by exosomes, CD1d was enhanced. We conclude that aExo of L. mexicana are capable of decreasing microbicidal mechanisms of infected macrophages by inhibiting nitric oxide production, thereby enabling parasite survival. They also hamper the cellular immune response by diminishing MHC-I and CD86 on an important antigen-presenting cell, which potentially interferes with CD8 T cell activation. The enhanced CD1d expression in combination with reduction of PD-L1 on BMMs point to a potential shift of the activation route towards lipid presentations, yet the effectivity of this immune activation is not evident, since in the absence of costimulatory molecules, cellular anergy and tolerance would be expected.

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