Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)-A31+Gastric Cancer Patients byin vitroStimulation with Antigenic Peptide of Signet Ring Cell Carcinoma

34 Background: The aim of the study was measuring biochemical indices reflecting endotoxicosis syndrome in gastric cancer patients with adenocarcinoma of different grades and signet ring cell carcinoma. Methods: The study included 67 patients with gastric cancer divided into 4 groups depending on the tumor histotype: well- and moderately-differentiated adenocarcinoma (G1-2); poorly-differentiated adenocarcinoma (G3); signet ring cell carcinoma (SRCC); combined gastric tumors; and 12 healthy donors. The content of medium mass molecules (MMM254 and MMM280), total and effective albumin concentrations (TCA and ECA), toxicity index (IT = TCA/ECA-1) and intoxication factor (IF = MMM254/ECA⋅1000) were used to assess the level of endogenous intoxication. The Statistika 6.0 software package was used. Results: IT, reflecting the functional state of the albumin molecule, significantly exceeded the level in donors: by 2.3 times in G1-2, by 3.3 times in G3, by 3.2 times in SRCC (p < 0.01-0.001), by 2.2 times in mixed tumors (p < 0.05). The greatest increase in IT – by 4.6 times – was observed in patients with serosal invasion and tumor spread to the adjacent structures (T4 in the TNM classification). IF, which is an integral indicator of endotoxemia, in all groups of patients was significantly (p < 0.01-0.00001) higher than in donors. The increase was 55.2% in patients with G1-2, 132.7% for G3, 234.6% for T4, 66.8% for SRCC, and 59.6% for mixed tumors. The dynamic observation of patients showed that a sharp increase in the studied parameters was followed by relapses. Conclusions: The increase in IT and IF in all groups of patients indicated an enhancement of endotoxemia in gastric tumors, mainly due to the decreased detoxification capacity of albumin, as high levels of MMM were observed only in some patients. The changes were significantly more expressed in patients with G3, with the maximal changes in all the studied endotoxicosis parameters in T4. The results demonstrated the diagnostic significance of the detoxification ability of albumin for the evaluation of the state of gastric cancer patients and for the adequate treatment planning.

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Different clinicopathologic features and prognostic significance of signet ring cell histology in early and locally advanced gastric cancer patients

Clinics and Research in Hepatology and Gastroenterology ◽

10.1016/j.clinre.2020.05.006 ◽

2020 ◽

pp. 101454 ◽

Cited By ~ 2

Author(s):

Bochao Zhao ◽

Huiwen Lu ◽

Rui Luo ◽

Shiyang Bao ◽

Di Mei ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

Locally Advanced ◽

Prognostic Significance ◽

Signet Ring Cell ◽

Clinicopathologic Features ◽

Locally Advanced Gastric Cancer ◽

Signet Ring ◽

Ring Cell ◽

Gastric Cancer Patients

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Different prognostic significance of signet ring cell histology for early and advanced gastric cancer patients: a systematic review and meta-analysis

Expert Review of Gastroenterology & Hepatology ◽

10.1080/17474124.2020.1769476 ◽

2020 ◽

Vol 14 (6) ◽

pp. 499-509

Author(s):

Bochao Zhao ◽

Wu Lv ◽

Jingting Zhang ◽

Jiale Zhang ◽

Baojun Huang ◽

...

Keyword(s):

Gastric Cancer ◽

Systematic Review ◽

Advanced Gastric Cancer ◽

Cancer Patients ◽

Meta Analysis ◽

Prognostic Significance ◽

Signet Ring Cell ◽

Signet Ring ◽

Ring Cell ◽

Gastric Cancer Patients

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Signet ring cell percentage in poorly cohesive gastric cancer patients: A potential novel predictor of survival

European Journal of Surgical Oncology ◽

10.1016/j.ejso.2021.09.003 ◽

2021 ◽

Author(s):

