An unusual ZZ/ZW sex chromosome system inCharacidiumfishes (Crenuchidae, Characiformes) with the presence of rDNA sites

2009 ◽  
Vol 75 (2) ◽  
pp. 448-453 ◽  
Author(s):  
R. B. Noleto ◽  
A. P. Amorim ◽  
M. R. Vicari ◽  
R. F. Artoni ◽  
M. M. Cestari
Keyword(s):  
Sex Chromosome ◽  
Rdna Sites ◽  
Sex Chromosome System

scholarly journals Highly Rearranged Karyotypes and Multiple Sex Chromosome Systems in Armored Catfishes from the Genus Harttia (Teleostei, Siluriformes)

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1366
Author(s):  
Geize Aparecida Deon ◽  
Larissa Glugoski ◽  
Marcelo Ricardo Vicari ◽  
Viviane Nogaroto ◽  
Francisco de Menezes Cavalcante Sassi ◽  
...  
Keyword(s):  
Sex Chromosome ◽  
Neotropical Region ◽  
Valid Species ◽  
Comparative Genomic ◽  
Isolated Populations ◽  
Shared Content ◽  
Rdna Sites ◽  
Number Variation ◽  
Fish Group ◽  
Sex Chromosome System

Harttia comprises an armored catfish genus endemic to the Neotropical region, including 27 valid species with low dispersion rates that are restricted to small distribution areas. Cytogenetics data point to a wide chromosomal diversity in this genus due to changes that occurred in isolated populations, with chromosomal fusions and fissions explaining the 2n number variation. In addition, different multiple sex chromosome systems and rDNA loci location are also found in some species. However, several Harttia species and populations remain to be investigated. In this study, Harttia intermontana and two still undescribed species, morphologically identified as Harttia sp. 1 and Harttia sp. 2, were cytogenetically analyzed. Harttia intermontana has 2n = 52 and 2n = 53 chromosomes, while Harttia sp. 1 has 2n = 56 and 2n = 57 chromosomes in females and males, respectively, thus highlighting the occurrence of an XX/XY1Y2 multiple sex chromosome system in both species. Harttia sp. 2 presents 2n = 62 chromosomes for both females and males, with fission events explaining its karyotype diversification. Chromosomal locations of the rDNA sites were also quite different among species, reinforcing that extensive rearrangements had occurred in their karyotype evolution. Comparative genomic hybridization (CGH) experiments among some Harttia species evidenced a shared content of the XY1Y2 sex chromosomes in three of them, thus pointing towards their common origin. Therefore, the comparative analysis among all Harttia species cytogenetically studied thus far allowed us to provide an evolutionary scenario related to the speciation process of this fish group.

Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes)

2017 ◽  
Vol 153 (2) ◽  
pp. 96-104 ◽  
Author(s):  
Patrik F. Viana ◽  
Tariq Ezaz ◽  
Leandro Marajó ◽  
Milena Ferreira ◽  
Jansen Zuanon ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Sex Chromosome ◽  
First Record ◽  
Nucleolus Organizer ◽  
X Chromosomes ◽  
Nucleolus Organizer Regions ◽  
Rdna Sites ◽  
Puffer Fish ◽  
Specific Amplification ◽  
Sex Chromosome System

The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family.

scholarly journals Cytogenetic characterization of Hypostomus soniae Hollanda-Carvalho & Weber, 2004 from the Teles Pires River, southern Amazon basin: evidence of an early stage of an XX/XY sex chromosome system

2019 ◽  
Vol 13 (4) ◽  
pp. 411-422 ◽  
Author(s):  
Luciene Castuera de Oliveira ◽  
Marcos Otávio Ribeiro ◽  
Gerlane de Medeiros Costa ◽  
Cláudio Henrique Zawadzki ◽  
Ana Camila Prizon-Nakajima ◽  
...  
Keyword(s):  
Amazon Basin ◽  
Sex Chromosome ◽  
Early Stage ◽  
Organizer Region ◽  
Nucleolus Organizer Region ◽  
Structural Difference ◽  
Chromosomal Polymorphism ◽  
Nucleolus Organizer ◽  
Rdna Sites ◽  
Sex Chromosome System

