scholarly journals Dengue virus-induced human cytotoxic factor: production by peripheral blood leucocytes in vitro

2008 ◽  
Vol 102 (2) ◽  
pp. 262-267 ◽  
Author(s):  
R. MUKERJEE ◽  
U. C. CHATURVEDI ◽  
R. DHAWAN
Keyword(s):  
Dengue Virus ◽  
Peripheral Blood ◽  
Cytotoxic Factor ◽  
Factor Production ◽  
Peripheral Blood Leucocytes

In vitro effect of lentinan on the activation of immunological cells in Cyprinus carpio

2009 ◽  
Vol 6 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Zhang Liu ◽  
Cao Li-Ping ◽  
Ding Wei-Dong ◽  
Galina Jeney ◽  
Xu Pao ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Peripheral Blood ◽  
Head Kidney ◽  
Burst Activity ◽  
Griess Reaction ◽  
Nitroblue Tetrazolium Reduction ◽  
Diphenyl Tetrazolium Bromide ◽  
Vitro Effect ◽  
Peripheral Blood Leucocytes

AbstractMacrophages from the head kidney (HK) and peripheral blood leucocytes were isolated fromCyprinus carpio by Percoll gradient density centrifugation, cultured in vitro and exposed to different concentrations of the immunomodulator lentinan. To evaluate the immunostimulating effects of lentinan, proliferation of the peripheral blood leucocytes, respiratory burst of macrophages and interleukin-1β (IL-1β) gene mRNA expression of macrophages were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], NBT (nitroblue tetrazolium) reduction, Griess reaction and real-time polymerase chain reaction (PCR). Results showed that proliferation of peripheral blood leucocytes after 24 h incubation by the induction of lentinan at 100 and 1000 μg/ml was markedly stimulated. Lentinan at 1, 100 and 1000 μg/ml could significantly induce superoxide anions in macrophages. Production of nitric oxide by HK macrophages after 96 h incubation by lentinan showed that it had no conspicuous effect on nitrogen burst activity of macrophages. Moreover, it inhibited the nitrogen burst activity at higher doses. The expression of IL-1β in the HK macrophages after 24 h of polysaccharide stimulation showed that lentinan stimulated IL-1β expression in the head kidney in carp. Lentinan can modulate the immune response of C. carpio.

In Vitro Measurement of Phosphorylated Zidovudine in Peripheral Blood Leucocytes

1990 ◽  
Vol 12 (5) ◽  
pp. 481-489 ◽  
Author(s):  
Brian N. Stretcher ◽  
Amadeo J. Pesce ◽  
Joseph R. Wermeling ◽  
Paul E. Hurtubise
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Leucocytes

Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy

2015 ◽  
Vol 70 (1) ◽  
pp. 133-142 ◽  
Author(s):  
Aurélie Dupont ◽  
Marie-Claire De Pauw-Gillet ◽  
Joseph Schnitzler ◽  
Ursula Siebert ◽  
Krishna Das
Keyword(s):  
Electron Microscopy ◽  
Peripheral Blood ◽  
Harbour Seal ◽  
Peripheral Blood Leucocytes

scholarly journals Infectivity and viability of dengue virus infected hepatocytes cocultured with peripheral blood mononuclear cells from a healthy subject

2020 ◽  
Vol 29 (3) ◽  
pp. 260-7
Author(s):  
Sekar Asri Tresnaningtyas ◽  
Fithriyah Sjatha ◽  
Beti Ernawati Dewi
Keyword(s):  
Dengue Virus ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Blue Staining ◽  
Immunoperoxidase Staining ◽  
Peripheral Blood Mononuclear ◽  
Virus Serotype ◽  
Blood Mononuclear Cells

BACKGROUND Dengue virus (DENV) can infect and replicate in monocytes, resulting in antibody-dependent enhancement. The liver is the main target of DENV, and the infection mechanisms of DENV include direct cytopathic effects (CPEs) of the virus, mitochondrial dysfunction, and effect of cellular and humoral immune factors in the liver. This study was aimed to explore the infectivity of DENV and viability of human hepatocytes using Huh 7it-1 cells cocultured with peripheral blood mononuclear cells (PBMCs). METHODS Huh 7it-1 cells were infected with dengue virus serotype-2 (DENV-2) New Guinea C strain at multiplicity of infection of 0.5 and 1 FFU/cell, and cocultured in vitro with and without adherent PBMCs. The infectivity of DENV was assessed by immunoperoxidase staining. The viability of Huh 7it-1 cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, a tetrazole) assay and trypan blue staining. Data were statistically analyzed by Shapiro–Wilk and analysis of variance for normality significances. RESULTS The result showed that addition of PBMCs to DENV-2 infected Huh 7it-1 cells decreased the infectivity of DENV (15–37%). DENV-2 infection decreased the viability of Huh 7it-1 cells (15.5–20.8%). Despite the decrease in infectivity of DENV, the addition of PBMCs increased the Huh 7it-1 cells viability (4.5–10.2%). CONCLUSIONS Addition of PBMCs to Huh 7it-1 cells that are infected with DENV-2 decreased the infectivity of DENV and increased Huh 7it-1 cells viability.

An in vitro culture system for peripheral blood leucocytes of a sciaenid fish

1995 ◽  
Vol 5 (2) ◽  
pp. 97-107 ◽  
Author(s):  
Christina J. Lopresto ◽  
Leslie Kendall Schwarz ◽  
Karen G. Burnett
Keyword(s):  
In Vitro Culture ◽  
Peripheral Blood ◽  
Culture System ◽  
In Vitro Culture System ◽  
Peripheral Blood Leucocytes ◽  
Sciaenid Fish
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