Franco Roviello ◽

Luigi Marano ◽

Maria Raffaella Ambrosio ◽

Luca Resca ◽

Alessia D'Ignazio ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

Signet Ring Cell ◽

Cell Percentage ◽

Signet Ring ◽

Ring Cell ◽

Gastric Cancer Patients

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Preoperative Systemic Immune-Inflammation Index (SII) for Predicting the Survival of Patients with Stage I-III Gastric Cancer with a Signet-Ring Cell (SRC) Component

BioMed Research International ◽

10.1155/2020/5038217 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Ziyu Zhu ◽

Xiliang Cong ◽

Rui Li ◽

Xin Yin ◽

Chunfeng Li ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

Prognostic Value ◽

Curative Resection ◽

Signet Ring Cell ◽

Study Cohort ◽

Signet Ring ◽

Ring Cell ◽

Immune Inflammation ◽

Gastric Cancer Patients

Background. Recently, a novel systemic immune-inflammation index (SII) based on peripheral lymphocytes, neutrophils, and platelets has been reported to be correlated with patient prognosis in several malignancies, including gastric cancer. However, the prognostic value of the SII for gastric cancer patients with a signet-ring cell (SRC) component has not yet been reported. In this study, we aimed to assess the prognostic value of the SII in gastric cancer patients with an SRC component after curative resection. Methods. This study was a retrospective analysis of 512 GC patients with an SRC component who underwent curative resection. The prognostic value of the SII was analyzed by the Kaplan-Meier method and Cox proportional hazards regression model. Results. In our study cohort, an optimal cut-off value for the SII of 527 was used to stratify patients with gastric cancer (GC) into low (<527) and high SII (≥527) groups. Our study indicated that a high SII (≥527) was significantly correlated with a large tumor size (p<0.001), infiltration of serosa (p<0.001), lymph node metastasis (p<0.001), and advanced TNM stage (p<0.001). Univariate and multivariate analyses further demonstrated that a low SII was correlated with better clinical outcome and was an independent prognostic predictor in GC patients with an SRC component. Furthermore, the SII retained prognostic value in the subgroup analysis, including subgroup of different TNM stages and pure or mixed signet-ring cell carcinomas (SRCCs). Conclusion. The SII is a simple, promising, and practical prognostic biomarker for patients with surgically resected mixed SRCC and pure SRCC. The SII could complement current prognostic tools for better treatment planning and stratification of patients.

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Does signet-ring cell carcinoma of the stomach (SRCC) need stimulation of neoangiogenesis?

Journal of Clinical Oncology ◽

10.1200/jco.2018.36.4_suppl.33 ◽

2018 ◽

Vol 36 (4_suppl) ◽

pp. 33-33

Author(s):

Ekaterina Igorevna Surikova ◽

Elena Frantsiyants ◽

Irina A. Goroshinskaya ◽

Yulia A. Pogorelova ◽

Valeria A. Bandovkina ◽

...

Keyword(s):

