Enhanced accumulation of toxic compound in yeast cells having high glycolytic activity: a case study on the safety of genetically engineered yeast

2007 ◽  
Vol 30 (2) ◽  
pp. 141-146 ◽  
Author(s):  
TOMOKO INOSE ◽  
KOUSAKU MURATA
Keyword(s):  
Genetically Engineered ◽  
Yeast Cells ◽  
Toxic Compound ◽  
Glycolytic Activity ◽  
Engineered Yeast

Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24
Keyword(s):  
Human Insulin ◽  
Manufacturing Process ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Enzymatic Modification ◽  
Engineered Yeast ◽  
Direct Biosynthesis

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin

2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Lipid Production ◽  
Dry Weight ◽  
Genetically Engineered ◽  
Biodiesel Production ◽  
Yeast Cells ◽  
Gene Encoding ◽  
Direct Production ◽  
Inulinase Gene ◽  
Inulinase Activity ◽  
Engineered Yeast

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.

Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes

2009 ◽  
Vol 30 (19) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kosuke Ino ◽  
Yusuke Kitagawa ◽  
Tsuyoshi Watanabe ◽  
Hitoshi Shiku ◽  
Masahiro Koide ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Interdigitated Array Electrodes ◽  
Engineered Yeast ◽  
Interdigitated Array

Genetically engineered yeast cells and their applications

1995 ◽  
Vol 82-83 ◽  
pp. 815-822 ◽  
Author(s):  
Denis Pompon ◽  
Alain Perret ◽  
Aouatef Bellamine ◽  
Romuald Laine ◽  
Jean-Charles Gautier ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Yeast Cells ◽  
Engineered Yeast

Genetically Engineered Endophytes as Biocontrol Agents: A Case Study from Industry

1991 ◽  
pp. 401-411 ◽  
Author(s):  
Jed W. Fahey ◽  
Michael B. Dimock ◽  
Steven F. Tomasino ◽  
Jean M. Taylor ◽  
Peter S. Carlson
Keyword(s):  
Genetically Engineered ◽  
Biocontrol Agents

Enhanced arsenic accumulation by engineered yeast cells expressingArabidopsis thaliana phytochelatin synthase

2007 ◽  
Vol 99 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Shailendra Singh ◽  
Wonkyu Lee ◽  
Nancy A. DaSilva ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Yeast Cells ◽  
Phytochelatin Synthase ◽  
Arsenic Accumulation ◽  
Engineered Yeast

How Malleable is the ‘Gold Standard’?: A Case Study in Respect of South Africa’s Access to Information Regime

2013 ◽  
Author(s):  
Wilhelm Peekhaus
Keyword(s):  
South African ◽  
Gold Standard ◽  
Genetically Engineered ◽  
Access To Information ◽  
Government Information ◽  
Genetically Engineered Plants ◽  
Société Civile ◽  
Environmental Organisation ◽  
Information Regime

This paper examines the difficulties encountered by Biowatch, a South African civil society environmental organisation, in its attempts to obtain access to government information in respect of genetically engineered plants. The elaboration of the case is based on interviews conducted with the Director of Biowatch and the organisation’s legal counsel.Cette communication porte sur les difficultés rencontrées par Biowatch, une organisation environnementale de la société civile sud-africaine, lors de ses tentatives d’obtenir accès aux données gouvernementales relativement aux plantes issues du génie génétique. L’élaboration du cas se base sur des entrevues menées auprès du directeur de Biowatch et le conseiller juridique de l’organisation.

Purification of δ-aminolevulinate dehydratase from genetically engineered yeast

Yeast ◽  
1990 ◽  
Vol 6 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Leda M. Borralho ◽  
Claudio H. D. Ortiz ◽  
Anita D. Panek ◽  
James R. Mattoon
Keyword(s):  
Genetically Engineered ◽  
Engineered Yeast ◽  
Aminolevulinate Dehydratase

Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells

2014 ◽  
Vol 446 ◽  
pp. 37-43 ◽  
Author(s):  
Jun Ishii ◽  
Asami Oda ◽  
Shota Togawa ◽  
Akira Fukao ◽  
Toshinobu Fujiwara ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Receptor Type ◽  
Fluorescence Sensing ◽  
Neurotensin Receptor ◽  
Engineered Yeast

Selection of antigens for use in a virus-vectored immunocontraceptive vaccine: PH-20 as a case study

1997 ◽  
Vol 9 (1) ◽  
pp. 117 ◽  
Author(s):  
Michael K. Holland ◽  
Jason Andrews ◽  
Hannah Clarke ◽  
Clayton Walton ◽  
Lyn A. Hinds
Keyword(s):  
Oryctolagus Cuniculus ◽  
Genetically Engineered ◽  
Myxoma Virus ◽  
Wild Rabbit ◽  
Pest Species ◽  
Genes Encoding ◽  
Overall Efficiency ◽  
Selection Of ◽  
Sperm Antigen

The European wild rabbit (Oryctolagus cuniculus) has become the major agricultural and environmental pest species in Australia. Current methods of rabbit control are lethal procedures which are increasingly questioned for their overall efficiency, applicability, specificity, cost and humaneness. New initiatives are required. One such initiative is virus-vectored immunocontraception. In this approach, the lagomorph-specific myxoma virus will be genetically engineered to include genes encoding components of rabbit gametes which can induce an immune response that causes infertility. Central to such a strategy is the ability to identify antigens capable of inducing an immunocontraceptive response. A strategy for identifying such antigens has been described previously. A case study of one sperm antigen, PH-20, is reported here. The issues involved in developing this antigen to the stage where it could be considered as a candidate for insertion into a recombinant myxoma virus with the ultimate goal of testing for immunocontraceptive efficacy are discussed. Techniques for inserting genes into myxoma virus have been described previously. The knowledge gained from research with this particular antigen are broadly applicable to other antigens used for both immunocontraceptive vaccines in general and, specifically, for virus-vectored immunocontraception.

Sign in / Sign up

Export Citation Format

Share Document