scholarly journals Non‐neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5‐positive stem cells in mice

FEBS Journal ◽  
2014 ◽  
Vol 281 (20) ◽  
pp. 4672-4690 ◽  
Author(s):  
Toshio Takahashi ◽  
Hiroe Ohnishi ◽  
Yuki Sugiura ◽  
Kurara Honda ◽  
Makoto Suematsu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endogenous Regulator ◽  
Proliferation And Differentiation

Application of Nanomaterials in Regulating the Fate of Adipose-derived Stem Cells

2021 ◽  
Vol 16 (1) ◽  
pp. 3-13
Author(s):  
Lang Wang ◽  
Yong Li ◽  
Maorui Zhang ◽  
Kui Huang ◽  
Shuanglin Peng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Research ◽  
Adult Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Proliferation And Differentiation ◽  
Recent Developments ◽  
Good Biocompatibility ◽  
Regulatory Effects ◽  
New Treatment

Adipose-derived stem cells are adult stem cells which are easy to obtain and multi-potent. Stem-cell therapy has become a promising new treatment for many diseases, and plays an increasingly important role in the field of tissue repair, regeneration and reconstruction. The physicochemical properties of the extracellular microenvironment contribute to the regulation of the fate of stem cells. Nanomaterials have stable particle size, large specific surface area and good biocompatibility, which has led them being recognized as having broad application prospects in the field of biomedicine. In this paper, we review recent developments of nanomaterials in adipose-derived stem cell research. Taken together, the current literature indicates that nanomaterials can regulate the proliferation and differentiation of adipose-derived stem cells. However, the properties and regulatory effects of nanomaterials can vary widely depending on their composition. This review aims to provide a comprehensive guide for future stem-cell research on the use of nanomaterials.

In Vitro Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Regenerating Template

2015 ◽  
Vol 05 (999) ◽  
pp. 1-1
Author(s):  
Abu Bakar Mohd Hilmi ◽  
Mohd Noor Norhayati ◽  
Ahmad Sukari Halim ◽  
Chin Keong Lim ◽  
Zulkifli Mustafa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Human Hair ◽  
Human Hair Follicle ◽  
In Vitro Proliferation ◽  
Proliferation And Differentiation ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yaozong Wang ◽  
Naikun Sun ◽  
Yinlong Zhang ◽  
Bin Zhao ◽  
Zheyi Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Composite Scaffold ◽  
Adipose Derived Stem Cells ◽  
Proliferation And Differentiation

scholarly journals Fluid Flow Mechanical Stimulation-Assisted Cartridge Device for the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 927
Author(s):  
Ki-Taek Lim ◽  
Dinesh-K. Patel ◽  
Sayan-Deb Dutta ◽  
Keya Ganguly
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Flow ◽  
Osteogenic Differentiation ◽  
Flow Condition ◽  
Cultured Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Static Conditions

Human mesenchymal stem cells (hMSCs) have the potential to differentiate into different types of mesodermal tissues. In vitro proliferation and differentiation of hMSCs are necessary for bone regeneration in tissue engineering. The present study aimed to design and develop a fluid flow mechanically-assisted cartridge device to enhance the osteogenic differentiation of hMSCs. We used the fluorescence-activated cell-sorting method to analyze the multipotent properties of hMSCs and found that the cultured cells retained their stemness potential. We also evaluated the cell viabilities of the cultured cells via water-soluble tetrazolium salt 1 (WST-1) assay under different rates of flow (0.035, 0.21, and 0.35 mL/min) and static conditions and found that the cell growth rate was approximately 12% higher in the 0.035 mL/min flow condition than the other conditions. Moreover, the cultured cells were healthy and adhered properly to the culture substrate. Enhanced mineralization and alkaline phosphatase activity were also observed under different perfusion conditions compared to the static conditions, indicating that the applied conditions play important roles in the proliferation and differentiation of hMSCs. Furthermore, we determined the expression levels of osteogenesis-related genes, including the runt-related protein 2 (Runx2), collagen type I (Col1), osteopontin (OPN), and osteocalcin (OCN), under various perfusion vis-à-vis static conditions and found that they were significantly affected by the applied conditions. Furthermore, the fluorescence intensities of OCN and OPN osteogenic gene markers were found to be enhanced in the 0.035 mL/min flow condition compared to the control, indicating that it was a suitable condition for osteogenic differentiation. Taken together, the findings of this study reveal that the developed cartridge device promotes the proliferation and differentiation of hMSCs and can potentially be used in the field of tissue engineering.

Decellularized matrix could affect the proliferation and differentiation of periodontal ligament stem cells in vitro

2021 ◽  
Author(s):  
Jia‐Ping Huang ◽  
Yan‐Min Wu ◽  
Jia‐Mei Liu ◽  
Lan Zhang ◽  
Bo‐Xiu Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cells ◽  
Proliferation And Differentiation

scholarly journals Human adipose-derived stem cells cultured in keratinocyte serum free medium: Donor’s age does not affect the proliferation and differentiation capacities

2013 ◽  
Vol 20 (1) ◽  
pp. 59 ◽  
Author(s):  
Dah-Ching Ding ◽  
Hsiang-Lan Chou ◽  
Wei-Ting Hung ◽  
Hwan-Wun Liu ◽  
Tang-Yuan Chu
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Proliferation And Differentiation ◽  
Cells Cultured

scholarly journals Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane

2018 ◽  
Vol 53 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Fernanda Gimenez de Souza ◽  
Beatriz Luci Fernandes ◽  
Carmen Lucia Kuniyoshi Rebelatto ◽  
Alessandra Melo de Aguiar ◽  
Letícia Fracaro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Plasma Membrane ◽  
Proliferation And Differentiation
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