Rapid culture-free identification and molecular typing of Shiga toxin-producingEscherichia coliby PCR-RFLP

2008 ◽  
Vol 52 (6) ◽  
pp. 310-313 ◽  
Author(s):  
Kensuke Shima ◽  
Naohisa Kawamura ◽  
Atsushi Hinenoya ◽  
Norihiko Sugimoto ◽  
Yuluo Wu ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Molecular Typing ◽  
Pcr Rflp

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

2011 ◽  
Vol 14 (2) ◽  
pp. 285-286 ◽  
Author(s):  
J. Karakulska ◽  
A. Pobucewicz ◽  
P. Nawrotek ◽  
M. Muszyńska ◽  
A. Furowicz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Genetic Relationship ◽  
Molecular Typing ◽  
Rapd Analysis ◽  
Milk Samples ◽  
Rapd Pcr ◽  
Pcr Rflp ◽  
Mastitic Milk ◽  
Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

scholarly journals Use of the espZ Gene Encoded in the Locus of Enterocyte Effacement for Molecular Typing of Shiga Toxin-Producing Escherichia coli

2006 ◽  
Vol 44 (2) ◽  
pp. 449-458 ◽  
Author(s):  
M. W. Gilmour ◽  
D. M. Tracz ◽  
A. K. Andrysiak ◽  
C. G. Clark ◽  
S. Tyson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Typing ◽  
Enterocyte Effacement

Determination of flagellar types by PCR-RFLP analysis of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) strains isolated from animals in São Paulo, Brazil

2012 ◽  
Vol 92 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Claudia de Oliveira Ayala ◽  
Ana Carolina Ramos Moreno ◽  
Marina Baquerizo Martinez ◽  
Ylanna Kelner Burgos ◽  
Antonio Fernando Pestana de Castro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Rflp Analysis ◽  
Sao Paulo ◽  
São Paulo ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Pcr Rflp

First documented case of human infection with ovine Shiga-toxin-producingEscherichia coliserotype O52:H45

2014 ◽  
Vol 60 (6) ◽  
pp. 417-418 ◽  
Author(s):  
Sadjia Bekal ◽  
Danielle Ramsay ◽  
Fabien Rallu ◽  
Pierre Pilon ◽  
Matthew Gilmour ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Typing ◽  
Human Infection ◽  
Causal Agent ◽  
Child Exposure ◽  
Documented Case ◽  
Sheep Meat ◽  
Concurrent Case

We report a concurrent case of infection with non-O157 Shiga-toxin-producing Escherichia coli (STEC) strain in an 8-month-old child. Laboratory and epidemiological investigations indicated child exposure to contaminated sheep meat following the Muslim feast of sacrifice (Eid al-Adha). Microbiological and molecular typing confirmed that the ovine strain O52:H45 (stx1+, eae–, hlyA–) was the causal agent. This is the first documented case of human infection to this STEC serotype.

scholarly journals Molecular Typing of Brucella melitensis and B. abortus from Human Blood Samples Using PCR-RFLP Method

2013 ◽  
Vol 6 (6) ◽  
Author(s):  
Reza Mirnejad ◽  
Mozafar Mohammadi ◽  
Ali Majdi ◽  
Niloufar Taghizoghi ◽  
Vahhab Piranfar
Keyword(s):  
Human Blood ◽  
Molecular Typing ◽  
Brucella Melitensis ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Human Blood Samples

scholarly journals Ongoing haemolytic uraemic syndrome (HUS) outbreak caused by sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157, Germany, December 2016 to May 2017

2017 ◽  
Vol 22 (21) ◽  
Author(s):  
Sabine Vygen-Bonnet ◽  
Bettina Rosner ◽  
Hendrik Wilking ◽  
Angelika Fruth ◽  
Rita Prager ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Typing ◽  
Haemolytic Uraemic Syndrome ◽  
Case Control Study ◽  
Case Control ◽  
Geographically Dispersed ◽  
Minced Meat ◽  
Close Relatedness ◽  
Control Study

We report an ongoing, protracted and geographically dispersed outbreak of haemolytic uraemic syndrome (HUS) and gastroenteritis in Germany, involving 30 cases since December 2016. The outbreak was caused by the sorbitol-fermenting immotile variant of Shiga toxin-producing (STEC) Escherichia coli O157. Molecular typing revealed close relatedness between isolates from 14 cases. One HUS patient died. Results of a case–control study suggest packaged minced meat as the most likely food vehicle. Food safety investigations are ongoing.

scholarly journals Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

2008 ◽  
Vol 70 (9) ◽  
pp. 907-913 ◽  
Author(s):  
Yoshimi TERAYAMA ◽  
Hirotomo KATO ◽  
Eduardo A. GOMEZ ◽  
Hiroshi UEZATO ◽  
Manuel CALVOPIÑA ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Molecular Typing ◽  
Sand Fly ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene ◽  
Pcr Rflp

Molecular typing of Indian Mycoplasma gallisepticum isolates

2019 ◽  
Author(s):  
Susitha Rajkumar ◽  
Maddula Ramkoti Reddy ◽  
Ramesh Somvanshi
Keyword(s):  
Sequence Analysis ◽  
Molecular Typing ◽  
Sequence Type ◽  
Field Isolates ◽  
Economic Threat ◽  
Pcr Rflp ◽  
Indian Isolates ◽  
Unknown Gene ◽  
Clinical Conditions ◽  
First Time

M. gallisepticum infection is a serious economic threat to the poultry industry in India, and the genetic diversity of Indian isolates is unknown. Gene-targeted sequence analysis of M. gallisepticum was conducted for the first time to map the diversity among different Indian isolates from different regions and from different clinical conditions. Sequence analysis of the mgc2 gene grouped the 13 field isolates into four groups. The most prevalent sequence type, the group1 shared 100% identity with the foreign strains THA 3CK08 (Thailand) and UHP1CK99 (Israel). Groups two and three were novel, and were genetically distinct from the strains outside the country. Group four shared 100% similarity with the vaccine strain MG6/85. The nucleotide similarity between the 4 groups ranged from 94.3-99.2%. A PCR-RFLP method using HaeII was tested and validated for its ability to differentiate the Indian field isolates from vaccine strains.

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