Gastric Cancer ◽

Cell Carcinoma ◽

Signet Ring Cell Carcinoma ◽

Signet Ring Cell ◽

Tumor Tissues ◽

Signet Ring ◽

Resection Line ◽

Ring Cell ◽

Gastric Cancer Patients ◽

Tgf Β1

33 Background: Tumor neoangiogenesis is a complex coordinated process involving various regulatory molecules. Vascular endothelial growth factors, in particular VEGF-A, are important effectors. A multipotent TGF-β1 cytokine can modulate stromal angiogenesis reactions promoting the tumor growth. Our purpose was to study the function of the system of pro-angiogenic cytokines in tissues of stomach tumors of various histological types - adenocarcinoma (AC) and signet-ring cell carcinoma (SRCC). Methods: The concentrations of VEGF-A, VEGF-R1 and TGF-β1 were studied by ELISA in tumors, peritumoral zone and resection line tissues of gastric cancer patients without preoperative therapy: 15 patients with AC (T2-4N0-2M0, G2-G3, 50-84 years) and 10 patients with SRCC, comparable by sex, age and prevalence of cancer process. Results: Levels of VEGF-A, VEGF-R1 and TGF-β1 in the resection line tissues of AC and SRCC did not differ significantly. VEGF-A in AC tumor tissues exceeded significantly the levels in the resection line (by 2.3 times) and in the peritumoral zone (by 1.8 times). VEGF-A in SRCC tumor tissue did not differ significantly from the levels in the corresponding tissues of the resection line and peritumoral zone, but it was 2.6 times lower than in AC tumor tissues. VEGF-R1 levels in AC and SRCC were similar. TGF-β1 in AC tumor tissues was 3.2 and 2.6 times higher than in the resection line and peritumoral zone, respectively. TGF-β1 in SRCC tumor tissues did not differ from the levels in the peritumoral zone and in healthy tissues; TGF-β1 in tumor tissues was 3.2 times lower in SRCC than in AC. Conclusions: SRCC tumor tissues have significantly lower levels of pro-angiogenic VEGF-А and TGF-β1 cytokines, compared to AC tissues, which can indicate that SRCC has no need to form its own vasculature. It is probably associated with the biological characteristic of this histotype of gastric cancer - diffuse growth pattern, in contrast to the solid structure of AC.

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Comparison of 68Ga-FAPI-04 and 18F-FDG for the Detection of Primary Gastric Cancers and Metastasis

10.21203/rs.3.rs-326617/v1 ◽

2021 ◽

Author(s):

Donglang Jiang ◽

Xing Chen ◽

Zhiwen You ◽

Hao Wang ◽

Xiaoyun Zhang ◽

...

Keyword(s):

Gastric Cancer ◽

Cell Carcinoma ◽

Cancer Diagnosis ◽

Fdg Pet ◽

Standardized Uptake Value ◽

Signet Ring Cell Carcinoma ◽

Signet Ring Cell ◽

Gastric Cancers ◽

Signet Ring ◽

Ring Cell

Abstract Introduction Early and precise diagnosis and staging of gastric cancer are important for its treatment and management. However, the low sensitivity of 18F-fluorodeoxyglucose (18F-FDG) for gastric cancer diagnosis limits its application. Currently, the tracer 68Ga-FAPI, which targets fibroblast activation protein (FAP), is widely used to diagnose various cancers. However, the diagnostic value of 68Ga-FAPI in gastric cancer is still unclear. In this study, we aimed to investigate the potential advantage of 68Ga-FAPI-04 over 18F-FDG in the evaluation of gastric cancer.Methods: Thirty-eight patients with gastric cancer (31 with adenocarcinoma and 7 with signet ring cell carcinoma) were recruited for this study. All of the participants underwent 68Ga-FAPI-04 and 18F-FDG imaging by positron emission tomography (PET)/computed tomography (CT) or PET/magnetic resonance (MR). The results were interpreted by two experienced nuclear medicine physicians, and the maximum standardized uptake value (SUVmax) was calculated.Results: For the detection of primary gastric cancer, the sensitivities of 68Ga-FAPI-04 PET and 18F-FDG PET were 100% (38/38) and 81.6% (31/38), respectively. Four cases of adenocarcinoma and three cases of signet ring cell carcinoma were missed by 18F-FDG PET. The SUVmax of 68Ga-FAPI-04 in tumors greater than 4 cm (11.0 ± 4.5) was higher than tumors less than 4 cm (4.5 ± 3.2) (P = 0.0015). The SUVmax of 68Ga-FAPI-04 was higher in T2-4 tumors (9.7 ± 4.4) than in T1 tumors (3.1 ± 1.5) (P = 0.0002). For the detection of metastatic lesions, the sensitivities of 68Ga-FAPI-04 PET and 18F-FDG PET in 10 patients with regional lymph node metastasis and distant metastasis were 6/10 and 5/10, respectively.Conclusion: Compared to 18F-FDG PET, 68Ga-FAPI-04 PET had superior potential in detecting primary gastric cancers and metastatic lymph nodes, 68Ga-FAPI-04 PET also had a better performance on small gastric cancer detection. 68Ga-FAPI-04 PET could provide better performance for gastric cancer diagnosis and staging.

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