In the present study, we analyzed individuals of Hypostomus soniae (Loricariidae) collected from the Teles Pires River, southern Amazon basin, Brazil. Hypostomus soniae has a diploid chromosome number of 2n = 64 and a karyotype composed of 12 metacentric (m), 22 submetacentric (sm), 14 subtelocentric (st), and 16 acrocentric (a) chromosomes, with a structural difference between the chromosomes of the two sexes: the presence of a block of heterochromatin in sm pair No. 26, which appears to represent a putative initial stage of the differentiation of an XX/XY sex chromosome system. This chromosome, which had a heterochromatin block, and was designated proto-Y (pY), varied in the length of the long arm (q) in comparison with its homolog, resulting from the addition of constitutive heterochromatin. It is further distinguished by the presence of major ribosomal cistrons in a subterminal position of the long arm (q). The Nucleolus Organizer Region (NOR) had different phenotypes among the H. soniae individuals in terms of the number of Ag-NORs and 18S rDNA sites. The origin, distribution and maintenance of the chromosomal polymorphism found in H. soniae reinforced the hypothesis of the existence of a proto-Y chromosome, demonstrating the rise of an XX/XY sex chromosome system.

scholarly journals Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

2019 ◽  
Vol 20 (12) ◽  
pp. 2944 ◽  
Author(s):  
Sassi ◽  
Oliveira ◽  
Bertollo ◽  
Nirchio ◽  
Hatanaka ◽  
...  
Keyword(s):  
Sex Chromosome ◽  
Distribution Patterns ◽  
Chromosomal Evolution ◽  
Evolutionary Relationships ◽  
Comparative Genomic ◽  
Banding Patterns ◽  
Rdna Sites ◽  
Sex Chromosome System ◽  
High Level ◽  
Chromosomal Sequences

We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA3+ banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA3+) regions, while L. melanoguttata shows evidence of a clear intercalated CMA3+ banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA3+, and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families.

scholarly journals Evolution of a Multiple Sex-Chromosome System by Three-Sequential Translocations among Potential Sex-Chromosomes in the Taiwanese Frog Odorrana swinhoana

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 661
Author(s):  
Ikuo Miura ◽  
Foyez Shams ◽  
Si-Min Lin ◽  
Marcelo de Bello Cioffi ◽  
Thomas Liehr ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Rare Case ◽  
Sex Chromosome ◽  
Sex Chromosome System ◽  
Brown Frog ◽  
Male Specific

Translocation between sex-chromosomes and autosomes generates multiple sex-chromosome systems. It happens unexpectedly, and therefore, the evolutionary meaning is not clear. The current study shows a multiple sex chromosome system comprising three different chromosome pairs in a Taiwanese brown frog (Odorrana swinhoana). The male-specific three translocations created a system of six sex-chromosomes, ♂X1Y1X2Y2X3Y3 -♀X1X1X2X2X3X3. It is unique in that the translocations occurred among three out of the six members of potential sex-determining chromosomes, which are known to be involved in sex-chromosome turnover in frogs, and the two out of three include orthologs of the sex-determining genes in mammals, birds and fishes. This rare case suggests sex-specific, nonrandom translocations and thus provides a new viewpoint for the evolutionary meaning of the multiple sex chromosome system.

scholarly journals Karyotype description of three species of Loricariidae (Siluriformes) and occurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003

2006 ◽  
Vol 4 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Renildo Ribeiro de Oliveira ◽  
Issakar Lima Souza ◽  
Paulo Cesar Venere
Keyword(s):  
Chromosome Pair ◽  
Sex Chromosome ◽  
Sexual System ◽  
Freshwater Systems ◽  
Phylogenetic Studies ◽  
Cytogenetic Data ◽  
Sex Chromosome System

