Antibodies to cytokeratins bind to epitopes in human uterine smooth muscle cells in normal and pathological pregnancies

1995 ◽  
Vol 27 (5) ◽  
pp. 407-414 ◽  
Author(s):  
B. STIEMER ◽  
R. GRAF ◽  
H. NEUDECK ◽  
R. HILDEBRANDT ◽  
H. HOPP ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle

scholarly journals Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells

2020 ◽  
Vol 10 (03) ◽  
pp. e335-e341
Author(s):  
Arunmani Mani ◽  
John W. Hotra ◽  
Sean C. Blackwell ◽  
Laura Goetzl ◽  
Jerrie S. Refuerzo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Preterm Birth ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle ◽  
Cell Based Therapy ◽  
Increased Risk

Abstract Objective The aim of this study was to determine if mesenchymal stem cells (MSCs) would suppress the inflammatory response in human uterine cells in an in vitro lipopolysaccharide (LPS)-based preterm birth (PTB) model. Study Design Cocultures of human uterine smooth muscle cells (HUtSMCs) and MSCs were exposed to 5 μg/mL LPS for 4 hours and further challenged with 1 μg/mL LPS for a subsequent 24 hours. Key elements of the parturition cascade regulated by toll-like receptors (TLRs) through activation of mitogen-activated protein kinases (MAPKs) were quantified in culture supernatant as biomarkers of MSC modulation. Results Coculture with MSCs significantly attenuated TLR-4, p-JNK, and p- extracellular signal-regulated kinase 1/2 (ERK1/2) protein levels compared with HUtSMCs monoculture (p = 0.05). In addition, coculture was associated with significant inhibition of proinflammatory cytokines interleukin (IL)-6 and IL-8 (p = 0.0001) and increased production of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β1 (p = 0.0001). Conclusion MSCs appear to play a role in significantly attenuating LPS-mediated inflammation via alteration of down-stream MAPKs. MSCs may represent a novel, cell-based therapy in women with increased risk of inflammatory-mediated preterm birth.

Forskolin inhibition of K+ current in pregnant rat uterine smooth muscle cells

1993 ◽  
Vol 240 (2-3) ◽  
pp. 169-176 ◽  
Author(s):  
Yoshihito Inoue ◽  
Keiichi Shimamura ◽  
Nicholas Sperelakis
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Pregnant Rat ◽  
Uterine Smooth Muscle ◽  
K Current

scholarly journals Pharmacologic characterization of the oxytocin receptor in human uterine smooth muscle cells

2000 ◽  
Vol 129 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Atsuo Tahara ◽  
Junko Tsukada ◽  
Yuichi Tomura ◽  
Koh-ichi Wada ◽  
Toshiyuki Kusayama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Oxytocin Receptor ◽  
Uterine Smooth Muscle

The characterization of human uterine smooth muscle cells in culture

1979 ◽  
Vol 196 (3) ◽  
Author(s):  
Leonard Rifas ◽  
Jane Fant ◽  
MaynardH. Makman ◽  
Sam Seifter
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle ◽  
Cells In Culture

480 Implications of cellular ERalpha/ERbeta ratio in uterine smooth-muscle cells carcinogenesis

2010 ◽  
Vol 8 (5) ◽  
pp. 123
Author(s):  
Y. Rodríguez ◽  
D. Báez ◽  
F. Montes de Oca ◽  
C. García ◽  
I. Dorta ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle
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