The neotropical freshwater systems have a high number of catfish species (Siluriformes), and many of those are denominated "cascudos" in Brazil. Cytogenetic data about three "cascudos" species fished in the rio Araguaia are described in the present study. The Pterygoplichthys joselimaianus showed 2n=52, with 28 metacentrics (M) chromosomes, 16 submetacentrics (SM) and 8 subtelocentrics/acrocentrics (ST/A) in both sexes. Hemiancistrus spinosissimus showed 2n=52, with karyotype formulae 26M+22SM+4ST, in both sexes. Hemiancistrusspilomma also showed 2n=52, but in this species a ZZ/ZW sex chromosome system (25M+21SM+6ST in females and 24M+22SM+6ST in males) was observed. The cells from H. spinosissimus and P. joselimaianus showed one chromosome pair bearing Ag-NORs, while in the H. spilomma three chromosome pairs bearing Ag-NORs were detected. The data showed in this work reveal particular chromosomal characteristics, important for a good recognition of both Hemincistrus species, and also show the importance of the insertion of cytogenetic data on taxonomic phylogenetic studies.

scholarly journals Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region

2012 ◽  
Vol 35 (4) ◽  
pp. 770-776 ◽  
Author(s):  
Elisangela Bellafronte ◽  
Michelle Orane Schemberger ◽  
Roberto Ferreira Artoni ◽  
Orlando Moreira Filho ◽  
Marcelo Ricardo Vicari
Keyword(s):  
Chromosomal Region ◽  
Sex Chromosome ◽  
Sex Chromosome System

scholarly journals A new species of the genus Rhaphidosoma Amyot et Serville, 1843 (Heteroptera, Reduviidae), with data on its chromosome complement

2021 ◽  
Vol 15 (4) ◽  
pp. 467-505
Author(s):  
Dmitry A. Gapon ◽  
Valentina G. Kuznetsova ◽  
Anna Maryańska-Nadachowska
Keyword(s):  
New Species ◽  
Fore Wing ◽  
Sex Chromosome ◽  
Complex Structure ◽  
Chromosome Complement ◽  
Morphological Description ◽  
Oriental Region ◽  
Western Asia ◽  
Sex Chromosome System ◽  
A New Species

A new species, Rhaphidosoma paganicumsp. nov. (Heteroptera: Reduviidae: Harpactorinae: Rhaphidosomatini), is described from the Dry Zone of Myanmar. It is the fifth species of Rhaphidosoma Amyot et Serville, 1843, known from the Oriental Region, and the first record of the genus for Myanmar and Indochina. The structure of the external and internal terminalia of the male and female is described and illustrated in detail. The completely inflated endosoma is described for the first time in reduviids. The complex structure of the ductus seminis is shown; it terminates with a voluminous seminal chamber which opens with a wide secondary gonopore and may be a place where spermatophores are formed. The new species is compared with all congeners from the Oriental Region and Western Asia. It is characterised by the absence of distinct tubercles on the abdominal tergites of the male, the presence only two long tubercles and small rounded ones on the abdominal tergites VII and VI, respectively, in the female, the presence of short fore wing vestiges which are completely hidden under longer fore wing vestiges, and other characters. In addition to the morphological description, an account is given of the male karyotype and the structure of testes of Rh. paganicumsp. nov. and another species of Harpactorinae, Polididus armatissimus Stål, 1859 (tribe Harpactorini). It was found that Rh. paganicumsp. nov. has a karyotype comprising 12 pairs of autosomes and a multiple sex chromosome system (2n♂=24A+X1X2X3Y), whereas P. armatissimus has a karyotype comprising five pairs of autosomes and a simple sex chromosome system (2n♂=10A+XY). The males of these species were found to have seven and nine follicles per testis, respectively. FISH mapping of 18S ribosomal DNA (major rDNA) revealed hybridisation signals on two of the four sex chromosomes (Y and one of the Xs) in Rh. paganicumsp. nov. and on the largest pair of autosomes in P. armatissimus. The presence of the canonical “insect” (TTAGG)n telomeric repeat was detected in the chromosomes of both species. This is the first application of FISH in the tribe Raphidosomatini and in the genus Polididus Stål, 1858.

Sign in / Sign up

Export Citation Format

Share Document